Gene expression and control mechanisms of the plant mycoplasmas

• Project/Area Number: 09460155
• Research Category: Grant-in-Aid for Scientific Research (B).
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied molecular and cellular biology
• Research Institution: The University of Tokyo
• Principal Investigator: NAMBA Shigetou Graduate School of Frontier Sciences, The University of Tokyo Professor, 大学院・新領域創成科学研究科, 教授 (50189221)
• Project Period (FY): 1997 – 2000
• Project Status: Completed (Fiscal Year 2000)
• Budget Amount: ¥13,800,000 (Direct Cost: ¥13,800,000); Fiscal Year 2000: ¥3,100,000 (Direct Cost: ¥3,100,000); Fiscal Year 1999: ¥2,500,000 (Direct Cost: ¥2,500,000); Fiscal Year 1998: ¥3,400,000 (Direct Cost: ¥3,400,000); Fiscal Year 1997: ¥4,800,000 (Direct Cost: ¥4,800,000)
• Keywords: plant mycoplasma / pathovar / Phytoplasma asteri / pathogen / microorganisms / insect vectors / bacteria / plant / 遺伝子制御ネットワーク / 野性株 / シャトルベクター / 変異株 / 分子分類 / マイコプラズマ / パルスフィールド電気泳動 / ローリングサークル型プラスミド / 一本鎖DNA結合タンパク質 / 16S rRNA遺伝子 / PCR増幅 / 分子系統学的解析 / 染色体DNA

Research Abstract

Plant mycoplasmas (phytoplasmas) are plant pathogenic bacteria inhabiting in the plant cell cytoplasm and transmitted by insect vectors. Among all known microorganisms, they are the smallest in cell size and in genome size. This study was performed to elucidate molecular mechanisms of host determination of plant mycoplasmas and to established a system for gene introduction into the bacteria. The results are expected to contribute to the control of this pathogen which causes serious damage to more than 600 plant species.

