• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Studies on new diagnosis and classification of diabetes in dogs and cats

Research Project

Project/Area Number 09460148
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied veterinary science
Research InstitutionNippon Veterinary and Animal Science University

Principal Investigator

ARAI Toshiro  Nippon Vet Anim Sci Univ, Dept Vet Sci, Assoc Prof, 獣医畜産学部, 助教授 (70184257)

Co-Investigator(Kenkyū-buntansha) SAKO Toshinori  Nippon Vet Anim Sci Univ, Dept Vet Sci, Assoc Prof, 獣医畜産学部, 助教授 (70153971)
TSUCHIDA Shuichi  Nippon Vet Anim Sci Univ, Dept Vet Sci, Lecturer, 獣医畜産学部, 講師 (20217326)
SAITO Tohru r.  Nippon Vet Anim Sci Univ, Dept Vet Sci, Assoc Prof, 獣医畜産学部, 助教授 (70211258)
OHGAWARA Hisako  Tokyo Womens Med Coll, Med Res Inst, Assoc Prof, 医学部, 助教授 (10075468)
OHISHI Iwao  Nippon Vet Anim Sci Univ, Dept Vet Sci, Professor, 獣医畜産学部, 教授 (50081592)
Project Period (FY) 1997 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥5,100,000 (Direct Cost: ¥5,100,000)
Fiscal Year 1999: ¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1998: ¥2,600,000 (Direct Cost: ¥2,600,000)
Keywordsdogs and cats / diabetes / insulin / glucokinase / PCR / streptozotocin / artificial pancreas / gene diagnosis / RT-PCR法
Research Abstract

1. New diagnosis for diabetes in dogs : Plasma glucose concentrations have been used as diagnostic indicator to assess metablic conditions in dogs. White blood cell (WBC) dlucokinase (GLK) activities and mRNA were measured in streptozotocin-induced type 1 diabetic dogs. Plasma glucose concentrations decreased remarkably but mRNA qunatitation by RT-PCR was not recovered after insulin injections in the diabetic dogs. WBC GLK activities and mRNA exprerssion were considered to be a good diagnostic indicator to assess the diabetic condition in dogs.
2. Transplantation of artificial pancreas : A diffusion chamber as bioartificial endocrine pancreas (Bio-AEP) constructed by placing pancreatic endocrine cells, which were shielded by silicone, was implanted to streptozotocin (STZ)-induced diabetic rats. In the STZ diabetic rats with Bio-AEP, a return to normoglycemia was observed up to 30 weeks after implantation, without the use of any immunosuppressant. Xenoimplantation using the Bio-AEP might improve endocrine insulin release and retard the progress of diabetes.
3. Development of kits to measure plasma insulin in dogs and cats : New ELISA kits with specific antibodies against dog and cat insulin were developed. These ELISA system showed high reproducibility for measurement of plasma insulin concentrations in dogs and cats.
4. Herbal treatment for diabetes in dogs : A kind of Chinese herbal medicine, Echvaria glauca, was used for treatment of STZ-induced diabetic dogs. The oral treatment with herb gave a remarkable improvement of the diabetic condition by causing recovery of hepatic function.

Report

(4 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • 1997 Annual Research Report
  • Research Products

    (26 results)

All Other

All Publications (26 results)

