Physiological and molecular biological study on the mechanisms for VFA-induced inhibition of GH release from somatotrophs
| Project/Area Number |
09460122
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied animal science
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| Research Institution | Tohoku University |
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Principal Investigator |
KATOH Kazuo AGRICULTURE TOHOKU UNIVERSITY,ASSOC.PROF., 農学部, 助教授 (60091831)
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| Co-Investigator(Kenkyū-buntansha) |
HAGINO Akihiko AGRICULTURE TOHOKU UNIVERSITY,RES.ASSOC., 農学部, 助手 (80156249)
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| Project Period (FY) |
1997 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥5,300,000 (Direct Cost: ¥5,300,000)
Fiscal Year 1998: ¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1997: ¥3,400,000 (Direct Cost: ¥3,400,000)
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| Keywords | VFA / GH (GROWTH HORMONE) / GH SECRETING CELLS / SOMATOTROPHS / 分泌 |
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| Research Abstract |
(1) Effects of VFA on the basal and GHRH-induced GH release
When the cells incubated with butyrate or propionate for 72 hrs were stimulated with GHRH, GH release was significantly suppressed compared with the cells cultured in a medium without a fatty acid.
(2) Effects of VFA on membrane Ca current
When membrane potential was depolarized in a step manner, inward current was increased via the cell membrane. This current, which was completely abolished by nifedipine, was suppressed in a medium containing butyrate.
(3) Effects of VFA on cellular Ca ion concentrations
Butyrate itself slightly increased, but it rater suppressed GHRH-induced increase in cellular Ca concentration.
(4) Effects of VFA on GHmRNA content
Butyrate suppressed GHmRNA content.
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Report
(3 results)
Research Products
(16 results)
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[Publications] Matsunaga, N., Arakawa, N.T., Goka, T., Nam, K.-T., Ohneda, A., Sasaki, Y.and Katoh, K.: "Effects of ruminal infusion of volatile fatty acids on plasma concentration of growth hormone and insulin in sheep" Domestic Animal Endocrinology. (in press).
Description
「研究成果報告書概要(欧文)」より
Related Report
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