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Molecular Mechanisms of Redox-Regulation in Viral Pathogenesis

Research Project

Project/Area Number 09470046
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Pathological medical chemistry
Research InstitutionKumamoto University

Principal Investigator

AKAIKE Takaaki  Kumamoto University School of Medicine, Associate Professor, 医学部, 助教授 (20231798)

Co-Investigator(Kenkyū-buntansha) SAWA Tomohiro  Kumamoto University School of Medicine, Research Associate, 医学部, 助手 (30284756)
MAEDA Hiroshi  Kumamoto University School of Medicine, Professor, 医学部, 教授 (90004613)
Project Period (FY) 1997 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥5,800,000 (Direct Cost: ¥5,800,000)
Fiscal Year 1999: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1998: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1997: ¥3,300,000 (Direct Cost: ¥3,300,000)
Keywordsviral pathogenesis / NO / oxidative stress / peroxynitrite / free radicals / viral mutation / viral evolution
Research Abstract

The importance of free radical molecular species in the pathogenesis of various viral diseases has been increasingly recognized in recent years. Oxygen radicals such as superoxide (OィイD3-(/)2ィエD3) and hydroxyl radical (・OH) have been implicated as possible pathogenic molecules in viral disease pathogenesis. Much attention has been given to another simple inorganic radical, i.e., NO in the host's defense mechanism and pathogenesis of virus infection. The NO synthesis pathway, in particular the inducible isoform of NO synthase (iNOS), is expressed in different viral diseases via induction of proinflammatory cytokines such as interferon-γ and interleukin-1β. iNOS produces an excessive amount of NO for a long time compared with other constitutive isoforms of NOS, i.e., neuronal NOS and endothelial NOS. Our present results indicate that overproduction of NO and oxygen radicals should be a common phenomenon in various infections. Reactive nitrogen oxide species such as peroxynitrite are prod … More uced in biological systems through the reaction of NO with OィイD3-(/)2ィエD3. Peroxynitrite and its biological actions are of considerable interest in that peroxynitrite causes oxidation and nitration of amino acid residues of proteins and guanine of DNA, lipid peroxidation, and DNA cleavage. In fact, it is revealed in our current study that peroxynitrite is critically involved in the viral pathogenesis through its nonselective injury to the host'cells and tissues. Thus, understanding of the role of NO and oxygen radical generation in virus infections will provide insight into not only viral pathogenesis but also the host-pathogen interaction in microbial infections at a molecular level. Moreover, among various NO-induced pathological effects, the mutagenic potential of NO in microbial pathogens is also intriguing. For viral mutation, it is of potential interest to investigate a possible association of oxidative stress and virulence of viruses. In this context, our recent study verifies for the first time that oxidative stress induced by high-output NO accelerates RNA virus mutations. Briefly, by using a recombinant RNA virus, Sendai virus (a negative-sense and single-strand RNA virus), containing a marker gene for genetic mutation and iNOS knockout mice, we obtained solid and direct evidence showing that overproduction of NO in the hosts (wild-type mice) in vivo apparently increases and accelerates viral mutation rates compared with the situation in iNOS-deficient mice. This process of accelerated mutation expands the heterogeneity of variants of the pathogen, leading to rapid evolution under selective pressure. NO and OィイD3-(/)2ィエD3- and hence peroxynitrite generation occurs universally in infected hosts. This finding therefore has great implications for the RNA virus evolution in general, including the rapid generation of drug-resistant and immunologically tolerant and cell tropism-altered mutants of HIV in vivo. Less

Report

(4 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • 1997 Annual Research Report
  • Research Products

    (104 results)

All Other

All Publications (104 results)

