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A study on the prevention and treanment of feline immunodeficiency virus infection

Research Project

Project/Area Number 09460144
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied veterinary science
Research InstitutionThe University of Tokyo

Principal Investigator

TSUJIMOTO Hajime  The University of Tokyo, Graduate School of Agricultural and Life Sciences, Professor, 大学院・農学生命科学研究科, 教授 (60163804)

Co-Investigator(Kenkyū-buntansha) HASEGAWA Atsuhiko  Nihon University, Faculty of Bioresource Sciences, Professor, 生物資源科学部, 教授 (90011923)
MIYAZAWA Takayuki  The University of Tokyo, Graduate School of Agricultural and Life Sciences, Assi, 大学院・農学生命科学研究科, 助手 (80282705)
WATARI Toshihiro  The University of Tokyo, Graduate School of Agricultural and Life Sciences, Assi, 大学院・農学生命科学研究科, 助手 (50220950)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥13,400,000 (Direct Cost: ¥13,400,000)
Fiscal Year 1998: ¥4,600,000 (Direct Cost: ¥4,600,000)
Fiscal Year 1997: ¥8,800,000 (Direct Cost: ¥8,800,000)
KeywordsFeline immunodeficiency virus / Subtype / Chemokine / SDF-1B / Apoptosis / IL-12 / Viral load / QC-PCR / ネコ免疫不全ウイルス / ワクチン / ウイルス増殖抑制剤 / サイトカイン / エンベロープ蛋白 / 抗酸化剤
Research Abstract

Nucleotide sequence analysis of feline imunodeficiency virus (FIV) env gene obtained from multiple districts in Japan showed that subtypes B and D viruses were predominant in the eastern and western parts of Japan, respectively, and subtypes A and C viruses were occasionally found in some specific areas. Molecular clones of feline CC-and CXC-chemokines, which have been identified as ligands of co-receptors for HIV, were isolated and characterized. Of these chemokines, SDF-1beta was shown to inhibit the growth of FIV in cat T-lymphoid cells. Moreover, T22, a newly synthesized compound which binds to CXC-chemokine receptor, also inhibited FIV growth.
On the other hand, apoptosis induced by FIV infection is known to play an important role for the pathogenesis of immunodeficiency. Antioxidants, N-acetylcysteine and ascorbic acid, were shown to inhibit apoptosis induced by FlV infection in vitro. Furthermore, IL-12 and IL-10 were added to the in vitro apoptosis-induction system by Fly. IL-12 was shown to inhibit FIV growth and apoptosis, however, IL-lO did not have such effect. The inhibitory effect of IL-12 for virus growth and apoptosis was found to be mediated by the expression of IFN-gamma.
Next, to examine the effect of these compounds for inhibition of FIV growth and apoptosis, plasma viral RNA load in FLY-infected cats was quantified with QC-PCR.As a result, the plasma viral RNA load was less than 105 copies/ml in cats in asymptomatic carrier stage but higher than 107 copies/ml in cats in AIDS stage. By using the quantification system for plasma FIV RNA, we are continueing the investigation on the kinetics of the viral RNA load and effect of anti-viral drugs in FIV-infected cats.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] Nishimura,Y.et al.: "Genetic heterogeneity in the env gene of FIV clones obtained from various districts in Japan." Virus Res.57. 101-112 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Mortola,E.et al.: "Effect of interleukin-12 and interleukin-10 on the virus replication and apoptosis in T-cells infected with feline immunodeficiency virus." J.Vet.Med.Sci.60. 1181-1185 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Mortola,E.et al.: "Inhibition of apoptosis and virus replication in feline immunodeficiency virus-infected cells by N-acetylcysteine and ascorbic acid." J.Vet.Med.Sci.60. 1187-1193 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Mizuno,T.et al.: "Molecular cloning of feline Fas antigen and Fas ligand cDNAs." Vet.Immunol.Immunopathol.65. 161-172 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Cho, K.W.et al.: "Cloning and mapping of cat(Felis catus)immunoglobulin and T-cell receptor genes." Immunogenetics. 47. 226-233 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Endo,Y.et al.: "Clinocopathological and immunological characteristics of 6 cases with granular lymphocyte tumors in cats." Comp.Immunol.Microbiol.Infec.Dis.21. 27-42 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nishimura, Y.et al.: "Genetic heterogeneity in the env gene of FIV clones obtained from various districts in Japan." Virus Res.57. 101-112 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Mortola, E.et al.: "Effect of interleukin-12 and interleukin-10 on the virus replication and apoptosis in T-cells infected with feline immunodeficiency virus." J.Vet.Med.Sci. 60. 1181-1185 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Mortola, E.et al.: "Inhibition of apoptosis and virus replication in feline immunodeficiency virus-infected Cells by N-acetylcysteine and ascorbic acid." J.Vet.Med.Sci.60. 1187-1193 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Mizuno, T.et al.: "Molecular cloning of feline Fas antigen and Fas ligand cDNAs." Vet.Immunol.Immunopathol. 65. 161-172 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Cho, K.W.et al.: "Cloning and mapping of cat (Felis cats) immunoglobulin and T-Cell receptor genes." Immunogenetics. 47. 226-233 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Endo, Y.et al.: "Clinocopathological and immunological characteristics of 6 cases with granular lymphocyte tumors in cats." Comp.Immunol.Microbiol Infec.Dis.21. 27-42 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nishimura,Y.et al: "Genetic heterogeneity in the env gene of FIV clones obtained from various districts in Japan." Virus Res.57. 101-112 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Mortola,E.et al: "Effect of interleukin-12 and interleukin-10 on the virus replication and apoptosis in T-cells infected with feline immunodeficiency virus." J.Vet.Med.Sci.60. 1181-1185 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Mortola,E.et al: "Inhibition of apoptosis and virus replication in feline immunodeficiency virus-infected cells by N-acetylcysteine and ascorbic acid." J.Vet.Med.Sci.60. 1187-1193 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Mizuno,T.et al: "Molecular cloning of feline Fas antigen and Fas ligand cDNAs." Vet.Immunol.Immunopathol.65. 161-172 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Cho,K.W.et al: "Cloning and mapping of cat(Felis catus)immunoglobulin and T-cell receptor genes." Immunogenetics. 47. 226-233 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Endo,Y.et al: "Clinocopathological and immunological characteristics of 6 cases with granular lymphocyte tumors in cats." Comp.Immunol.Microbiol.Infec.Dis.21. 27-42 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Endo,Y., et al.: "Molecular characteristics of malignant lymphomas in cats naturally infected with feline immunodeficiency virus." Veterinary Immunology and Immunopathology. 57. 153-167 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Mizuno,T., et al.: "Apoptosis euhanced by soluble factor produced in faline immunodeficiency virus infection." Journal of Veterinary Medical Science. 59. 1049-1051 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Endo,Y., et al.: "Molecular cloning of feline CC-chemokine cDNAs." Veterinary Immunology and Immunopathology. (印刷中). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Cho,K.W., et al.: "Assignment of feline immunoglobulin heavy chain gene to chronosome B3q26 and T'cell receptor gamma chain gene to A2q12-q13." Cytogenetics and Cell Genetics. (印刷中). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Cho,K.W., et al.: "Cloning and mapping of cat (Felis catus)immunoglobulin and T-cell receptor genes." Immunogenetics. (印刷中). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Mizuno,T., et al.: "Molecular cloning of feline Fas autigen and Fas ligand and their expression in feline immunodeficiency virus infection." Veterinary Immunology and Immunopathology. (印刷中). (1998)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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