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Genetic susceprtibity to acute or chronic schistosomiasis japonica

Research Project

Project/Area Number 09470071
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 寄生虫学(含医用動物学)
Research InstitutionSaitama Medical School

Principal Investigator

HIRAYAMA Kenji  Professor, Dept of Medical Zoology, Saitama Medical School, 医学部, 教授 (60189868)

Co-Investigator(Kenkyū-buntansha) SATOH Masao  Dept of Medical Zoology, Saitama Medical School, 医学部, 助手 (40255120)
SONE Toshio  Dept of Medical Zoology, Saitama Medical School, 医学部, 講師 (20281743)
Project Period (FY) 1997 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥13,000,000 (Direct Cost: ¥13,000,000)
Fiscal Year 1999: ¥2,700,000 (Direct Cost: ¥2,700,000)
Fiscal Year 1998: ¥4,800,000 (Direct Cost: ¥4,800,000)
Fiscal Year 1997: ¥5,500,000 (Direct Cost: ¥5,500,000)
KeywordsSchistosona japonicum / Major antigen / inmunogenetics / cDNA library / China / Protective immunity / vaccine / pig / 日本住血吸虫 / 主要抗原 / 免疫遺伝学 / cDNAライブラリー / 中国 / 感染防御 / ワクチン / ブタ / 日本住吸血虫 / 肝線維症 / HLA / 超音波診断 / ELISPOT法 / 再感染抵抗性 / 高リスク集団 / サイトカイン / 集団治療
Research Abstract

1.Molecular cloning of the major antigens from a cDNA library of schistosomal egg by using patients pooled sera.
We have already found there are relatively resistant and susceptible HLA-class II haplotypes present in the Chinese patients against post-schistosomal liver fibrosis. This 'ear we seeked for the major antigens that would stimulate those HLA haplotypes positive individuals' T cells, because T cell response is assumed to be the most important in the pathogenesis. A cDNA library was prepared from a mRNA extracted from the freshly isolated eggs of a Chinese strain using lambda gt11 I phage system and was screened for the major antigens reactive with pooled sera by IgG detection system. Finally 200 positive clones were isolated and 146 clones out of those were directly sequenced. The result was unexpected because all the clones were several parts of one gene named miracidial antigen. The cloned gene was successfully expressed by GST fusion protein system.
2. Re-infection study in t … More hree different villages.
We have found two things. One is that there was no age dependent resistance in the population and the other is that there were two categories of people present, resistant and susceptible against re-infection. We have found that the HLA-DRB1 types that resistant persons had shared its 67th amino acid, phenylalanine, instead of having 67 leucine or isoleucine. Because the 67F or I/L is important to make a binding pocket, it would be critical to provoke some protective immune response.
3. Vaccination trial of domestic pigs with recombinant paramyosin in China.
In the endemic area in China, it is important to control the infection of the reservoir hosts, such as pigs and water buffaloes. We have performed a vaccine trial study using domestic pigs and recombinant paramyosin. The extensive study using more than 80 pigs revealed that the pigs were susceptible to challenge infection and were provoked immunity against infection after the vaccination with paramyosin as well as with attenuated cercariae (Vaccine in Press). Less

Report

(4 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • 1997 Annual Research Report
  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] Honggen Chen: "Vaccination of domestic pig with recombinant paramyosin against Schistosoma Japonicum in China"Vaccine. 2000.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 平山謙二: "寄生虫感染の重症化に関する遺伝子"医学のあゆみ. 191(1). 124-130 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Hongg Chen.: "Vaccination of domestic pig with recombinant paramyosin against Schistosoma Japonicum in China."Vaccine. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Honggen Chen: "Vaccination of domestic pig with recombinant Paramyosin against Schistosoma Japonicum in China"Vaccine. (in press). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] 平山謙二: "寄生虫感染の重症化に関与する遺伝子"医学のあゆみ. 191(1). 124-130 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Kenji Hirayama et.al.: "HLA-DR-DQ alleles and HLA-DP alleles are independently associated with susceptibility to different stages of post-schistosomal hepatic fibrosis in the Chinese population." Tissue antigens.in press. (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] 太田伸生: "中国湖南省の日本住血吸虫病調査;揚子江流域の1村落をモデルとして" 熱帯. 30. 93-99 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Kenji Hirayama: "Glycine-Valine Dimorphism at tht 86th Amino Acid of HLA-DRB1 Influenced the Prognosis of Postschistosomal Hepatic Fibrosis" Journal of Infect. 13. 17-29 (1998)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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