Research Products

• [Publications] Nishigawa,H.,Miyata,S.,Oshima,K.,Sawayanagi,T.,Komoto,A.,Kuboyama,T.,Matsuda,I.,Tsuchizaki,T.and Namba,S: "In planta expression of a protein encoded by the extrachromosomal DNA of phytoplasma and related to geminivirus replication proteins."Microbiology. 147. 507-513 (2001)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Lu,X.,Hirata,H.,Ugaki,M.S.and Namba,S.: "Random mutagenesis in a plant viral genome using a DNA repair-deficient mutator Escherichia coli strain."Journal of Virological Methods. (in press). (2001)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Yamaji,Y.,Lu,X.,Kagiwada,S.,Oshima,K.and Namba,S.: "Molecular evidence that a lily-infecting strain of TBV from Japan is a new potyvirus."European Journal of Plant Pathology. (accepted). (2001)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Oshima,K.,Kakizawa,S.,Nishigawa,H.,Kuboyama,T.,Miyata,S.,Ugaki,M.and Namba,S.: "A Plasmid of phytoplasma encodes a unique replication protein having both plasmid-and virus-like domains : Clue to viral ancestry of result of virus/plasmid recombination?"Virology. (in press). (2001)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Namba, S., Sawayanagi, T., Yamamoto, S., Lu, X., Kagami, T., Shiomi, T.and Tsuchizaki, T.: "Phylogenetic analysis of plant mycoplasmas occurring in the world."Japanese Journal of Mycoplasmology. 24. 100-102 (1997)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Sawayanagi, T., Kagami, T., Lu, X., Shiomi, T., Tsuchizaki, T.and Namba, S.: "Phylogenetic analysis of phytoplasmas based on 16S/23S rRNA internal transcribed spacer regions."Japanese Journal of Mycoplasmology. 24. 103-105 (1997)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Kagami, K., Shiomi, T., Tanaka, M., Fukuzawa, H., Sawayanagi, T., Sun, L., Lu, X., Tsuchizaki, T.and Namba, S.: "Pathogenicity of plant mycoplasma and extrachromosomal DNA."Japanese Journal of Mycoplasmology. 24. 106-108 (1997)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Yamamoto, Y., Sawayanagi, T., Kagami, T., Tominaga, A., Sun, L., Lu, X., Tanaka, M., Shiomi, T., Tsuchizaki, T.and Namba, S.: "Genome of plant mycoplasma."Japanese Journal of Mycoplasmology. 24. 109-110 (1997)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Shiomi, T., Tanaka, M., Sawayanagi, T., Yamamoto, S., Tsuchizaki, T.and Namba, S.: "A symptomatic mutant of onion yellows phytoplasma derived from a greenhouse-.maintained isolate"Ann.Phytopath.Soc.Japan. 64. 501-505 (1998)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Sawayanagi, T., Nishikawa, H., Horikoshi, N., Kanehira, T., Shinohara, M.and Namba, S.: "Phylogenetic analysis of Japanese Hydrangea phyllody phytoplasma using the 16S rRNA gene."Japanese Journal of Mycoplasmology. 25. 127-129 (1998)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Kuboyama, T., Lu, X., Sawayanagi, T., Kanazawa, T., Nishigawa, H., Arai, S., Oshima, K., Matsuda, I., Tsuchizaki, T.and Namba, S.: "A rolling-circle replication plasmid, pOYW1, isolated from phytoplathogenic onion yellows phytoplasma."Japanese Journal of Mycoplasmology. 25. 124-126 (1998)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Kuboyama, T., Huang, C.-C., Lu, X., Sawayanagi, T., Kanazawa, T., Kagami, T., Matsuda, I., Tsuchizaki, T.and Namba, S.: "A plasmid isolated from phytopathogenic Onion Yellows phytoplasma and its heterogeneity in thesymptomatic phytoplasma mutant."Molecular Plant Microbe Interaction. 11. 1031-1037 (1998)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Nishimura, Norio, Nakajima, S., Sawayanagi, T.and Namba, S.: "Transmission of Cryptotaenia japonica witches' broom phytoplasma and oinion yellows phytoplasma byHishimonus sellatus Uhler."Ann.Phytopath.Soc.Japan. 64. 474-447 (1998)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Kanazawa, T., Sawayanagi, T., Tanaka, M., Nishigawa, H., Kuboyama, T., Arai, S., Oshima, K., Matsuda, I., Tsuchizaki, T.: "Characterization of an isolated symptomatic onion yellows phytoplasma mutant."Japanese Journal of Mycoplasmology. 25. 121-123 (1998)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Sawayanagi, Ttoshimi, Horikoshi, N., Kanehira, T., Shinohara, M., Lu, X., Kagami, T., Huang, C.-C.and Namba, S.: ""Candidatus phytoplasma japonicum, " a new phytoplasma taxon associated with Hydrangea phyllody."International Journal of Systematic Bacteriology. 49. 1275-1285 (1999)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Uehara, Tamaki, Tanaka, M., Shiomi, T., Namba, S., Tsuchizaki, T.and Matsuda, I.: "Histopathological studies on two symptom types of phytoplasma associated with lettuce yellows."Ann.Phytopath.Soc.Japan. 65. 