  • [Publications] H. Muranaka, et al.: "superoxide scavenging activity of erythromycin-iron complex"Biochem. Biophys. Res. Commun.. 232. 183-187 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] D.C. Hooper: "Prevention of experimental allergic encephalitis by targeting nitric oxide and peroxynitrite: Implications for the treatment of multiple sclerosis"Proc. Natl. Acad. Sci. U.S.A.. 94. 2528-2533 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] T. Okamoto, et al.: "Activation of human neutrophil procollagenase ny nitrogen dioxide and peroxynitrite: A novel mechanism for procollagenase activation involving nitric oxide"Arch. Biochem. Biophys.. 342. 261-274 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] K. Umezawa, et al.: "Induction of nitric oxide synthesis and xanthine oxidase and their role in the antimicrobial mechanism againdt Salmonella typhimurium in mice"Infect. Immun.. 65. 2932-2940 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] T. Akaike, et al.: "nanomolar quantification and identification of various mitrosothiols by high performance liquid chromatography coupled with flowreactors of metals and Griess reagent"J. Biochem.. 122. 459-466 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 赤池孝章、他: "NOによる感染防御反応の表裏"血管と内皮('97増刊号). 7. 17-25 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 赤池孝章: "NO合成酵素阻害剤と抗NO物質の化学と薬理"最新医学. 52. 948-954 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 赤池孝章: "ウイルス感染病態とNO"ウイルス. 47. 165-176 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] T. Akaike, et al.: "Free radicals in viral pathogenesis: Molecular mechaniusms involving superoxide and NO"proc. Soc. Exp. Biol. Med.. 217. 64-73 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] J. Wu, et al.: "Modulation of enhanced vascular permeability in tumors by abradykininantagonist, a cyclooxygenase inhibitor, and a nitric oxide scavenger"Cancer Res.. 58. 159-165 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] H. Maeda, et al.: "Human matrix metalloprotease activation by insults of bacterial infection involving proteases and free radicals"Biol. Chem.. 379. 193-200 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] K. Sato, et al.: "Therapeutic effect of erythromycin on on influenza Virus-induced lung injury in mice"Am. J. Respir. Crit. Care Med.. 157. 853-857 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] H. Maeda and T. Akaike: "Nitric oxide and oxygen radicals in infection, inflammation, and cancer"Biochemistry (Moscow). 63. 854-865 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] M. Yoshida, et al.: "the possible effect of nitric oxide on relation and noradrenaline release in the isolated rabbit urethra"Eur. J. Pharmacol.. 357. 213-219 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] M. Yoshida, et al.: "Effct of the NO scavenger carboxy-PTIO on endothelium-dependent vasorelaxation of various blood vessels from rabbits"Life Sciences. 62. 203-211 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 赤池孝章、前田浩: "ウイルス感染病態におけるNOと酵素ラジカルの役割"細胞工学. 17. 210-218 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 赤池孝章: "特集/NO(一般化窒素)NOS阻害剤の臨床応用の可能性"循環器科. 44. 459-466 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] H. Sugiura, et al.: "Role of peroxynitrite in airway microvascular hyperpermeability during late allergic phase in guinea pigs"Am. J. Respir. Crit. Care Med.. 160. 663-671 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] K. Doi, et al.: "Induction of haem oxygenase-1 by nitric oxide and ischaemia in experimental solid tumours and implications for tumour growth"Br. J. Cancer. 80. 1945-1954 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] K.Inoue, et al.: "Nitrosothiol formation catalyzed by ceruloplasmin: Implication for cytoprotective mechanism in vivo"J. Biol. Chem.. 274. 27069-27075 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] S. Fujii, et al.: "Role of nitric oxide in pathogenesis of herpes simplex virus encephalitis in rats"Virology. 256. 203-212 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 赤池孝章: "ウイルス感染とレドックス"呼吸と循環. 47. 127-134 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 赤池孝章、前田浩: "NOの生理活性(1)感染、炎症、免疫とNO"臨床消化器内科. 14. 671-680 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 赤池孝章: "ウイルス性肺炎とパーオキシナイトライト"遺伝子医学. 3. 499-504 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 岡本竜哉、他: "マトリックスメタロプロテアーゼ(MMP)の活性化機構"生化学. 71. 1387-1401 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] T. Akaike, et al.: "Viral mutation accelerated by nitric oxide production during infection in vivo"FASEB J.. (in press). (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] T. Sawa, et al.: "Tumor-targeting chemotherapy by a xanthine oxidase-polymer conjugate that generates oxygen-free radicals in tumor tissue"Cancer Res.. 60. 