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MECHANISMS OF HOSTSPECIFICITY OF CANINE DISTEMPER VIRUS

Research Project

Project/Area Number 09460136
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Basic veterinary science/Basic zootechnical science
Research InstitutionUNIVERSITY OF TOKYO

Principal Investigator

KAI Chieko  INSTITUTE OF MEDICAL SCIENCE, UNIVERSITYOF TOKYO, PROFESSOR, 医科学研究所, 教授 (10167330)

Co-Investigator(Kenkyū-buntansha) MIYAZAWA Takayuki  GRADUATE SCHOOL OF AGRICULTURAL AND LIFE SCIENCES, UNIVERSITY OF TOKYO, ASSISTANT PROFESSOR, 大学院・農学生命科学研究科, 助手 (80282705)
Project Period (FY) 1997 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥13,200,000 (Direct Cost: ¥13,200,000)
Fiscal Year 1999: ¥4,300,000 (Direct Cost: ¥4,300,000)
Fiscal Year 1998: ¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 1997: ¥5,200,000 (Direct Cost: ¥5,200,000)
Keywordsdistemper / virus / canine / wild animals / Haemagglutinin / CDV / RFLP / reverse genetics / リバースジェネティックス / アザラシ / タヌキ / ルシフェラーゼ / H protein / F protein / 野外株 / モノクローナル抗体
Research Abstract

Recently, the incidence of canine distemper (CDV) in domestic dogs has increased in the world. We isolated numbers of viruses from affected dogs. Genetic analysis of the Haemagglutinin (H) proteins revealed that the recent isolated strains have an increased number of potential sites for glycosylation. We also revealed that the prevalence of the disease in Japan mainly caused by new types of CDV which could be distinguished from vaccines and old types of CDV by restriction fragment length polymorphism of H genes. By the analyses of cross-reactivity to monoclonal antibodies, at least one neutralizing epitope of the H protein of prevalent viruses was suggested to be changed. The full genome of the Yanaka strain, a new isolate was sequenced. It contains 15,690bp of nucleotides and deduced amino acid sequence had approximately 92% of homology compared with that of vaccine strain. The reverse genetics of Mononegavirales is an innovative technique for the rescue of infectious viruses. We conducted to develop this system on CDV and succeeded to recover recombinant CDV from the cDNA of CDV with unique restriction enzyme sites between structural genes for further basic and applied studies. On the other hand, we conducted the epidemiological survey of morbilliviruses on aquatic animals in the coast of Japan. Serological analysis of more than 300 animals including cetaceans and pinnipeds indicated that the pinnipeds appeared to have been infected with PDV-like virus before 1988 and cetaceans with DMV-like 1978 along the coast of Japan. The genetic and serological analyses of a newly isolated CDV from a raccoon dog in Japan revealed that only four differences in amino acids sequence of H protein indicating that the virus spreading in Japanese raccoon dogs belongs to the same group as the CDV strains causing recent outbreaks in dogs.

Report

(4 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • 1997 Annual Research Report
  • Research Products

    (35 results)

All Other

All Publications (35 results)

