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Forensic Studies on Identification of a Suspect in a Sexual Crime by Typing of DNA Polymorphisms and Biochemical Genetic Markers

Research Project

Project/Area Number 09470122
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Legal medicine
Research InstitutionGunma University

Principal Investigator

YASUDA Toshihiro  Gunma University School of Medicine, Department of Legal Medicine, Associate Professor, 医学部, 助教授 (80175645)

Co-Investigator(Kenkyū-buntansha) NAKAJIMA Tamiko  Gunma University School of Medicine, Department of Legal Medicine, Assistant, 医学部, 助手 (40008561)
TAKESHITA Haruo  Gunma University School of Medicine, Department of Legal Medicine, Lecturer, 医学部, 講師 (90292599)
KISHI Koichiro  Gunma University School of Medicine, Department of Legal Medicine, Professor, 医学部, 教授 (30169841)
細見 修  群馬大学, 医学部, 助手 (30134274)
Project Period (FY) 1997 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥10,000,000 (Direct Cost: ¥10,000,000)
Fiscal Year 1999: ¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1998: ¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 1997: ¥4,200,000 (Direct Cost: ¥4,200,000)
KeywordsPersona identification / Sexual crime / Deoxyribonuclease / Semen / Genetic polymorphism / Molecular biology / Human genetics / Genetic markers / 血液型 / DNA型 / 遺伝子 / cDNA構造
Research Abstract

1. We have previously found human deoxyribonuclease (DNase) II to be a genetic marker present in semen. In this study, we succeeded in cloning of both the complementary and genomic DNAs encoding human DNase II. Furthermore, regional localization of the gene (DNASE2) to 19p13.2-p13.1 was achieved by FISH analysis. This is the first report of the cloning and characterization of the cDNA and gene for mammalian DNase II.
2. DNase II levels in human vary depending on whether the individual has the DNASE2ィイD1*ィエD1H or ィイD1*ィエD1L allele. The transient transfection luciferase analysis of the DNase II gene expression permit us to conclude that the A-75G transition in the proximal promoter region causes the allelic difference in the promoter activity of the gene, underlying its genetic polymorphism.
3. Human DNase II was composed of three non-identical subunits. From site-directed mutagenesis study, it was found that a simultaneous attachment of a carbohydrate moiety, proteolytic cleavage and the … More inherent signal peptide might be required for subcellular sorting and maturation of the enzyme.
4. Since two new alleles, DNASEIィイD1*ィエD15 and ィイD1*ィエD16, of human DNase I polymorphism were identified, DNase I-polymorphism can be classified into 21 different phenotypes. These findings permit the ability of DNase I-polymorphism in personal identification to be further elevated. The type 6 isoenzyme was the first type to be shown to be labile.
5. The findings that Caucasian and Negroid populations were classified into several DNase I phenotypes found DNase -polymorphism to be world-wide. Furthermore, we found a geographical north-south decline in DNase I 2 allele frequency in Japanese populations.
6. Two short tandem repeat systems, HumTPO and HumLPL, were investigated in Japanese populations by means of newly developed genotyping method. Duplex typing of these systems were found to be very powerful in forensic utility.
Considering these findings made in this research project, both DNases I and II have been confirmed to be one of the most useful and effective genetic markers suited for personal identification from forensic semen samples. Less

Report

(4 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • 1997 Annual Research Report
  • Research Products

    (139 results)

All Other

All Publications (139 results)

  • [Publications] Toshihiro Yasuda: "Purification of deoxyribonuclease I(DNase I) using two-step chromatography"Analytical Biochemistry. 245(2). 255-257 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Aoyama Masahiro: "DYS19 marker in Japanese populations"International Journal of Legal Medicine. 110(2). 112-113 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Toshihiro Yasuda: "Population studies of human deoxyribonuclease I polymorphism"Human Heredity. 47(3). 121-124 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Haruo Takeshita: "Mouse deoxyribonuclease I(DNase I)"Biochemistry and Molecular Biology International. 42(1). 65-75 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Toshihiro Yasuda: "Rabbit deoxyribonuclease I(DNase I)"Biochemical Journal. 325(2). 465-473 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Reiko Iida: "The fifth allele of the human deoxyribonuclease I(DNase I) polymorphism"Electrophoresis. 18(11). 1936-1939 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Souichi Tsutsumi: "Phenotype 2 of deoxyribonuclease I may be used as a risk factor of gastric carcinoma"Cancer. 82(9). 1621-1625 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Tamiko Nakajima: "Two novel sereening methods for selecting monoclonal antibodies which specifically inhibit DNase I enzyme activity"Immunological Investigation. 27(3). 145-152 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Toshihiro Yasuda: "Structure and organization of the human deoxyribonuclease II (DNase II) gene"Annals of Human Genetics. 63(4). 299-305 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Osamu Shimada: "Detection of deoxyribonuclease I along the secretory pathway in Paneth cells of human small intestine"The Journal of Histochemistry and Cytochmistry. 46(7). 833-840 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Reiko Iida: "Detection of isoenzymes of deoxyribonucleases I and II electrophoresed gels with picogram sensitivity using SYBR Green I"Electrophoresis. 19(14). 299-305 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Haruo Takeshita: "Identification of the three non-identical subunits constituting human deoxyribonuclease II"FEBS Letters. 440(1/2). 239-242 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Haruo Takeshita: "The STR loci HumTPO and HumLPL : population genetic data in eight populations"International Journal of Legal Medicine. 110(6). 331-333 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Osamu Hosomi: "Simple purification method of the anriphospholpied antibody from normal human plasma"Experimental and Clinical Immunogenetics. 14(4). 281-285 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Toshihiro Yasuda: "Activity measurement for deoxyribonucleases I and II with picogram sensitivity based on SYBR Green I fluorescence"Analytical Biochemistry. 255(2). 274-276 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Toshihiro Yasuda: "Molecular cloning of the cDNA encoding human deoxyribonuclease II"The Journal of Biological Chemistry. 273(5). 2610-2616 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Toshihiro Yasuda: "Chromosomal localization of a human deoxyribonuclease II gene (DNASE2) to 19p13.2-p13.1 using both the polymerase chaine reaction and fluorescence in situ hybridization analysis"Biochemical and Biophysical Research Communications. 244(3). 815-818 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Haruo Takeshita: "Detection of the two short tandem repeat loci (HumTOP and HumLPL) in Japanese populations using discontinuous polyacrylamid gel electrophoresis"The Japanese Journal of Legal Medicine. 52(2). 139-143 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Reiko Iida: "Deoxyribonuclease I phenotyping from saliva stains"The Journal of Forensic Sciences. 44(1). 175-178 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Toshihiro Yasuda: "Structural requirement of a human deoxyribonuclease II for the development of the active enzyme form, revealed by site-directed mutagenesis"Biochemical and Biophysical Research Communications. 256(3). 591-594 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Osamu Hosomi: "Xenopus lavis pancreatic DNase I"Experimental and Clinical Immunogenetics. 16(2). 117-123 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Toshihiro Yasuda: "A new allele, DNASE1^*6, of human deoxyribonuclease I polymorphism encodes an Arg to Cys substitution responsible for its instability"Biochemical and Biophysical Research Communications. 260(1). 