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Investigation on the Interaction between the Molecules Constituting Viruses and Application to Gene Therapy

Research Project

Project/Area Number 09470082
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Virology
Research InstitutionTOKYO INSTITUTE OF TECHNOLOGY

Principal Investigator

HANDA Hiroshi  Tokyo Institute of Technology, Frontier Collaborative Research Center, Professor, フロンティア創造共同研究センター, 教授 (80107432)

Co-Investigator(Kenkyū-buntansha) ARISAKA Fumio  Tokyo Institute of Technology, Faculty of Bio sciences and Biotechnology, Associ, 生命理工学部, 助教授 (80133768)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥11,800,000 (Direct Cost: ¥11,800,000)
Fiscal Year 1998: ¥5,200,000 (Direct Cost: ¥5,200,000)
Fiscal Year 1997: ¥6,600,000 (Direct Cost: ¥6,600,000)
KeywordsGene therapy / Molecular recognition / Self-assembly / Cellular directivity / Virus component / Capsid protein / Virus-like particules / Virus vector
Research Abstract

It became possible to make viruses which have been hazardous for the human into useful components by the development of gene-technology and basic studies on virology. Recombinant virus vector has been found much worthy in gene transfer method for maintaining the long term growth of mammalian cells in vitro. A SV40-adenovirus recombinant vector was used immortalize the human bone marrow stromal cells. The chimeric virus vector was found more efficient than DNA transfection method. AAV-2, consists of three capsid proteins at a ratio of VP1 : VP2 : VP3=l0 : 1 : 1. AAV-2 is supported to encapsidate the genome into a pre-formed pro-capsid. Characterization of self assembled capsid as well the mechanism of the assembly of the capsid deserve much importance in studying viral capsid for in vitro system. VLP has been purified from insect cells by infecting with recombinant bacurovirus, containing VP2 coding region alone or co-infection. Thus, various factors participating in VLP formation by self assembly and in partial dissociation and re-assembly of VLP components were elucidated. Accordingly, basic study systems of the application of the recombinant virus capsid protein to gene therapy by using vector of novel genes has been established in this study.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (33 results)

All Other

All Publications (33 results)

