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Analysis of viral counter regulation against host's defense mechanism for viral infection using HCV transgenic mouse model

Research Project

Project/Area Number 09470088
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Virology
Research InstitutionTokyo Metropolitan Institute for Neuroscience (1998)
Tokyo Metropolitan Organization for Medical Research (1997)

Principal Investigator

WAKITA Takaji  Tokyo Metropolitan Institute for Neuroscience, Department of Microbiology and Immunology, Research Scientist, 微生物学・免疫学研究部門, 研究員 (40280789)

Co-Investigator(Kenkyū-buntansha) TANAKA Nobuyuki  University of Tokyo, Department of Immunology, Associate Professor, 医学部・免疫学教室, 助教授 (80222115)
TAYA Choji  Tokyo Metropolitan Institute of Medical Science, Department of Laboratory Animal, 実験動物研究部門, 研究員 (90175456)
TANAKA Yoshikazu  Tokyo Metropolitan Institute of Medical Science, Department of Microbiology, Res, 放射線医学研究部門, 研究員 (50291159)
KOHARA Michinori  Tokyo Metropolitan Institute of Medical Science, Department of Microbiology, Hea, 放射線医学研究部門, 研究員 (10250218)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥10,400,000 (Direct Cost: ¥10,400,000)
Fiscal Year 1998: ¥4,800,000 (Direct Cost: ¥4,800,000)
Fiscal Year 1997: ¥5,600,000 (Direct Cost: ¥5,600,000)
KeywordsHCV / TRANSGENIC MOUSE / Cre / loxP / CTL
Research Abstract

Missing of proper culture system and animal model has hampered fine analysis of viral life cycle and pathogenesis of HCV infection. We established HCV transgenic mouse model using Cre/loxP switching system. We put neomycin resistance gene as a stuffer flanked by loxP sequence at both 5' and 3' end between CAG promoter and core to NS2, HCV cDNA CN2. Spleens were harvested from CN2 transgenic mice. In 2 lineages out of 8, cultured spleen cell and fibroblast highly expressed core, E1 and E2 proteins by western blot and immunostaining after infection with recombinant adeno virus (AxCANCre) which express cre DNA recombinase in infected cell. To induce HCV expression in vivo and in the liver, we injected AxCANCre to CN2 transgenic mouse from tail vein and HCV protein expression was confirmed in its liver tissue. With the expression of HCV structural protein, acute liver injury which was similar to human acute viral hepatitis was occurred. It was accompanied with apoptotic liver cell death. Serum core protein was detected in transgenic mice 7 days after AxCANCre injection with evident serum ALT elevations, subsequently, anti-core antibody response was detected at 14 days after infection. Furthermore, CD4 and CD8 positive cell depletion assay normalized the serum ALT elevations of mice and pathological changes in the liver. These results suggest that HCV proteins are not direct cytopathic and the host immune response has a pivotal roles in HCV infection. This transgenic mouse model system allows us to analyze the functions of viral products and host immune system in HCV infection and hepatocellular carcinogenesis of HCV.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (95 results)

All Other

All Publications (95 results)

