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T Cell Response with Stress Proteins

Research Project

Project/Area Number 09470067
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Experimental pathology
Research InstitutionSapporo Medical University

Principal Investigator

SATO Noriyuki  Sch of Med, Sapporo Medical University, 医学部, 教授 (50158937)

Co-Investigator(Kenkyū-buntansha) 菊地 浩吉  札幌医科大学, 医学部, 教授 (00045345)
Project Period (FY) 1997 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥12,800,000 (Direct Cost: ¥12,800,000)
Fiscal Year 1999: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1998: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1997: ¥9,700,000 (Direct Cost: ¥9,700,000)
Keywordshsp70 / molecular chaperones / T cells / antigenic peptides / polyamines / MHC class I / TAP / rat TCR Vδ6 / HSP70 / TCRγδ / 細胞表面発現 / 単クローン抗体 / hsp 70 / TCR / γδ T cell / ハイブリドーマ
Research Abstract

Our study indicated the important role of the 70 kA heat shock proteins (hsp70) for feeding the major histocompatibility antigen complexes (MHC) class I-bound antigenic peptides to the transporters associated with the antigen processing (TAP), and revealed a novel possibility that the repertoire of MHC class I-bound peptides may be limited, at least in part, in the cytosol by their affinity to molecular chaperone hsp70. We also indicated an involvement of polyamines in the interaction between hsp70 and TAP, suggesting the possible role of polyamines in the tumor escape mechanisms.
It was also suggested that some form of hsp70 is expressed on the cell surface with as yet unknown mechanism. Our current data support the notion that an hsp70-like molecule could be presented together with the cellular peptides antigens to particular T cells with the T-cell receptor (TCR) γδ chains. Since the expression of this hsp70-like molecule on the cell surface is usually induced along with cell transformation by activated oncogenes, it is likely that T cells with the TCR Vδ6 family contribute to the host's resistance to tumor cells.

Report

(4 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • 1997 Annual Research Report
  • Research Products

    (40 results)

All Other

All Publications (40 results)

  • [Publications] Kamuguchi, K., Torigoe, T., Fujiwara, O., Hirohashi, Y., Sahara, H., I, Sato, N.: "73 kD heat shock cognate protein is associated with transporters associated with antigen processing (TAP) and involved in feeding of MHV vlass I-presentable antigenic peptides to TAP."J. Exp. Med.. in press.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Hatamoto, K., Torigoe, T., Mano, Y., Asai, Y., Sato, N.: "Transduction of TAT fusion protein disrupts the interaction between retinoblastoma protein and 70 kD heat shock protein in cells, leading to growth acceleration"Cell Growth Different. in press.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Matsuura, A., Kinebuchi, M., Kikuchi, K., Sato, N., et al.: "NK T cell in the rat : organ-specific distribution on NK T cells expressing distinct Val4 chains"J. Immunol. , March,2000. (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Nagata, M., Akazawa, T., Tamura, Y., Sagae, S., Kudo, R., Sato, N. et al: "The expression of a novel natural killer inhibitory molecule, Cho-1, on the chorionic cytotrophoblast cells of successful pregnancy, but not of spontaneous abortion"Clin. Exp. Immunol.. in press.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Maeda, A., Ohguro, H., Maeda, T., Sato, N., Kuroki, Y., Nakagawa, T. et al.: "Aberrant expression of photoreceptor specific calcium binding protein (recoverin) in cancer cell lines"Cancer. Res. , April, 2000. (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Suzuki, K., Sahara, H., Okada, Y., Hirata, K., Kikuchi, K., Sato, N., et al: "Identification of natural antigenic peptides of a human gastric signet ring cell caricinoma recognized by HLA-A31-restricted cytotoic T lymphocytes"J. Immunol.. 163. 2783-2791 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Sato, N., Okada, Y., Suzuki, K., Wada, Y., Hirata, K., Kikuchi, K., et al: "Gann Monogro. Jap. J. Cancer Res."Natural antigenic peptides of gastric signet ring cell carcinomas. 10 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kikuchi, K., and Sato, N.: "Gann monogr. Jap. J. Cancer Res."Cell-mediated Immunity to autologous cancer. Rationale of immunotherapy of human cancer with CTL.. 12 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kamuguchi,K., Torigoe,T., Fujiwara,O., Hirohashi,Y., Sahara,H., Hiraii,I., Sato,N.: "73 kD heat shock cognate protein is associated with transporters associated with antigen processing (TAP) and involved in feeding of MHV vlass I-presentable antigenic peptides to TAP"J. Exp. Med.. in press.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Hatamoto,K., Torigoe,T., Mano,Y., Asai,Y., Sato,N.: "Transduction of TAT fusion protein disrupts the interaction between retinoblastoma protein and 70 kD heat shock protein in cells, leading to growth acceleration."Cell Growth Different. in press.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Matsuura,A., Kinebuchi,M., Kikuchi,K., Sato,N.: "NK T cells in the rat: organ-specific distribution on NK T cells expressing distinct Va14 chains."J. Immunol.. (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Nagata,M., Akazawa,T., Tamura,T., Sagae,S., Kudo,R., Sato,N.: "The expression of a novel natural killer inhibitory molecule, Cho-1, on the chorionic cytotrophoblast cells of successful pregnancy, but not of spontaneous abortion."Clin. Exp. Immunol.. in press.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Maeda,A., Ohguro,H., Maeda,T., Sato,N., Kuroki,Y., Nakagawa,T.: "Aberrant expression of photoreceptor specific calcium binding protein (recoverin) in cancer cell lines."Cancer. Res.,. (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Suzuki,K., Sahara,H., Okada,Y., Hirata,K., Kikuchi,K., Sato,N.: "Identification of natural antigenic peptides of a human gastric signet ring cell carcinoma recognized by HLA-A31-restricted cytotoic T lymphocytes."J. Immunol.. 163. 2783-2791 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Sato,N., Okada,Y., Suzuki,K., Wada,Y., Hirata,K., Kikuchi,K.: "Natural antigenic peptides of gastric signet ring cell carcinomas"Gann Monogr. Jap. J. Cancer Res.. 48. 31-41 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kikuchi,K., Sato,N.: "Cell-mediated Immunity to autologous cancer. Rationale of immunotherapy of human cancer with CTL."Gann Monogr. Jap. J. Cancer Res.. 48. 3-14 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kamuguchi,K.,Torigoe,T.,Fujiwara,O.,Hirohashi,Y.,Sahara,H.,Hiraii,I.,Sato,N.: "73 kD heat shock cognate protein is associated with transporters associated with antigen processing (TAP) and involved in feeding of MHV vlass I-presentable antigenic peptides to TAP."J.Exp.Med.. (in press).