666-671 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] T. Sawa, et al.: "Tyrosine nitration by peroxynitrite formed from mitric oxide and superoxide generated by xanthine oxidase"J. Biol. Chem.. (in press). (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] H. Kuwahara, et al.: "helicobactor pylori urease suppresses bactericidal activity of peroxynitrite vis carbon dioxide production"Infect Immun.. (in press). (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 赤池孝章: "IV.炎症に関与する物質(室田誠逸、柏崎禎夫編)"(株)医薬ジャーナル社(東京). 11 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 赤池孝章: "Annual Review呼吸器1998(工藤翔ニ、土屋了介、大田健編)"(株)中外医学社(東京). 8 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] T. Akaike and H. Maeda: "Nitric Oxide and Infection (ed. F.C. Fang)"Kluwer Academic/Plenum Publishing Co., New York. 19 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] T. Akaike: "Guanidino Compounds (eds. M. Mori, M. Ishida and J.F. Clark)"Blackwell Science Asia, Tokyo. 5 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 赤池孝章: "NOの多彩な機能に迫る(谷口直之、鈴木敬一郎編)"(株)羊土社(東京). 10 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] T.Akaike and H.Maeda: "Nitric Oxide (ed. L.J. Ignarro)"Academic Press, San Diego (in press). (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] H. Muranaka, et al.: "Superoxide scavenging activity of erythromycin-iron complex."Biochem. Biophys. Res. Commun.. 232. 183-187 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] D.C. Hooper, et al.: "Prevention of experimental allergic encephalitis by targeting nitric oxide and peroxynitrite: Implications for the treatment of multiple sclerosis."Proc. Natl. Acad. Sci. U.S.A.. 94. 2528-2533 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] T. Okamoto, et al.: "Activation of human neutrophil procollagenase by nitrogen dioxide and peroxynitrite: A novel mechanism for procollagenase activation involving nitric oxide."Arch. Biochem. Biophys.. 342. 261-274 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] K. Umezawa, et al.: "Induction of nitric oxide synthesis and xanthine oxidase and their role in the antimicrobial mechanismSalmonella typhimurium in mice."Infect. Immun.. 65. 2932-2940 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] T. Akaike, et al.: "Nanomolar quantification and identification of various nitrosothiols by high performance liquid chromatography coupled with flow reactors of metals and Griess reagent"J. Biochem.. 122. 459-466 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] K. Sato, et al.: "Nitric oxide generation from hydroxyurea via copper-catalyzed peroxidation and implications for pharmacological actions by hydroxyurea."Jpn. J. Cancer Res.. 88. 1199-1204 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] T. Akaike, et al.: "Free radicals in viral pathogenesis: Molecular mechanisms involving superoxide and NO"Proc. Soc. Exp. Biol. Med.. 217. 64-73 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] M. Yoshida, et al.: "Effect of the NO scavenger carboxy-PTIO on endothelium-dependent vasorelaxation of various blood vessels from rabbits."Life Sciences. 62. 203-211 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] J. Wu, et al.: "Modulation of enhanced vascular permeability in tumors by a bradykinin antagonist, a cyclooxygenase inhibitor, and a nitric oxide scavenger"Cancer Res.. 58. 159-165 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] H. Maeda, et al.: "Human matrix metalloprotease activation by insults of bacterial infeciton involving proteases and free radicals."Biol.Chem. 379. 193-200 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] K. Sato, et al.: "Therapeutic effect of erythromycin on influenza virus-induced lung injury in mice"Am. J. Respir. Crit. Care Med.. 157. 853-857 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] H. Maeda and T. Akaike: "Nitric oxide and oxygen radicals in infection, inflammation, and cancer"Biochemist!y (Moscow). 63. 854-865 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] T. Sawa, et al.: "Lipid peroxyl radicals from oxidized oils and heme-iron: Implication of a high-fat diet in colon carcinogenesis"Cancer Epidemiology, Biomarkers & Prevention. 7. 1007-1012 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] M. Yoshida, et al.: "The possible effect of nitric oxide on relaxation and noradrenaline release in the isolated rbbit urethra"Eur. J. Pharmacol.. 357. 213-219 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] S. Fujii, et al.: "Role of nitric oxide in pathogenesis of herpes simplex virus encephalitis in rats"Virology. 256. 203-212 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] H. Sugiura, et al.: "Role of peroxynitrite in airway microvascular hyperpermeability during late allergic phase in guinea pigs"Am. J. Respir. Crit. Care Med.. 160. 