  • [Publications] Wakita T: "Antiviral effects of antisense RNA on hepatitis C virus RNA translation and expression." Journal of Medical Virology. 57. 217-222 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Wakita T: "Efficient conditional transgene expression in hepatitis C virus transgenic mice mediated by the Cre/loxP system." Journal of Biological Chemistry. 273. 9001-9006 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yasui K: "The native form and maturation process of hepatitis C virus core protein" Journal of Virology. 72. 6048-6055 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Moradpour D: "Tightly regulated expression of the entire hepatitis C virus structural region." Biochem.Biophys.Res.Commun.246. 920-924 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Geissler M: "Differential cellular and humoral immune responses to HCV core and HBV envelope proteins after genetic immunizations using chimeric constructs." Vaccine. 16. 857-867 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Young L.OH: "Determination of functional domains in polypyrimidin-tract-binding protein." Biochem.J.331. 169-175 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nozawa H: "Functionally inactivating point mutation in the tumor-supressor IRF-1 gene identitied in human gastric cancer." Int.J.Cancer. 77. 522-527 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Park KC: "Regulation of interferon responses in medulloblastoma cells by interferon regulatory factor-1 and -2." Br.J.Cancer. 77. 2081-2087 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Sato M: "Involvement of the IRF family transcription factor IRF-3 in virus-induced activation of the IFN-beta gene." FEBS Lett.425. 112-116 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Sato M: "Positive feedback regulation of type I IFN genes by the IFN-inducible transcription factor IRF-7." FEBS Lett.441. 106-110 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Liu-ZJ: "A critical role for cyclin C in promotion of the hematopoietic cell cycle by cooperation with c-Myc." Mol-Cell-Biol.18. 3445-3454 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ueda-T: "Production of immunoreactive 2',5'-oligoadenylate synthetase in p48-deficient mice." J-Interferon-Cytokine-Res.18. 181-185 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kondo Y: "Inhibition of telomerase increases the susceptibility of human malignant glioma cells to cisplatin-induced apoptosis." Oncogene. 16. 2243-2248 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ishii,S: "α-Galactosidase transgenic mouse: Heterogeneous gene expression and post-translational gycosylation in tissues." Glycoconjugate J.15. 591-594 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kase,R: "Immunohistochemical characterization of transgenic mice highly expressing human lysosomal α-galactosidase." Biochim.Biophys.Acta. 1406. 260-266 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kuida,K: "Reduced apoptosis and cytochrome c-mediated caspase activation in mice lacking caspase 9." Cell. 94. 325-337 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Mochizuki,T: "Cloning of inv,a gene that controls left/right asymmetry and kidney development." Nature. 395. 177-181 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tanaka N: "Typelinterferons are essential mediators of apoptotic death in virally infected cells." Genes to Cells. 3. 29-37 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tanaka T: "Interferon Treatment of Patients Co-infected with HGV and HCV: Correlation of Treatment Response with Serum Anti-HGV Envelope(E2)Protein Antibody and Genomic." J.Med.Virol.(in press). (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Takeuchi T: "Real-Time Detection System for Quantification of Hepatitis C Virus Genome." Gastroenterology. (in press). (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Inoue K: "Etiological relationship between GB virus C and fulminant hepatitis." J.Hepatology. (in press). (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kondo S.: "Antisense telomerase treatment ; induction of two distinct pathways, apoptosis and differentiation" FASEB J. 12. 801-811 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kondo S: "FADD gene therapy for malignant gliomas in vitro and in vivo." Human Gene Therapy. 9. 1599-1608 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kondo S: "Retrovial Transfer of Cpp32 β Gene into Malignant Gliomas in vitro and in vivo." Cancer Research. 58. 962-967 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Taniguti T: "Regulation of the interferon system,immune response and oncogenesis by the transcription factor interferon regulatory factor-1." Eur.Cytokine.Netw.9. 43-48 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Harada H: "The role of interferon regulatory factors in the interferon system and cell growth control." Biochimie. 80. 641-650 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 脇田隆字: "Cre/loxシステムを利用したHCVトランスジェニックマウスの確立" ウイルス. 48. 9-18 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tokushige K et al: "Comparison between cytomegalovirus promoter and elongation factor-1 alpha promoter-driven constructs in the establishment of cell lines expressing hepatitis C virus core protein." Journal of Virological Methods. 64. 73-80 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Akiyama, H.et al: "Effects of cyclosporin A on hepatitis C virus infection in bone marrow transplantation patients." Bone Marrow Transplant.20. 993-995 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Moradpour D et al: "Tightly regulated expression of the entire hepatitis C virus structural region." Biochem.Biophys.Res.Commun. 246. 920-924 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Wakita T et al: "Efficient conditional transgene expression in hepatitis C virus transgenic mice mediated by the Cre/loxP system" J Biol Chem. 273. 9001-9006 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Geissler M et al: "Differential cellular and humoral immune responses to HCV core and HBV envelope proteins after genetic immunizations using chimeric constructs." Vaccine. 16. 