• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Molecular systematical Studies of conifer using DNA sequence information

Research Project

Project/Area Number 09460070
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 林学
Research InstitutionKyushu University

Principal Investigator

SHIRAISHI Susumu  Kyushu University, Faculty of Agriculture, Associate Professor, 農学部, 助教授 (70226314)

Co-Investigator(Kenkyū-buntansha) UEMURA Kazuhiko  National Science Museum, Department of Geology, Head of Laboratory, 地学研究部, 研究員 (50000138)
SUZUKI Mitsuo  Tohoku University, Division of Science, Professor, 大学院理学研究科, 教授 (80111483)
TAKATA Katsuhiko  Kyushu University, Faculty of Agriculture, Assistant Professor, 農学部, 助手 (50264099)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥11,600,000 (Direct Cost: ¥11,600,000)
Fiscal Year 1998: ¥4,900,000 (Direct Cost: ¥4,900,000)
Fiscal Year 1997: ¥6,700,000 (Direct Cost: ¥6,700,000)
Keywordsmolecular systematics / molecular Phylogeny / conifer / Coniferopsida / chloroplast DNA / DNA sequence / nuclear DNA / RAPD / 球果網植物 / 植物分類 / 系統樹 / マツ科 / マツ属 / 塩基配列情報 / 球果綱植物 / rbcL / atpA / petA
Research Abstract

The molecular evolution of conifers was elucidated by using DNA sequence information on chloroplast DNA and/or 18S rDNA gene on nuclear DNA. The main results are as follows:
1.Phylogeny of conifer at family level: The DNA sequences (ca, 3,000 bps) of three chloroplast DNA genes, atpA, petA and rbcL were decided in eight coniferous families. A new finding that was different from the traditional hypothesis was revealed from maximum likelihood analysis of sequence information.
2.Phylogeny of the family Cupressanceae: The DNA sequence of the three above-mentioned genes was decided in 11 genera. Chamaecyparis nootkatensis which had been included to the genus Chamaecyparis up to now was classified into the genus Cupressus. In addition, the 18S rDNA sequences of six species in Chamaecyparis was decided. These species can identify using a specific sequence.
3.Evolution of the family Pinaceae: The DNA sequences of six genes (atpA, petA, rbcL, rpoA, rps2 and rps3) on chloroplast DNA were decided for nine genera which composed the Pinaceae. The Phylogeny of the family was analyzed based on the sequence information. As a result, the family Pinaceae was classified into four subfamilies.
4. The construction of a new classification system in the genus Pinus: In Pinus, the largest taxon in the conifer, the classification system based on the phylogeny was restructured from chloroplast DNA information. A molecular phylogenetic tree was made from DNA information of three genes (atpA, rbcL and rpoA) on chloroplast DNA. A new classification system which was greatly different from the traditional classification systems was proposed based on this tree. Four sections and 11 subsections of the new system were composed of the species which were distributed in the neighborhood. The biogeographic factor strongly influenced the evolution of the Pinus.

Report

(3 results)
  • 1999 Final Research Report Summary
  • 1998 Annual Research Report
  • 1997 Annual Research Report
  • Research Products

    (26 results)

All Other

All Publications (26 results)