  • [Publications] Iwatsuki,K., et al.: "Molecular and phylogenetic analyses of the haemagglutinin proteins of field isolates from dogs naturally infected with canine distempre virus."J. Gen. Virol.. 78. 373-380 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Okita, M., et al.: "Histopathological features of canine distemper recently observed in Japan."J. Comp. Pathol.. 116. 403-408 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yoshida, E., et al.: "Molecular analysis of the nucleocapsid protein of recent isolates of canine distemper virus in Japan."Vet. Microbiol.. 59. 237-244 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Nakamura, K., et al.: "Molecular cloning and sequence analysis of the phosphoprotein (P) gene of the lapinized rinderpest virus."Virus Gene. 18. 175-244 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yoshida, E., et al.: "Epitopes and nuclear localization analysis of canine distemper virus nucleocapsid protein by expression of its deletion mutants."Vet. Microbiol.. 66. 313-320 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Iwatsuki,K., et al.: "Antigenic differences in the H proteins of canine distemper viruses."Vet. Microbiol.. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 甲斐知恵子: "モービリウイルス-人間社会・動物社会双方向エマージングウイルス-"岩波書店 科学。特集「現代社会を揺るがす感染症」. 6 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 甲斐知恵子: "ウマからヒトに感染するモービリウイルス感染症"裳華房 遺伝. 5 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Iwatsuki, K., et. al.: "Molecular and phylogenetic analyses of the haemagglutinin proteins of field isolates from dogs naturally infected with canine distemper virus."J. Gen. Virol.. 78. 373-380 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Okita, M., et. al.: "Histopathological features of canine distemper recently observed in Japan."J. Comp. Pathol.. 116. 403-408 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yoshida, E., et. al.: "Molecular analysis of the nucleocapsid protein of recent isolates of canine distemper virus in Japan."Vet. Microbiol.. 59. 237-244 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Nakamura, K., et. al.: "Molecular cloning and sequence analysis of the phosphoprotein (P) gene of the lapinized rinderpest virus."Virus Gene. 18. 175-178 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yoshida, E., et.al.: "Epitopes and nuclear localization analysis of canine distemper virus nucleocapsid protein by expression of its deletion mutants."Vet. Microbiol.. 66. 313-320 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Iwatsuki, K., et. al.: "Antigenic differences in the H proteins of canine distemper viruses."Vet. Microbiol.. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Nakamura, K. et al.: "Molecular cloning and sequence analysis of the phosphoprotein (P)gene of the lapinized rinderpest virus"Virus Gene. 18(2). 175-178 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Yoshida, E. et al.: "Epitopes and nuclear localization analysis of canine distemper virus nucleocapsid protein by expression of its deletion mutants"Vet. Microbiol. 66. 313-320 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Wakasa, C. et al.: "Sequence analysisi of the genes encoding the phophoprotein of recent isolates of canine distemper virus in Japan"J. Vet. Med. Sci.. 62(1). 97-101 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Iwatsuki, K. et al.: "Establishment of a persistent mutant of canine distemper virus"Microbe. Infect.. 1(12). 987-991 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Ohashi, K. et al.: "A canine distemper virus epidemic in raccoon dogs (Nyctereutes procyonoides viverrinus) in Japan"Vet. Record.. (in press).

    • Related Report
      1999 Annual Research Report
  • [Publications] Iwatsuki, K. et al.: "Antigenic differences in the H proteins of canine distemper viruses"Vet. Microbiol.. (in press).

    • Related Report
      1999 Annual Research Report
  • [Publications] 甲斐知恵子: "ウマからヒトに感染するモービリウイルス感染症"遺伝 裳華房. 5 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 甲斐知恵子: "野生動物でのエマージングウイルス"SUT 東京理科大学出版会. 5 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Yoshida,E.,et al.: "Epitopes and nuclear localization analysis of canine distemper virus nucleocapsid protein by expression of its deletion mutants." Vet.Microbiol.(In press).

    • Related Report
      1998 Annual Research Report
  • [Publications] Nakamura,K.,et al.: "Molecular cloning and sequence analysis of the phosphoprotein (P) gene of the lapinized rinderpest virus." Virus Gene. (in press).

    • Related Report
      1998 Annual Research Report
  • [Publications] Yamanouchi,K.,et al.: "New approaches to the development of virus vaccines for veterinary use." Rev.Sci.Tech.Off.Int.Epiz.17(3). 641-653 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Ohishi,K.,et al.: "Immunogenicity of recombinant vaccinia virus(rVV)expressing rinderpest virus nucleocapsid protein in mice." J.Vet.Med.Sci.60(5). 655-656 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Yoshida,E.,et al.: "Molecular analysis of the nucleocapsid protein of recent isolates of canine:distemper virus in Japan." Vet.Microbiiol.59. 237-244 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Nakamura,K.,et al.: "Immunization of rabbits against systemic rinderpest virus infection by vaccinia virus expressing the nucleoprotein of rinderpest virus." Comp.Immunol.Microbiol.Infect.Dis.21. 91-99 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 甲斐知恵子: "ウマからヒトに感染するモービリウイルス感染症" 遺伝, 5 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Iwatsuki,K., et al.: "Molecular and phylogenetic analyses of the hemagglutinin proteins of field isolates of canine distemper virus from naturally infected dogs." Journal of General Virology. 78. 373-380 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Okita,M., et al.: "Histopathological features of canine distemper recently observed in Japan." Journal of Comparative Pathology. 116. 403-408 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Shin,Y.-S., et al.: "Expression of the nucleocapsid protein gene of the canine distemper virus." Journal of Veterinary Medical Science. 59. 51-53 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 甲斐知恵子: "特集:現代社会を揺るがす感染症<ウイルス編>モ-ビリウイルス,-人間社会・動物社会双方向エマージングウイルス-" 科学(岩波書店). 67. 143-148 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Iwatsuki,K., et al.: "The nucleotide and predicted amino acid sequence of the fusion protein of recent isolates of canine distemper virus in Japan." Journal of Veterinary Medical Science. 60. 381-385 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yoshida,E., et al.: "Molecular analysis of the nucleocapsid protein of recent isolates of canine distemper virus in Japan." Veterinary Microbiology. (In press).

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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