857-867 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yasui K et al: "The native form and maturation process of hepatitis C virus core protein J." Virol.72. 6048-6055 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Wakita T et al: "Antiviral effects of antisense RNA on hepatitis C virus RNA translation and expression." J.Med.Virol.57. 217-222 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] G.Barba et al: "Hepatitis C Virus Core Protein shows a Cytoplasmic Localization and Associates to Cellular Lipid Storage Droplets." Proc.Natl.Acad.Sci.USA.94. 1200-1205 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] N Kamoshita et al: "Genetic analysis of internal ribosomal entry site on hepatitis C virus RNA : implication for involvement of the highly-ordered structure and cell type specific trans-acting factors." Virology. 233. 9-18 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] T Tanaka et al: "Acute hepatitis caused by sexual or household transmission of GBV-C J." Hepatology. 27. 1110-1112 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] M.Yamauchi et al: "Serum hepatitis C virus core protein level during Interferon-a therapy in patients with chronic hepatitis C : relationship between biochemical and virologic responses." Hepatology Res.8. 189-197 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Young L.OH et al: "Determination of functional domains in polypyrimidin-tract-binding protein." Biochem.J.331. 169-175 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] T Tanaka et al: "Interferon Treatment of Patients Co-infected with HGV and HCV : Correlation of Treatment Response with Serum Anti-HGV Envelope (E2) Protein Antibody and Genomic RNA." J.Med.Virol.(in press.). (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] T Takeuchi et al: "Real-Time Detection System for Quantification of Hepatitis C Virus Genome." Gastroenterology. (in press). (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] K Inoue et al: "Etiological relationship between GB virus C and fulminant hepatitis.J." Hepatology. (in press). (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kondo S.et al: "Antisense telomerase treatment ; induction of two distinct pathways, apoptosis and differentiation." FASEB J.12. 801-811 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kondo S.et al: "FADD gene therapy for malignant gliomas in vitro and in vivo." Human Gene Therapy. 9. 1599-1608 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kondo S.et al: "Retrovial Transfer of Cpp32beta Gene into Malignant Gliomas in vitro and in vivo." Cancer Research.58. 962-967 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kondo Y.et al: "Inhibition of telomerase increases the susceptibility of human malignant glioma cells to cisplatin-induced apoptosis." Oncogene. 16. 2243-248 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Shimmoto, M.et al: "Generation and characterization of transgenic mice expressing a human mutant alpha-galactosidase with an R301Q substitution causing a variant form of Fabry disease." FEBS Lett.417. 89-91 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ishii, S.et al: "alpha-Galactosidase transgenic mouse : Heterogeneous gene expression and posttranslational glycosylation in tissues." Glycoconjugate J.15. 591-594 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kase, R et al: "Immunohistochemical characterization of transgenic mice highly expressing human lysosomal alpha-galactosidase" Biochim.Biophys.Acta. 1406. 260-266 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kuida, K et al: "Reduced apoptosis and cytochrome c-mediated caspase activation in mice lacking caspase 9" Cell. 94. 325-337 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Mochizuki, T.et al: "Cloning of inv, a gene that controls left/right asymmetry and kidney development" Nature. 395. 177-181 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tanaka N et al: "Type I interferons are essential mediators of apoptotic death in virally infected cells" Genes-Cells. 3. 29-37 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nozawa H et al: "Functionally inactivating point mutation in the tumor-suppressor IRF-1 gene identified in human gastric cancer" Int-J-Cancer. 77. 522-7 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Park KC et al: "Regulation of interferon responses in medulloblastoma cells by interferon regulatory factor-1 and -2" Br J Cancer. 77. 2081-2087 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Sato-M et al: "Positive feedback regulation of type I IFN genes by the IFN-inducible transcription factor IRF-7" FEBS-Lett. 441. 106-10 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Sato-M et al: "Involvement of the IRF family transcription factor IRF-3 in virus-induced activation of the IFN-beta gene." FEBS-Lett.425. 112-6 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Liu-ZJ et al: "A critical role for cyclin C in promotion of the hematopoietic cell cycle by cooperation with c-Myc." Mol-Cell-Biol.18. 3445-54 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ueda-T et al: "Production of immunoreactive 2', 5'-oligoadenylate synthetase in p48-deficient mice." J-Interferon-Cytokine-Res.18. 181-5 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Taniguchi-T et al: "Regulation of the interferon system, immune response and oncogenesis by the transcription factor interferon regulatory factor-1" Eur.Cytokine Netw.9. 43-48 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Harada-H et al: "The role of interferon regulatory factors in the interferon system and cell growth control" Biochimie.80. 641-650 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Wakita T: "Antiviral effects of antisense RNA on hepatitis C virus RNA translation and expression." Journal of Medical Virology. 57. 217-222 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Wakita T: "Efficient conditional transgene expression in hepatitis C virus transgenic mice mediated by the Cre/loxP system." Journal of Biological Chemistry. 273. 9001-9006 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Yasui K: "The native form and maturation process of hepatitis C virus core protein" Journal of Virology. 72. 6048-6055 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Moradpour D: "Tightly regulated expression of the entire hepatitis C virus structural region." Biochem.Biophys.Res.Commun.246. 