  • [Publications] 渡辺敦史: "PCR-SSCP分析を用いたアカマツとクロマツの葉緑体ゲノム型の簡便判定法"日本林学会誌. 79(3). 155-156 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Maeda,H.: "An identification of chloroplast DNA haplotypes of Larix Kaempferi and L.gmelinii var.japonica using fluorescence-based PCR-SSCP analysis of rbcL gene"Journal of Forest Research. 2(3). 187-188 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 白石進: "針葉樹類における系統進化と種分化"基礎育種学シンポジウム報告. 17. 20-28 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 白石進: "DNAがあかすアイノコマツの正体"林木の育種. 183. 1-6 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 白石進: "DNAが語るマツの来た道-進化と系統分類-"林木の育種. 185. 31-35 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 楢崎康ニ: "カラマツのRAPD分析における多型/単型バンドの出現頻度"日本林学会九州支部研究論文集. 50. 65-66 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 前田一: "RAPD分析の再現性に及ぼす鋳型DNA純度の影響"日本林学会九州支部研究論文集. 50. 67-68 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 渡辺敦史: "レバノンシーダーのrbcL遺伝子の塩基配列"日本林学会九州支部研究論文集. 50. 69-70 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 磯田圭哉: "RAPDによるモミ・ウラジロモミ識別マーカーのスクリーニング"日本林学会九州支部研究論文集. 51. 21-22 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 渡辺敦史: "RAPD分析を用いた東アジア産マツの系統関係の解明"日本林学会九州支部研究論文集. 51. 23-24 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 渡辺敦史: "マツ近縁種間における葉緑体DNAスペンサー領域のシークエンス変異-Virginia pineとSand pine間の変異-"日本林学会九州支部研究論文集. 52. 53-54 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Watanabe, A., Maeda, H., and Shiraishi, S.: "A simple indentification of chloroplast genome types between Pinus densiflora Sieb. Et Zucc. And P. thunbergii Parl. Using PCR-SSCP analysis."J. Jpn. For. Soc.. 79(3). 155-156 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Maeda, H., and Shiraishi, S.: "An identification of chloroplast DNA haplotypes of Larix Kaempferi and L.gmelinii var. japonica using fluorescence-based PCR-SSCP analysis of rbcL gene."J. For. Res.. 2(3). 187-188 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Shiraishi, S.: "Molecular evolution and speciation of conifer."Symposia on Basic Breeding Science. 17. 20-28 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Shiraishi, S.: "DNA systematical study of Pinus x densi-thunbergii."Forest Tree Breeding.. 183. 1-6 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Shiraishi, S.: "DNA phylogeny of the genus Pinus."Forest Tree Breeding.. 185. 31-35 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Narasaki, K. and Shiraishi, S.: "Frequencies that polymorphic/monomorphic PCR products appear in RAPD analysis of Larix kaempferi."Trans. 50th Mtg. Kyushu Branch Jpn. For. Soc.. 65-66 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Maeda, H., and Shiraishi, S.: "Influence of DNA quality on reproducibility of RAPD patterns."Trans. 50th Mtg. Kyushu Branch Jpn. For. Soc.. 67-68 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Watanabe, A., and Shiraishi, S.: "The rbcL gene sequence of Cedrus libani."Trans. 50th Mtg. Kyushu Branch Jpn. For. Soc.. 69-70 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Isoda, K.., and Shiraishi, S.: "Screening of RAPD markers for discrimination two Abies species, A.firma and A.homolepis."Trans. 51th Mtg. Kyushu Branch Jpn. For. Soc.. 21-22 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Watanabe, A., and Shiraishi, S.: "The phylogenetic relationships of genus Pinus distributed in east Asia using RAPD analysis."Trans. 51th Mtg. Kyushu Branch Jpn. For. Soc.. 23-24 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Watanabe, A., and Shiraishi, S.: "Sequence variation of chloroplast DNA spacer region detected between two closed related Pinus species."Trans. 52th Mtg. Kyushu Branch Jpn. For. Soc.. 53-54 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 渡辺敦史: "マツ近縁種間における葉緑体DNAスペーサー領域のシークエンス変異-pineとSand pine間の変異-" 日本林学会九州支部研究論文集. 52(印刷中). (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] 磯田圭哉・白石 進: "PARDによるモミ・ウラジロモミ識別マーカーのスクリーニング" 日本林学会九州支部研究論文集. 51(印刷中). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] 渡辺敦史・白石 進: "PARD分析を用いた東アジア産マツの系統関係の解明" 日本林学会九州支部研究論文集. 51(印刷中). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] 白石 進: "DNAが語るマツの来た道 -進化と系統分類-" 林木の育種. 185. 31-35 (1997)

    • Related Report
      1997 Annual Research Report

URL: 

Published: 1997-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi