| Project/Area Number |
09470073
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bacteriology (including Mycology)
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| Research Institution | Chiba Unibersity |
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Principal Investigator |
NODA Masatoshi Chiba Unibersity, School of Medicine, Chairman snd Professor, 医学部, 教授 (60164703)
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| Co-Investigator(Kenkyū-buntansha) |
MIYAKE Masami Chiba University, School of Medicine, Research Assistant, 医学部, 助手 (10251175)
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| Project Period (FY) |
1997 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥4,000,000 (Direct Cost: ¥4,000,000)
Fiscal Year 1998: ¥4,000,000 (Direct Cost: ¥4,000,000)
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| Keywords | Shiga-like(Vero)toxin / virulence factor / O157 : H7 / Vero cells / lethal toxicity / fluid accumulation / rabbit ileal loop / 白子蛋白 / O157:H7 / O157 / Shiga-like toxin / 抗VT活性 / 大黄 / 桂皮 |
|---|
| Research Abstract |
Shiga-like (Vero) toxin, a heterooligomeric protein composed of one A and five B subunit, is a major virulence factor produced by enterohemorrhagic Escherichia coli O157 : H7. The severe bloody diarrhea observed in E.coli 0157 patients is mediated by Shiga-like toxin- catalyzed cleavage of N-glycosidic bond of the adenosine at 4,324 from the 5'-terminal of the 28S ribosomal RNA of 60S ribosomal subunit in the target cells. We have investigated inhibitors of Shiga-like toxin, of natura1 origin, and found a candidate that inhibits the cytotoxicity of the toxin to Vero cells and a certain line of HeLa cells, lethal toxicity to mice and other small experimental animals, and bloody fluid accumulation in the rabbit ileal loop.
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