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Analysis of aberrantly expressed glycoproteins in human digestive tract

Research Project

Project/Area Number 09470053
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Human pathology
Research InstitutionKagoshima University

Principal Investigator

SATO Eiichi  Faculty of Medicine, Kagoshima University, Professor, 医学部, 教授 (60004579)

Co-Investigator(Kenkyū-buntansha) MATSUSHITA Yoshifumi  Faculty of Medicine, Kagoshima University, Research Associate, 医学部, 助手 (90244227)
YONEZAWA Suguru  Faculty of Medicine, Kagoshima University, Associate Professor, 医学部, 助教授 (10175002)
OZAWA Masayuki  Faculty of Medicine, Kagoshima University, Professor, 医学部, 教授 (90136854)
AIKOU Takashi  Faculty of Medicine, Kagoshima University, Professor, 医学部, 教授 (60117471)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥12,600,000 (Direct Cost: ¥12,600,000)
Fiscal Year 1998: ¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 1997: ¥8,900,000 (Direct Cost: ¥8,900,000)
KeywordsDigestive tract / Mucin / Glycoprotein / MUC1 / Sialyl Lewis X / Stomach / Hepato-biliary tract / Colon / MDC1 / シリアル・ルイス・X / 消化器癌 / トロンボモジュリン
Research Abstract

Esophagus : We have examined the expression of thrombomodulin (TM), a glycoprotein having lectin-like domain and possible candidate for adhesion molecule, on the esophageal carcinoma cells. We used variant esophageal carcinoma cell lines that express different amount of TM of their cell surfaces. The TM-low-expressing esophageal carcinoma cells express high invasiveness than to TM-high-expressing carcinoma cells in matrigel invasion assay.
Stomach : In gastric carcinomas, we have examined the expression of MUG-1 and MUC-2 apomucins. The cases of MUC-l(+), MUC-2(-) gastric cancers revealed poor prognosis compared to the cases of MUC-1(-), MUC-2(+).
Liver, Biliary Tract, Pancreas : In these areas, there are two types of tumors, invasive type and expansive type. The former express significantly much amount of MUG-I antigen than to the latter. In contrast, MUC-2 antigen is expressed mainly in the latter but rarely in the former. The prognosis of these cases are also related to the expression pattern of MUC-1 and MUC-2 apomucins.
Colo-rectum : We have performed Stamper-Woodruff assay to examine the adhesion between colon tumor cells and human liver. The resected normal part of the liver of non-hepatitis -virus-infected case was infused with IL-1beta. After confirming the expression of E- and/or P-selectins, colon cancer cells expressing different amount of sialyl-Lewis x antigen were loaded and adhered. Much amount of SLX-high-expressing tumor cells adhered compared to SLX-bow-expressing cells. And this manner is blocked by anti-E-selectin, or anti-SLX antibodies.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] Yoshifumi Matsushita etal: "A subcloned human esophageal squamers all carcinoma cell line with low-thoombomodulin-expression showed increased invasiveness compared with a high-thoombomodubn-expressive clone" Cancer Letters. 127. 195-201 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tamiharu Utsunomiya et al: "Expression of MUC1 and MUC2 mucins in gastric carcinomas : Ets relationship with the prognosis of the patrents" Clinical Cancer Research. 4. 2605-2614 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Hideie Sakamoto et al: "Mucin Antigen expression in gastric caranoma of young and old adults" Human Pathology. 28. 1056-1065 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Suguru Yonezawa et al: "MUC1 mucin expression in invasive areas of intraductel papillary mucinous tumor of the pancreas" Pathology International. 48. 319-322 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Suguru Yonezawa: "Gene expression of gastric type mucin(MUC5AC)in panereatic humors : Its relationship with biological behavior of the tumors" Pathology International. 49. 45-54 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yoshifumi Matsushita et al: "Selectins induced by Interleukin-Iβon the human liver endothelial cells act as lizards for siolyl-Lewis X expressing human colon carcinoma cell metastasis" Cancer Letters. 133. 151-160 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yoshifumi Matsushita et al.: "A subcloned human esophageal squamous cell carcinoma cell line with low-thrombomodulin expression showed increased invasivenes compared with a high-thrombomodulin-expressing clone - thrombomodulin as a possible candidate for an adhesion molecule of squamous cell carcinoma." Cancer Letters. Vol 127. 195-201 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tamiharu Utsunomiya et al.: "Expression of MUC1 and MUC2 mucins in gastric carcinomas : Its relationship with the prognosis of the patients." Clinical Cancer Research. Vol.4. 2605-2614 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Hideie Sakamoto et al.: "Mucin antigen expression in gastric carcinomas of young and old adults." Human Pathology. Vol.28. 1056-1065 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Suguru Yonezawa et al.: "MUC1 mucin expression in invasive areas of intraductal papillary mucinous tumors of the pancreas" Pathology International. Vol.48. 319-322 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Suguru Yonezawa et al.: "Gene expression of gastric type mucin (MUC5AC) in pancreatic tumours : Its relationship with biological behavior of the tumors" Pathology International. Vol.49. 45-54 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yoshifumi Matsushita et al.: "Selectins induced by Interleukin-1beta on the human liver endothelial cells act as ligands for sialyl-Lewis X expressing human colon carcinoma cell metastasis" Cancer Letters. Vol.33. 151-160 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yoshifumi Matsushita etal.: "A Subclored human esophagesl sguamous cell corancma cell line with low thrombonodnlin expression shored increaued invasiveness compared with a high thrombomethulin-expressing clone" Cancer Latters. 127. 195-201 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Tamihara Utsunomiya etal.: "Expression of MVC1 and MVC2 mucins in gastrie carainomas:Its relationship with the prognosion of the patients" Clivical Cancer Research. 4. 2605-2614 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Hideie Sakamoto et al.: "Mucin antigen expression in gastric corainomas of young and old adutls" Human Pathology. 28. 1056-1065 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] Suguru Yomezawa et al.: "MVC-1mucin expression in invasive areas of intiaductal papillary muinous timors of the pancreas" Pathology International. 48. 319-322 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Suguru Yonezawa et al.: "Gene expression of gastnic typemucin(MVC5AC)in prncreath tumors:Its relationship inth biological behavior of the tumors" Pathology International. 49. 45-54 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Yoshifumi Matsushita etal.: "Selectins induced by interleukin-1β on the human liver erdothelial cells act as ligand for sialyl-lewis X expressing human colon carainoma cell metastusis" Cancer Letlers. 133. 151-160 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Sakamoto H,et al.: "Mucin antigens expression in gastric carcinomas of young and old adults" Human Pathology. 28. 1056-1065 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Matsushita Y,et al.: "Carcohydrate antigens aberrantly expressed in colorectal carcinoma" Critical Reviews in ONCOLOGY/HEMTOLOGY. 25. 27-54 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yonezawa S,et al.: "Expression of mucin antigens in human cancers and its relationship with malignant potential" Pathology International. 47. 813-830 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yonezawa S,et al.: "MUC1 mucin expression in invasive areas of intraductal papillary mucinous tumors of the pancreas" Pathology International. (in press).

    • Related Report
      1997 Annual Research Report
  • [Publications] 松下能文、他(分担): "[続]癌の浸潤・転移研究マニュアル-実験の手技と指針 糖鎖抗原の免疫組織学的検出" がん転移研究会(金芳堂), 4 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 松下能文(分担): "[続]癌の浸潤・転移研究マニュアル-実験の手技と指針 CEAならびにCEA関連構造の免疫組織化学的・生化学的検出" がん転移研究会(金芳堂), 4 (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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