| Project/Area Number |
09460084
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General fisheries
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| Research Institution | The University of Tokyo |
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Principal Investigator |
WAKABAYASHI Hisatsugu The University of Tokyo, Graduate School of Agricultural and Life Science, Professor, 大学院・農学生命科学研究科, 教授 (00011932)
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| Co-Investigator(Kenkyū-buntansha) |
YOKOYAMA Hiroshi The University of Tokyo, Graduate School of Agricultural and Life Science, Instr, 大学院・農学生命科学研究科, 助手 (70261956)
OGAWA Kazuo The University of Tokyo, Graduate School of Agricultural and Life Science, Assoc, 大学院・農学生命科学研究科, 助教授 (20092174)
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| Project Period (FY) |
1997 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥12,400,000 (Direct Cost: ¥12,400,000)
Fiscal Year 1998: ¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 1997: ¥8,900,000 (Direct Cost: ¥8,900,000)
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| Keywords | particle / uptake / clearance / skin / microinjury / macrophage / sporozoa / fluorescent labeling / グルゲア胞子 / 損傷 / レクチン |
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| Research Abstract |
1) Fluorescent latex beads were administered to 3-10 g rainbow trout by bath immersion, followed by time series sampling and histological examination. When the live fish exposed to the beads was dipped in 0.05% trypan blue before sampling, the patches of beads were observed at the skin area stained with trypan blue. In the skin examined histologically, there were the beads adhering to the superficial epithelial cells at the edge of wounds. During healing from wounds, the damaged cells and the beads sloughed together from the surface. However, the migrating epithelial cells which would repair the wounds displayed intensive phagocytosis of the beads at the edge of the wounds. The beads were also found in the intracellular gap in the dermis scaled with the migrating epithelium. Thus the microinjury in the skin are implicated as sites of particle uptake during bath immersion.
2) Sevcral fluorescent labeling techniques were developed to track infectious organisms on fish hosts. Standardized
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staining methods of Uvitex2B and CFSE were applied for microsporidian and actinospore, respectively. In transmission experiments with labeled spores suggested that micro-sporidian (G.plecoglossi) invaded fish passively through microinjury on the body surface, and that actinospores (T.hovorkai, M.arcticus) invaded actively through gills and/or skin.
3) G.plecoglossi spores had lectin-reactive components. Phagocytosis of the spores by ayu head kidney macrophages was significantly inhibited after ConA treatment. Percentage phagocytosis was significantly reduced when the spores were treated with ConA.Hydrogen peroxide production was suppressed by ConA treatment of spores. In the experimental infection of ayu, the number of cysts was smaller in ConA-treated spore administrated group than in untreated control group. It was suggested that ConA could block the binding of G.plecoglossi s spore with ayu macrophages and that their phagocytosis of the spores played an important role in migration of the spore in the infected fish. Less
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