920-924 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Geissler M: "Differential cellular and humoral immune responses to HCV core and HBV envelope proteins after genetic immunizations using chimeric constructs." Vaccine. 16. 857-867 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Young L.OH: "Determination of functional domains in polypyrimidin-tract-binding protein." Biochem.J.331. 169-175 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Tanaka T: "Interferon Treatment of Patients Co-infected with HGV and HCV: Correlation of Treatment Response with Serum Anti-HGV Envelope(E2)Protein Antibody and Genomic RNA." J.Med.Virol.(in press). (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Takeuchi T: "Real-Time Detection System for Quantification of Hepatitis C Virus Genome." Gastroenterology. (in press). (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Inoue K: "Etiological relationship between GB virus C and fulminant hepatitis." J.Hepatology. (in press). (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kondo S.: "Antisense telomerase treatment; induction of two distinct pathways, apoptosis and differentiation" FASEB J. 12. 801-811 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kondo S: "FADD gene therapy for malignant gliomas in vitro and in vivo." Human Gene Therapy. 9. 1599-1608 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kondo S: "Retrovial Transfer of Cpp32 β Gene into Malignant Gliomas in vitro and in vivo." Cancer Research. 58. 962-967 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kondo Y: "Inhibition of telomerase increases the susceptibility of human malignant glioma cells to cisplatin-induced apoptosis." Oncogene. 16. 2243-2248 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Ishii,S: "α-Galactosidase transgenic mouse: Heterogeneous gene expression and post-translational glycosylation in tissues." Glycoconjugate J.15. 591-594 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kase,R: "Immunohistochemical characterization of transgenic mice highty expressing human lysosomal α-galactosidase." Biochim.Biophys.Acta. 1406. 260-266 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kuida,K: "Reduced apoptosis and cytochrome c-mediated caspase activation in mice lacking caspase 9." Cell. 94. 325-337 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Mochizuki,T: "Cloning of inv, a gene that controls left/right asymmetry and kidney development." Nature. 395. 177-181 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Tanaka N: "Typelinterferons are essential mediators of apoptotic death in virally infected cells." Genes to Cells. 3. 29-37 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Nozawa H: "Functionally inactivating point mutation in the tumor-supressor IRF-1 gene identified human gastric cancer." Int.J.Cancer. 77. 522-527 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Park KC: "Regulation of interferon responses in medulloblastoma cells by interferon regulatory factor-1 and -2." Br.J.Cencer. 77. 2081-1087 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Sato M: "Involvement of the IRF family transcription factor IRF-3 in virus- induced activation of the IFN-beta gene." FEBS Lett.425. 112-116 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Sato M: "Positive feedback regulation of type I IFN genes by the IFN-inducible transcription factor IRF-7." FEBS Lett.441. 106-110 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Liu-Zj: "A critical role for cyclin C in promotion of the hematopoietic cell cycle by cooperation with c-Myc." Mol-Cell-Biol.18. 3445-3454 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Ueda-T: "Production of immunoreactive 2',5'-oligoadenylate synthetase in p48-deficient mice." J-Interferon-Cytokine-Res. 18. 181-185 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Taniguti T: "Regulation of the interferon system, immune response and oncogenesis by the transcripton factor interferon regulatory fector-1." Eur.Cytokine.Netw.9. 43-48 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Harada H: "The role of interferon regulatory factors in the interferon system and cell growth control." Biochimie. 80. 641-650 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 脇田隆字: "Cre/loxPシステムを利用したHCVトランスジェニックマウスの確立" ウイルス. 48. 9-18 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Wakita,T.: "Efficient conditional transgene expression in hepatitis C virus transgenic mice mediated by the Cre/loxP system." Journal of Biological Chemistry. (in press). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Wakita,T.: "Antiviral effects of antisense RNA on hepatitis C virus RNA translation and expression." Journal of Medical Virology. (in press). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Tokushige,K: "Comparison between cytomegalovirus promoter and elongation factor-1 alpha promoter-driven constructs in the establishment of cell lines expressing hepatitis C virus core protein" Journal of Virological Methods. 64. 73-80 (1997)

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      1997 Annual Research Report
  • [Publications] Akiyama,H.: "Effects of cyclosporin A on hepatitis C virus infection in bone marrow transplantation patients." Bone Marrow Transplant. 20. 993-995 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Barba,G.: "Hepatitis C Virus Core Protein shows a Cytoplasmic Localization and Associates to Celular Lipid Storage Droplets." Proc.Natl.Acad.Sci.USA.94. 1200-1205 (1997)

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      1997 Annual Research Report
  • [Publications] Kamoshita,N.: "Genetic analysis of intemal ribosomal entry site on hepatitis C virus RNA:implication for involvement of the highly-ordered structure and cell type specific trans-acting factors." Virology. 233. 9-18 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 小原,道法: "Modern Physician" C型肝炎とG型肝炎検査法の進歩, 5 (1997)

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      1997 Annual Research Report
  • [Publications] 小原,道法: "南江堂" C型肝癌発生とウイルス増殖, 4 (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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