| Project/Area Number |
09460087
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General fisheries
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| Research Institution | Hiroshima University |
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Principal Investigator |
UEMATSU Kazumasa Hiroshima Univ., Fac. Appl. Biol. Sci., Prof., 生物生産学部, 教授 (00116542)
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| Project Period (FY) |
1997 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥10,200,000 (Direct Cost: ¥10,200,000)
Fiscal Year 1999: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1998: ¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 1997: ¥5,900,000 (Direct Cost: ¥5,900,000)
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| Keywords | CARP / FICTIVE SWIMMING / MID BRAIN / NFLM / SPINAL CORD / MOTOR NEURON / NEURONAL LABELING / NEURONAL ACTIVITY / 運動誘起 / 遊泳 / 運動 / 脳 / fictive swimming / 仮想的遊泳活動 |
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| Research Abstract |
1. By the retrograde neuronal labeling with Biocytin applied to the spinal cord of the carp , nearly 120 neurons projecting to the spinal cord were labeled in the nucleus fasciculus longitudinalis medialis (Nflm) of the both sides, while nearly 400 neurons with glutaminergic -immunoreaclivity were observed in Nflm of the both sides. Size distribution of glutaminergic neurons included that of the labeled reticurospinal neurons, suggesting that neurons that participate in initiation of fish swimming must be glutaminergic.
2. Neural activities of Nflm neurons and the spinal motor neurons were recorded simultaneously in the carp paralyzed with curare. During fictive swimming, several kinds of Nflm neurons exhibiting different neuronal activities were observed. 1) tonically firing neurons during an episode of swimming. 2) phaslcally fired neurons firing on the phase or out of phase with motor neuron bursts. 3) neurons which cease firing during an episode of swimming. 4) neurons apparently irrelevant to the swimming.
3. Recorded Nflm neurons were labeled with Neurobiotin released electrophoretically from recording electrodes filled with the tracer at the end of each experiment. This procedure revealed that the Nflm neurons with different activity patterns were distributed uniformly in Nflm.
4. Some of these neuronal activities were also observed in free moving fish that were fitted with electrodes in Nflm and an OP amp. on the head.
5. I guess that the tonic neurons may initiate and maintain ordinary swimming with body undulation, while the phasic neurons of different types may modulate swimming to slow down, speed up, turn or stop.
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