  • [Publications] Hirotani, S.: "Bioartificial endocrine pancreas(Bio-AEP) for treatment of diabetes Effect of implantation of Bio-AEP on the pancreas"Cell Transplantation. 8. 399-404 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Arai, T.: "A comparison of the plasma fructose concnetrations in dogs and cats and changes in the fructose concnetrations in dogs---"Veterinary Research Communications. 23. 203-209 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Saito, T.R.: "p-Chloraamphetamine(PCA) suppresses ingestive behavior in male rats"Experimental Animals. 48. 263-267 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Washizu, T.: "Comparison of the activities of enzymes related to glycolysis and gluconeogenesis in the liver of dogs and cats"Research in Veterinary Science. 67. 205-206 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 新井敏郎: "1型糖尿病犬における血球内酵素活性の変動"日本獣医畜産大学研究報告. 48. 11-14 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Arai, T.: "Change in activities of blood cell enzymes in diabetic dogs treated with herb (Nikasyou)"Bull Nippon Vet Anim Sci Univ. 48. 15-21 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Hirotani, S., Eda, R., Kawabata, T., Fuchinoue, S., Teraoka, S., Agisi, T. and Ohgawara, H.: "Bioartificial endocrine pancreas (Bio-AEP) for treatment of diabetes : Effect of implantation of Bio-AEP on the pancress"Cell Transplantation. 8. 399-404 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Arai, Y., Hashimoto, K., Mizutani, H., Kawabata, T., Sako, T. and Washizu, T.: "A comparison of the plasma fructose concentrations in dogs and cats and changes in the fructose concentrations in dogs following intyravenous administration of fructose"Veterinary Research Communications. 23. 203-209 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Saito, R.T., Saito, M., Arai, T., Aoki-Komori, S., Taniguchi, K. and Takahashi, W.K.: "p-Chloroamphetamine (PCA) suppresses ingestive behavior in male rats."Experimental Animals. 48. 263-267 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Washizu, T., Tanaka, A., Sako, T., Washizu, M. and Arai, T.: "Com,parison of the activities of exzymes related to glycolysis and gluconeogenesis in the liver of dogs and cats."Research in Veterinary Science. 67. 205-206 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Arai, T., Takahashi, S., Tanaka, A., Kawabata, T. and Sako, T.: "Changes in blood cells enzymes activities in dogs with type 1 diabetes mellitus."Bulletin of Nippon Veterinary and Animal Science University. 48. 11-14 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Arai, T., Takahashi, S., Tanaka, A., Sasaki, N., Kawabata, T. and Sako, T.: "Changes in activities of blood cell enzymes in diabetic dogs treated with herb (Nikasyou)"Bulletin of Nippon Veterinary and Animal Science University. 48. 15-20 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Ohgawara,H.: "Bioarificial endocrine pandreas(Bio-AEP) for treatment of diabetes Effect of implantion of Bio-AEP on the pancreas"Cell Transplantation. 8. 399-404 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Arai,T.: "A comparison of the plasma fructose concnetrations in dogs and cats and ahanqes in the fructose concnetrations in dogs"Veterinary Research Communications. 23. 203-209 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Saito,T.R.: "p-Chloraamhetamine(PCA) supresses ingestive behavior in male rats"Experimental Animals. 48,4. 263-267 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Washizu,T.: "Comparison of the activities of enzymes related to giycolysis and gluconeogenesis in the liver of dogs and cats"Research in Veterinary Science. 67. 205-206 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 新井敏郎: "1型糖尿病犬における血球内酵素活性の変動"日本獣医畜産大学研究報告. 48. 11-14 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Arai,T.: "Changes in actinities of blood cell enzymes in diabetic dogs treatde with herb(Nikasyou)"Bull Nippon Vet Anim Sci Univ. 48. 15-21 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Ohgawara,H.: "Membrane immunoisolation of a diffusion for bioartificial pancreas" Artificial Organs. 22,9. 788-794 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 新井敏郎: "犬乳腺腫瘍における血清および組織アルカリホスファターゼ活性の変動" 日本獣医畜産大学研究報告. 47. 1-3 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Arai,T.: "Comparison of glucokinase activities in the peripheral leukocytes between dogs and cats" Comparative Biochemistry and Physiology. 120C,1. 53-56 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Washizu,T.: "A comparison of the activities of certain enzymes related to energy metabolism in leukocytes in dogs and cats" Veterinary Research Communications. 22,2. 187-192 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 大河原久子: "ラ氏島を用いた膵置換:Bio-artificial endocrine pancreas" 生体材料. 15,6. 330-336 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Tsuchida,S.: "AvaI polymorphism in the human transferrin gene" Human Heredity. 47. 338-341 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Arai,T.: "Glycolytic enzyme activities in leukocytes of thoroughbreds undergoing training exercise" Journal of Equine Science. 8,4. 93-96 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Washizu,T.: "A comparison of the activities of certain enzymes related to energy metabolism in leukocytes in dogs and cats" Veterinary Research Communications. 22(in print). (1998)

    • Related Report
      1997 Annual Research Report

URL: 

Published: 1998-04-01   Modified: 2022-06-06  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi