| Project/Area Number |
09470044
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pathological medical chemistry
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| Research Institution | Kagawa Medical University |
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Principal Investigator |
KOBAYASHI Ryoji Kagawa Medical University, Faculty of Medicine, Professor, 医学部, 教授 (00020917)
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| Co-Investigator(Kenkyū-buntansha) |
SUGANUMA Tatsuo Miyazaki Medical College, Faculty of Medicine, Professor, 医学部, 教授 (60115350)
OKABE Akinobu Kagawa Medical University, Faculty of Medicine, Professor, 医学部, 教授 (20093677)
MAETA Hajime Kagawa Medical University, Faculty of Medicine, Professor, 医学部, 教授 (00075508)
前田 肇 香川医科大学, 医学部, 教授 (20294754)
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| Project Period (FY) |
1997 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥8,300,000 (Direct Cost: ¥8,300,000)
Fiscal Year 1998: ¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 1997: ¥5,100,000 (Direct Cost: ¥5,100,000)
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| Keywords | extracellular matrix protein / Ca^<2+> binding protein / Smith-Magenis syndrome / elasith / EF hand protein / blood vessel / microfibril / inhibitor / 36kDa MAGP / Smith-Magenis症候群 / ミクロフィブリン |
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| Research Abstract |
A new extracellular matrix protein of 36 kDa has been purified from bovine and porcine aorta. The protein, 36 kDa microfibril associated glycoprotein (36kDa - MAGP), has a fibrinogen - like domain and contained the sequence Arg - Gly - Asp in the N - terminal region, which is the site for the association with cell and extracellular matrix. Immunoelection microscopy specified its location to elastin - microfibrils.
Using Ca^<2+> - dependent affinity chromatography on an isoquinoline sulfonamide (CKA 1303 ) coupled agarose, we obtained pure form of 36 kDa -MAGP.This compound should serve as a useful tool for clarifying the physiological roles of 36 kDa - MAGP.During the synthesis and selection of S - 100 protein antagonists derived from cinnamic acid and anthranilic acid, we discovered that N - acetyl anthranilic acid and O - (3', 4' - dimethoxy cinnamoyl) salicilic acid strongly bind to 36kDa-MAGP.
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