    • Related Report
      1999 Annual Research Report
  • [Publications] Hatamoto,K.,Torigoe,T.,Mano,Y.,Asai,Y.,Sato,N.: "Transduction of TAT fusion protein disrupts the interaction between retinoblastoma protein and 70 kD heat shock protein in cells,leading to growth acceleration."Cell Growth Different. (in press).

    • Related Report
      1999 Annual Research Report
  • [Publications] Matsuura,A.,Kinebuchi,M.,Kikuchi,K.,Sato,N.et al.: "NK T cells in the rat: organ-specific distribution on NK T cells expressing distinct Va14 chains."J.Immunol.,March,2000. (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Nagata,M.,Akazawa,T.,Tamura,Y.,Sagae,S.,Kudo,R.,Sato,N.et al: "The expression of a novel natural killer inhibitory molecule,Cho-1,on the chorionic cytotrophoblast cells of successful pregnancy,but not of spontaneous abortion."Clin.Exp.Immunol.. (in press).

    • Related Report
      1999 Annual Research Report
  • [Publications] Maeda,A.,Ohguro,H.,Maeda,T.,Sato,N.,Kuroki,Y.,Nakagawa,T.,et al.: "Aberrant expression of photoreceptor specific calcium binding protein (recoverin) in cancer cell lines."Cancer.Res.,April,2000. (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Suzuki,K.,Sahara,H.,Okada,Y.,Hirata,K.,Kikuchi,K.,Sato,N.et al: "Identification of natural antigenic peptides of a human gastric signet ring cell carcinoma recognized by HLA-A31-restricted cytotoic T lymphocytes."J.Immunol.. 163. 2783-2791 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Sato,N.,Okada,Y.,Suzuki,K.,Wada,Y.,Hirata,K.,Kikuchi,K.et al: "Gann Monogr.Jap.J.Cancer Res."Natural anitigenic peptides of gastric signet ring cell carcinomas.. 10 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Kikuchi,K.,and Sato,N.: "Gann Monogr.Jap.J.Cancer Res."Cell-mediated Immunity to autologous cancer.Rationale of immunotherapy of human cancer with CTL.. 12 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Sogahata,K, Torigoe,T., Ishikawa,M., Kikuchi,K, Sato,N.et al: "Sensitivity to proteasome-mediated degration of dephosphorylated reteinoblastoma protein is regulated by molecular chaperome, HSC73 and HSP40 in vitro." J.Biol.Chem.in press.