465-469 (1999)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Sawayanagi, T., Horikoshi, N., Kanehira, T., Shinohara, M., Oshima, K.and Namba, S.: "'Candidatus Phytoplasma japonicum', a new phytoplasma taxon associated with Japanese Hydrangea phyllody."Japanese Journal of Mycoplasmology. 26. 30-33 (1999)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Oshima, K., Kagami, T., Kuboyama, T., Kanazawa, T., Nishigawa, H., Sawayanagi, T., Tanaka, M., Matsuda, I., Tsuchizaki, T.and Namba, S.: "Characterization of an extrachromosomal DNA (OYEW1) from onion yellows phytoplasma."Japanese Journal of Mycoplasmology. 26. 34-36 (1999)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Komoto, A., Oshima, K., Kanazawa, T., Sawayanagi, T., Arai, S., Matsuda, I., Tsuchizai, T., Kuboyama, T.and Namba, S.: "Characterization of thymidylate kinase gene from OY-Phytoplasma."Japanese Journal of Mycoplasmology. 26. 37-39 (1999)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Nishigawa, H., Miyata, S., Oshima, K., Sawayanagi, T., Komoto, A., Kuboyama, T., Matsuda, I., Tsuchizaki, T.and Namba, S.: "In planta expression of a protein encoded by the extrachromosomal DNA of a phytoplasma and related to geminivirus replication proteins."Microbiology. 147. 507-513 (2001)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Oshima, K., Kakizawa, S., Nishigawa, H., Kuboyama, T., Miyata, S., Ugaki, M.and Namba, S.: "A Plasmid of phytoplasma encodes a unique replication protein having both plasmidand virus-like domains : Clue to viral ancestry or result of virus/plasmid recombination ?"Virology. (in press). (2001)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Nishigawa,H.,Miyata,S.,Oshima,K.,Sawayanagi,T.,Komoto,A.,Kuboyama,T.,Matsuda,I.,Tsuchizaki,T.and Namba,S: "In planta expression of a protein encoded by the extrachromosomal DNA of phytoplasma and related to geminivirus replication proteins."Microbiology. 147. 507-513 (2001)
• [Publications] Lu,X.,Hirata,H.,Ugaki,M.S.and Namba,S.: "Random mutagenesis in a plant viral genome using a DNA repair-deficient mutator Escherichia coli strain."Journal of Virological Methods. (in press). (2001)
• [Publications] Yamaji,Y.,Lu,X.,Kagiwada,S.,Oshima,K.and Namba,S.: "Molecular evidence that a lily-infecting strain of TBV from Japan is a new potyvirus."European Journal of Plant Pathology. (accepted). (2001)
• [Publications] Kuboyama, Tsutomu: "A plasmid isolated from phytopathogenic Onion Yellows phytoplasma and its heterogeneity in thesymptomatic phytoplasma mutant"Molecular Plant Microbe Interaction. 11. 1031-1037 (1998)
• [Publications] Lu, Xiaoyun: "The 3'-terminal sequence of RNA1 of wheat spindle streak mosaic virus Canadian isolate(WSSMV-C)"European Journal of Plant Pathology. 104. 765-768 (1998)
• [Publications] Lu, Xiaoyun: "The genome oranization of the broad bean necrosis virus(BBNV)"Archives of Virology. 143. 1335-1348 (1998)
• [Publications] Nishimura, Norio: "Transmission of cryptotaenia japonica witches' broom phytoplasma and oinion yellows phytoplasma by Hishimonus sellatus Uhler"Ann. Phytopath. Soc. Japan. 64. 474-477 (1998)
• [Publications] Sawayanagi, Toshimi: ""Candidatus phytoplasma japonicum," a new phytoplasma taxon associated with Hydrangea phyllody"Intenational Journal OF Systematic Bacteriology. 49. 1275-1285 (1999)
• [Publications] Uehara, Tamaki: "Histopathological studies on two symptom types of phytoplasma associated with lettuce yellows"Ann. Phytopath. Soc. Japan. 65. 465-469 (1999)
• [Publications] Kuboyama,T.: "A plasmid isolated from phytopathogenic Onion Yellow phytoplasma and its heterogeneity in the symptomatic phytoplasma mutant." Molecular Plant Microbe Interaction. 11. 1031-1037 (1998)
• [Publications] 塩見敏樹: "継代保存中に生じたタマネギ萎黄病ファイトプラズマの病微変異株" 日本植物病理学会報. 64. 501-505 (1998)
• [Publications] Nishimura,N.: "Transmission of Cryptotaenia japonica witches'broom phytoplasma and oinion yellows phytoplasma by Hishimonus sellatus Uhler." Annals of the Phytopathological Society of Japan. 64. 474-477 (1998)
• [Publications] 金沢登紀子: "OY-ファイトプラズマ病微変異株の分子生物学的解析" 日本マイコプラズマ学会雑誌. 25. 121-123 (1998)
• [Publications] 久保山 勉: "OY-ファイトプラズマより分離されたRCR型プフスミドpOYW1" 日本マイコプラズマ学会雑誌. 25. 123-126 (1998)
• [Publications] 澤柳利実: "16S rRNA遺伝子によるアジサイ葉化病ファイトプラズマの系統解析" 日本マイコプラズマ学会雑誌. 25. 127-129 (1998)
• [Publications] 難波成任: "世界に発生する植物マイコプラズマの系統学的解析" 日本マイコプラズマ学会雑誌. 24. 100-102 (1997)
• [Publications] 澤柳利実: "植物マイコプラズマの16S/23S rRNAスペーサー配列を用いた系統学的解析" 日本マイコプラズマ学会雑誌. 24. 103-105 (1997)
• [Publications] 各務 孝: "植物マイコプラズマの病原性と染色体外DNA" 日本マイコプラズマ学会雑誌. 24. 106-108 (1997)
• [Publications] 山本伸一: "植物マイコプラズマのゲノム" 日本マイコプラズマ学会雑誌. 24. 109-110 (1997)
• [Publications] 難波成任: "植物マイコプラズマの系統分類" 日本細菌学雑誌. 52. 印刷中 (1998)