663-671 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] K. Doi, et al.: "Induction of haem oxygenase-1 by nitric oxide and ischaemia in experimental solid tumours and implications or tumour growth"Br. J. Cancer. 80. 1945-1954 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] K. Inoue, et al.: "Nitrosothiol formation catalyzed by ceruloplasmin: Implication for cytoprotective mechanism in vivo"J. Biol. Chem.. 274. 27069-27075 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] T. Akaike, et al: "Viral mutation accelerated by nitric oxide production during infection in vivo"FASEB J.. (in press). (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] T. Sawa, et al.: "Tumor-targeting chemotherapy by a xanthine oxidase-polymer conjugate that generates oxygen-free radicals in tumor tissue."Cancer Res.. 60. 666-671 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Y. Miyamoto, et al.: "Novel functions of human αィイD21ィエD2-protease inhibitor after S-nitrosylation: Inhibition of cysteine protease and antibacterial activity"Biochem. Biophys. Res. Commun.. 267. 918-923 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Y. Miyamoto, et al.: "S-Nitrosylated human αィイD21ィエD2-protease inhibitor"Biocim. Biophys. Acta. (in press). (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] T. Sawa, et al.: "Tyrosine nitration by peroxynitrite formed from nitric oxide and superoxide generated by xanthine oxidase."J. Biol. Chem.. (in press). (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] H.Kuwahara, et al.: "Helicobacter pylori urease suppresses bactericidal activity of peroxynitrite via carbon dioxide production"Infect. Immun.. (in press). (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] T. Akaike and H. Maeda: "Nitric oxide in influenza. In Nitric Oxide and Infection (ed. F.C. Fang)"Kluwer Academic/Plenum Publishing Co., New York. 397-415 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] T. Akaike: "Nitric oxide in infectious diseases. In Guanidino Compounds (eds. M. Mori, M. Ishida and J.F.Clark)"Blackwell Science, Asia,Tokyo. 175-179 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] T. Akaike and H. Maeda: "Pathophysiological effects of high-output production of nitric oxide. In Nitric Oxjde (ed. L.J. Ignarro)"Academic Press, San Diego(in press). (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] T. Sawa,et al.: "Alkylperoxyl radical-scavenging activity of various flavonoids and other phenolic compounds: Implications for the anti-promoter effect of vegetables"J. Agric. Food Chem.. 42. 397-402 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] S. Fujii,et al.: "Role of nitric oxide in pathogenesis of herpes simplex virus encephalitis in rats"Virology. 256. 203-212 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] H. Sugiura,et al.: "Role of peroxynitrite in airway microvascular hyperpermeability during late allergic phase in guinea pigs"Am. J. Respir. Crit. Care Med.. 160. 663-671 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] K. Doi,et al.: "Induction of haem oxygenase-1 by nitric oxide and ischaemia in experimental solid tumours and implications for tumour growth"Br. J. Cancer. 80. 1945-1954 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] K. Inoune,et al.: "Nitrosothiol formation catalyzed by ceruloplasmin: Implication for cytoprotective mechanism in vivo"J. Biol. Chem.. 274. 27069-27075 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Y. Suzuki,et al.: "Direct evidence of vivo nitric oxide production and inducible nitric oxide synthase mRNA expression in the brain of living rat during experimental meningitis"J. Cereb. Blood Flow Metab.. 19. 1175-1178 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Y. Miyamoto,et al.: "Novel functions of human α_1-protease inhibitor after S-nitrosylation: Inhibition of cysteine protease and antibacterial activity"Biochem. Biophys. Res. Commun.. 267. 918-923 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Y. Miyamoto,et al.: "S-Nitrosylated human α_1-protease inhibitor"Biochim. Biophys. Acta. (in press). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] T. Akaike,et al.: "Viral mutation accelerated by nitric oxide production during infection in vivo"FASEB J.. (in press). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] T. Akaike and H. Maeda: "Nitric Oxide and Infection (ed. F. C. Fang)"Kluwer Academic/Plenum Publishing Co., New York. 19 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] T.Akaike: "Guanidino Compounds (eds. M. Mori, M. Ishida and J. F. Clark)"Blackwell Science Asia, Tokyo. 5 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] T. Akaike and H. Maeda: "Nitric Oxide (ed. L. J. Ignarro)"Academic Press, San Diego (in press). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Akaike,T.,et al.: "Free radicals in viral pathogenesis:Molecular mechanisms involving superoxide and NO" Proc.Soc.Exp.Biol.Med.217. 64-73 (1998)

