| Project/Area Number |
09470052
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| Research Category |
Grant-in-Aid for Scientific Research (B).
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Human pathology
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| Research Institution | KYUSHU UNIVERSITY |
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Principal Investigator |
TSUNEYOSHI Masazumi Faculty of Med., KYUSHU UNIVERSITY, Prof., 大学院・医学研究院, 教授 (20091259)
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| Co-Investigator(Kenkyū-buntansha) |
HAYASHI Kenshi Faculty of Med., KYUSHU UNIVERSITY, Prof., 遺伝実験情報施設, 教授 (00019671)
TAMIYA Sadafumi School of Health Sciences, KYUSHU UNIVERSITY, Ass.Prof., 医療短期大学, 講師 (60284486)
ODA Yoshinao Faculty of Med., KYUSHU UNIVERSITY, Ass.Prof., 大学院・医学研究院, 講師 (70291515)
FUKUDA Toshiro Funuoka Red Cross Hospital, Chief, 部長(研究職)
KAWAUCHI Shigeto Yamaguchi Univ.Fac.of Med., Ass.Prof., 医学部・第二病理, 講師
八反田 洋一 九州がんセンター, 部長(研究職)
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| Project Period (FY) |
1997 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥13,600,000 (Direct Cost: ¥13,600,000)
Fiscal Year 2000: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1999: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1998: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1997: ¥11,100,000 (Direct Cost: ¥11,100,000)
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| Keywords | bone / soft tissue / sarcoma / metastasis / tumor progression / pathology / molecular biology / growth factor / 軟部デスモイド腫瘍 / β-catenin / cyclin D1 / APC遺伝子 / ABI PRISM 7700 / HGF / C-MET / 平滑筋肉腫 / P53 / MDM2 / 癌関連遺伝子 / 増殖因子 / 骨軟部肉腫 |
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| Research Abstract |
We assessed the biological character of bone and soft tissue tumors by molecular biology technique in addition to clinico-pathologic analysis.
1. Malignant transformation : 1) Neurofibroma to malignant peripheral nerve sheath tumor ; abnormal expression of p53 protein, 2) Dermatofibrosarcoma protuberans to fibrosarcoma ; chromosomal abnormality.
2. Dedifferentiated chondrosarcoma and dedifferentiated liposarcoma characterized by high grade malignancy : H-ras mutation
3. Biological behavior in sarcomas
1) Leiomyosarcoma : p53 mutation in 4 dead cases among favorable superficial type. Using Real-Time Semi-quantitative RT-PCR, an increasing level of p53 mRNA associated with an overexpression of p53 protein. 2) Synovial sarcoma : Unfavorable prognostic factors (i) high AJC stage, (ii) large tumor size, (iii) co-expression of HGF/c-MET, (iv) mutation of p53, MDM2, H-ras, beta-catenin. 3) Malignant rhabdoid tumor (i) Immunoexpression of cytokeratin 8 and 18, (ii) Subcellular distribution of cytokeratin and vimentin in rhabdoid cell by three-dimensional imaging, (iii) Genomic abnormality of vimentin and cytokeratin, (iv) Unfavorable prognostic factors : abnormal expression of beta-catenin, p53, hSNF5/INI1 4)Osteosarcoma : Significant nm23, c-MET in metastasis and tumor progression.
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