    • Related Report
      1998 Annual Research Report
  • [Publications] Akazawa,T., Torigoe,T., Nagasawa,S., Tamura,Y., Sato,N.et al.: "A novel negative regular Cho-1 molecule is involved in the cytotoxicity by human natural killer cells nut not in cytotoxicity T lymphocytes." Microbiol.Immunol.in press.

    • Related Report
      1998 Annual Research Report
  • [Publications] Hirai,J., Sato,N., Qi,W., Ohtani,S., Torigoe,T.and Kikuchi,K.: "Localization of pNT22 70kDa heat shock cognate-like protein in the plasma membrane." Cell Struct.Function.23. 153-158 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Tsuruma,T., Yagihashi,A., Torigoe,T., Sato, N., Hirata,K et al.: "Interleukin-10 reduces natural killer sensitivity and downregulates MHC class I expression on H-ras-transformed cells." Cell.Immunol.184. 121-128 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Sato,N., Okada,Y., Hirata,K., Kikuchi,K.et al.: "Natural antigenic peptides of gastric signet ring cell carcinomas. In “Recent Advances of Human Tumor Immunology and Immunotherapy Edts.K.Kikuchi and B.van den Eynde."" Gann Monogr.Jap.J.Cancer Res.in press.

    • Related Report
      1998 Annual Research Report
  • [Publications] Kikuchi,K.and Sato,N.: "Cell-mediated Immunity to autologous cancer. Rationale of immunotherapy of human cancer with CTL.Gann Monogr.Japa J.Cancer Res., In “Recent Advances of Human Tumor Immunology and Immunotherapy" Edts.K.Kikuchi and B.van den Eynde." Gann Monogr.Jap.J.Caner Res.in press.

    • Related Report
      1998 Annual Research Report
  • [Publications] 平井到、佐藤昇志: "生体反応の分子情報学 中外医学社" 熱ショック蛋白 上出利光、小林邦彦編(印刷中),

    • Related Report
      1998 Annual Research Report
  • [Publications] Sato,N. and Torigoe,T.: "Annals of New York Academy of Sciences" The molecular chaperones in the cell cycle control. In : Stress of life from molecules to man. ed. : P.Csermely, 6 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Takashima,S.,Sato,N.,Kikuchi,K.et al: "Involvement of peptide antigens in the cytotoxicity between 70 kDa heat shock cognate protein-like molecule and CD3^+,CD4^-,CD8^-,T cell receptor α/β^-killer T cells." J.Immunol.157. 3391-3395 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Fujita,T.,Takahashi,S.,Sato,N.et al: "Prolonged survival of rat skin allograft by treatment with FK506 ointment." Transplantation. 64. 922-925 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Miyazaki,A.,Sato,N.,Kikuchi,K.et al: "Cytotoxicity of histocompatibility leukocyte antigen-DR8-restricted CD4_+ killer T cells against human autologous squamous cell carcinoma." Jpn.J.Cancer Res.88. 191-197 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Tsuruma,T.,Yagihashi,A.,Torigoe,T.,Sato,N.,Kikuchi,K and Hirata,K.: "Interleukin-10 reduces natural killer sensitivity of tumor cells." Cell.Immunol.(in press).

    • Related Report
      1997 Annual Research Report
  • [Publications] Kamiguchi,K.,Torigoe,T.,Sato,N.,Kikuchi,K.et al: "73kD heat shock cognate protein is associated with transporter associated with antigen processing." Int.Immunol.(in press).

    • Related Report
      1997 Annual Research Report
  • [Publications] Hirai,I.,Sato,N.,Kikuchi,K.et al: "Localization of pNT22 70kDa heat shock cognate-like protein in the plasma membrane." Exp.Cell Res.(submitted).

    • Related Report
      1997 Annual Research Report
  • [Publications] 佐原弘益、佐藤昇志、高橋延昭、菊地浩吉: "癌拒絶抗体ペプチドによる治療" 中外医学社, 216-225 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Sato,N.,Torigoe,T.: "The molecular chaperones in the cell cycle control.In:Stress of life from molecules to man.ed.:P.Csermely,Annals of New York Academy of Sciences,Vol.851," (in press), (1998)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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