  • [Publications] Wada, T., et al.: "DSIF, a novel negative transcription elongation factor that regulates RNA polymerase II processivity, is composed of Human Spt4ad Spt5homologs" Genes Development. 12. 343-356 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yamaguchi, Y., et al.: "Casein kinase II interacts with the bZIP domains of several transcription factors." Nucleic Acids Res.26. 3854-3861 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Suzuki, F., et al.: "Functional Interactions of transcrioption factor hGABP subunits." J.Biol.Chem.273. 29302-29308 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Wada, T., et al.: "Evidence that p-TEFb alleviates the negative effect of DSIF on RNA polymerase II-dependent transcription in vitro." ENBO J.17. 7395-7403 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yamaguchi, Y., et al.: "Structure andfunction of the human transcription elongation factor DSIF." J.Biol.Chem.(1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Sano, Y., et al.: "CBP alleviates the intramolecular inhibition of ATF-2 function." J.Biol.Chem.273. 29098-29105 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kawasaki, H., et al.: "Distinct roles of the co-activators p300 and CBP in retinoic acid-induced F9 cells" Nature. 393. 284-289 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Handa.H., et al.: "DNA binding proteins. In High Resolution Choromatography" Oxford University Press., (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 半田宏. 他: "RNA構造とスプライシング細胞機能と代謝マップ" 東京化学同人, (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Hiramoto, M., Shimizu, N., Sugimoto, K., Tang, J., Kawakami, Y., Ito, M., Aizawa, S., Tanaka, H., Makino, I.and Handa, H.: "Nuclear targetted suppression of NF-kappaB activity by the novel quinone derivative E3330." J.Immunol.160. 810-819 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Hiramoto, M., Aizawa, S., Iwase, O., Nakano, M., Toyama, K., Hoque, M., Nabeshima, R., Kaidow, A., Imai, T., Hoshi, H.and Handa, H.: "Stimulatory effects of substance P on CD34 positive cell proliferation and differentiation in vitro are mediated by the modulation of stromal cell function." Int.J.Mol.Med.1. 347-354 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Wada, T., Takagi, T., Yamaguchi, Y., Ferdous, A., Imai, T., Hirose, S., Sugimoto, S., Yano, K., Hartzog, G.A., Winston, .F., Buratowski, S., and Handa, H.: "SIF,a novel negative transcription elongation factor that regulates RNA polymerase II processivity, is composed of human Spt4 and Spt5 homologs." Genes Dev.12. 343-356 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Hartzog, G.A., Wada, T., Handa, H.and Winston, F.: "Evidence that Spt4, Spt5, and Spt6 control transcription elongation by RNA polymerase II in Saccharomyces cerevisiae." Genes Dev.12. 357-369 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Hirano, F., Tanaka, H., Hirano, Y., Hiramoto, M., Handa, H.Makino, I.and Scheidereit, C.: "Functional interference of sp1 and NF-kappaB through the same DNA binding site." Mol.Cell.Biol.18. 1266-1274 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yamaguchi, Y., Wada, T.and Handa, H.: "Drawing a new view of DRB : Interplay between positive and negative elongation factors." Genes Cells. 3. 9-15 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Vassias, I., Hazan, U., Michel, Y., Sawa, C., Handa, H., Gouya, L.and Morinet, F.: "hGABP transcription factor regulates expression of the human B19 parvovirus promoter." J.Biol.Chem.273. 8287-8293 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Wada,T.,et al.: "DSIF,a novel negativetranscription elongation factor that regulates RNA polymerase II processivity,is composed of Human Spt4 and Spt5 hoachys" Genes Development. 12. 343-356 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Yamaguchi,Y.,et al.: "Casein kinase II interacts with the bZIP domains of several transcription factors." Nucleic Acids Res.26. 3854-3861 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Suzuki,F.,et al.: "Functional Interactions of transcrioption factor hGABP subunits." J.Biol.Chem.273. 29302-29308 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Wada,T.,et al.: "Evidence that p-TEFb alleviates the negative effect of DSIF on RNA polymerase II-dependent transcription in vitro." ENBO J.17. 7395-7403 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Yamaguchi,Y.,et al.: "Structure and function of the human transcription elongation factor DSIF." J.Biol.Chem.(1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Sano,Y.,et al.: "CBP alleviates the intramolecular inhibition of ATF-2 function." J.Biol.Chem.273. 29098-29105 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kawasaki,H.,et al.: "Distinct roles of the co-activators p300 and CBP in retinoic acid-induced F9 cells" Nature. 393. 284-289 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Handa,H.,et al.: "DNA binding proteins.In HIgh Resolution Choromatography" Oxford University Press., (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 半田宏.他: "RNA構造とスプライシング細胞機能と代謝マップ" 東京化学同人, (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] T.Kamura et al.: "Characterization of the human thrombopoietin gene promoter." J.Biol.Chem.272. 11361-11368 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] M.Murakami et al.: "Phosphorylation and high level expression of Fra-2 in v-src tronsformed cells : a pathway of activation of endogenous AP-1." Oncogene. 14. 2435-2444 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] F.Q.Li et al.: "Molecular cloning of MBF2 reveals its contact with TFIIA that leads to transcriptionel activation." Genes to Cells. 2. 143-153 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Y.Yamaguchi et al.: "Autoregulation of Pax6 transcripticnal activation by two distinct DNA-binding subdomains of the paired donain." Cenes to Cells. 2. 255-261 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Y.Sowa et al.: "Retinoblaston a binding factor 1 site in the core promoter region of the Ruman RB gave is activated by hGABP/E4TF1." Concer Res.57. 3145-3148 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] M.Hiramoto et al.: "Nuclear targeted suppression of NF-EB activity by the novel quinone derivative E3330." J.Immunol.160. 810-819 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] 半田 宏: "わかりやすい遺伝子工学" 昭晃堂, 212 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 和田忠士, 半田 宏, 他: "転早因子研究の最前線" 羊土社, 220 (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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