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  • [Publications] Yoshida,M.,et al: "Effect of the NO scavenger carboxy-PTIO on endothelium-dependent vasorelaxation of various blood vessels from rabbits" Life Sciences. 62. 203-211 (1998)

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  • [Publications] Wu J.,et al.: " Modulation of enhanced vascular permeability in tumors by a bradykinin antagonist,a cyclooxygenase inhibitor,and a nitric oxide scavenger" Cancer Res.58. 159-165 (1998)

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  • [Publications] Maeda,H.,et al.: "Human matrix metalloprortease activation by insults of bacterial infection involving proteases and free radicals" Biol.Chem.379. 193-200 (1998)

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  • [Publications] Sato,K.,et al.: "Therapeutic effect of eryhromycin on influenza virus-induced lung injury in mice" Am.J.Respir.Crit.Care Med.157. 853-857 (1998)

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  • [Publications] Maeda,H.,and Akaike,T.: "Nitric oxide and oxygen radicals in infection,inflammation,andcancer" Biochemistry(Moscow). 63. 854-865 (1998)

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  • [Publications] Yoshida,M.,et al.: "The possible effect of nitric oxide on relaxation and noradrenaline release in the isolated rabbit urethra" Eur.J.Phamacol.357. 213-219 (1998)

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  • [Publications] Nakatsubo,N.,et al.: "Improved nitric oxide detection usisng 2,3-diaminonaphthalene and its application to the evaluation of novel nitric oxide synthase inhibitors" Biol.Pham.Bull.21. 1247-1250 (1998)

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  • [Publications] Sawa,T.,et al.: "Lipid peroxyl radicals from oxidized oils and heme-iron:Implication of a high-fat diet in colon carcinogenesis" Cancer Epidemiol.Biomarkers & Prevention. 7. 1007-1012 (1998)

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  • [Publications] Doi,K.,et al.: "Inducation of heme oxygenase-1 by nitric oxide and ischemia in experimental solid tumors and implications for tumor growth" Br.J.Cance. (in press). (1999)

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  • [Publications] Fujii,S.,et al.: "Role of nitric oxide in pathogenesis of herpes simplex virus encephalitis in rats" Virology. (in press). (1999)

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  • [Publications] Maeda,H.,et al.: "Free radical generation from heterocyclic amines by cytochrome b5 reductase in the presence of NADH" Cancer Letters. (in press). (1999)

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  • [Publications] Maeda,H.,et al.: "Kallikrein-kinin in infection and cancer" Immunopharmacology. (in press). (1999)

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  • [Publications] Akaike,T.,and Maeda,H.: "Nitric Oxide and Infection(ed.F.C.Fang)" Plenum Publishing Co.,New York(in press), (1999)

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  • [Publications] T. Okamoto, et al.: "Activation of human matrix metalloproteinases by various bacterial proteinases" J. Biol. Chem.272. 6059-6066 (1997)

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  • [Publications] H. Muranaka, et al.: "Superoxide scavenging activity of erythromycin-iron complex" Biochem. Biophys. Res. Commun.232. 183-187 (1997)

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  • [Publications] D.C. Hooper, et al.: "Prevention of experimental allergic encephalitis by targeting nitric oxide and peroxynitrite : Implications for the treatment of multiple sclerosis" Proc. Natl. Acad. Scie. U.S.A. 94. 2528-2533 (1997)

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  • [Publications] K. Umezawa, et al.: "Induction of nitric oxide synthesis and xanthine oxidase and their role in the antimicrobial mechanism against Salmonella typhimurium in mice" Infect. Immun.65. 2932-2940 (1997)

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  • [Publications] K. Sato, et al.: "Nitric oxide generation from hydroxyurea via copper-catalyzed peroxidation and implications for pharmacological actions of hydroxyurea" Jpn. J. Cancer Res.88. 1199-1204 (1997)

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  • [Publications] T. Akaike, et al.: "Free radicals in viral pathogenesis : Molecular mechanisms involving superoxide and NO" Proc. Soc. Exp. Biol. Med.217. 64-73 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] M. Yoshida, et al.: "Effect of the NO scavenger carboxy-PTIO on endothelium-dependent vasorelaxation of various blood vassels from rabbits" Life Sciences. 62. 203-211 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] H. Maeda, et al.: "Human matrix metalloprotease activation by insults of bacterial infection involving proteases and free radicals" Biol. Chem.379. 193-200 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] J. Wu, et al.: "Modulation of enhanced vascular permeability in tumors by a bradykinin antagonist, a cyclooxygenase inhibitor, and a nitric oxide scavenger" Cancer Res.58. 159-165 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] K. Sato, et al.: "Therapeutic effect of erythromycin on influenza virus-induced lung injury in mice." Am. J. Respir. Crit. Care Med.(in press). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] H. Maeda, and T. Akaike: "Nitric oxide and oxygen radicals in infection, inflammation and cancer" Biochemistry (Moscow). (in press). (1998)

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  • [Publications] H. Maeda and T. Akaike: "Nitric Oxiden in Transplant Rejection and Anti-tumor Defense (eds. J.L. Zweier and S.J. Lukiewicz)" Kluwer Academic Publishers, Norwell (in press), (1998)

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  • [Publications] T. Akaike and H. Maeda: "Nitric Oxide and Infection (ed. F.C. Fang)" Plenum Publishing Co., New York (in press), (1998)

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Published: 1997-04-01   Modified: 2016-04-21  

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