280-283 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Haruo Takeshita: "Simultaneous typing of the STR loci, vWF and HumTPO, using discontinuous polyacrylamide gel electrophoresis"Legal Medicine. 1(3). 233-237 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yoshimitsu Nakashima: "Molecular, biochemical and immunological studies of hen pancreatic deoxyribonuclease I"International Journal of Biochemistry and Cell Biology. 31(11). 1313-1324 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Tamiko Nakajima: "Rapid purification of human DNase I using mouse monoclonal anti-DNase I antibodies and characterization of the antibodies"Experimental and Clinical Immunogenetics. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Reiko Iida: "Age-related changes of gene expression in mouse kidney : fluorescence differential display-PCR analysis"Mechanisms of Ageing and Development. 113(2). 133-142 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Toshihiro Yasuda: "Activity staining for detection of ribonucleases using the dried agarose film overlay method after electrophoresis"Methods in Enzymology. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Toshihiro Yasuda: "The molecular basis for genetic polymorphism of human deoxyribonuclease II (DNase II)"FEBS Letters. 467(1/2). 231-234 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Toshihiro Yasuda: "Molecular cloning of cDNA encoding Xenopus laevis deoxyribonuclease I"DNA Sequence. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Haruo Takeshita: "Geographical north-south decline in DNase I allele 2 in Japanese populations"Human Biology. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 岸 紘一郎: "核酸分解酵素DNaseIの遺伝的多型の発見"医学のあゆみ. 180(10). 658-659 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 安田 年博: "ヒトdeoxyribonuclease II(DNase II)に関する最近の研究"北関東医学. 49(1). 39-41 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 岸 紘一郎: "DNaseI"日本臨床. 57(増刊). 486-489 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 飯田 礼子: "PCR-SSCP法によってヒトdeoxyribonuclease I遺伝子の第7イントロン内に検出される新しい多型部位"DNA多型. Vol.5. 203-206 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 飯田 礼子: "PCR-SSCP法によって検出されたdeoxyribonuclease I遺伝子の新規なミスセンス差異"DNA多型. Vol.6. 132-135 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 竹下 治男: "不連続系ポリアクリルアミドゲル電気泳動を利用した2種類のSTR座位(vWFおよびHumTPO)の同時検出"DNA多型. Vol.7. 88-90 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Haruo Takeshita: "Postmortem absorption of dichloromethane : a case study and animal experiment"International Journal of Legal Medicine. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Toshihiro Yasuda: "Genetic polymorphisms detected in human urine : their appolication to forensic individualization"The Japanese Journal of Legal Medicine. 51(6). 407-416 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Toshihiro Yasuda: "Anthropological applications of genetic polymorphisms detected in human urine : a review of Japanese studies"Revista di Antropologia. 75(1). 407-416 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 灘野 大太: "アポトーシスとエンドヌクレアーゼとの関連性"臨床薬理の進歩. No.18. 105-110 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 安田 年博: "ヒト脳下垂体には高活性なdeoxyribonuclease Iが存在する"日本法医学雑誌. 52(2). 295-300 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 藤川 克行: "日本人のトランスフェリン多型に認められる地理的勾配(埼玉県および青森県在住者を中心として)"血液事業. 21(4). 279-287 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 竹下 治男: "DNASE1^*6遺伝子がつくる酵素の寿命は短い(印刷中)"DNA多型. Vol.8.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 飯田 礼子: "年齢依存性を示すマウス腎の新規な遺伝子検索(印刷中)"DNA多型. Vol.8.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Haruo Takeshita: "Mammlian deoxyribonucleases I are classified into three types : pancreas, parotid and pancreas-parotid(mixed) types, based on differences of their tissue concentrations (in press)"Biochemical and Biophysical Research Communications.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] T. Yasuda, H. Takeshita, T. Nakajima, O. Hosomi, Y. Nakashima, K. Kishi.: "Purification of deoxyribonuclease I (DNase I) using two-step chromatography"Anal. Biochem.. 245(2). 121-124 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] M. Aoyama, T. Matsuki, K. Kishi, R. Iida, E. Tsubota.: "DYS19 marker in Japanese populations"Int. J. Legal Med.. 110(2). 112-113 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] T. Yasuda, H. Takeshita, R. Iida, I. Yuasa, K. Kishi.: "Population studies of human deoxyribonuclease I polymorphism"Hum. Hered.. 47(3). 121-124 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] H. Takeshita, T. Yasuda, T. Nakajima, O. Hosomi, Y. Nakashima, K. Kishi.: "Mouse deoxyribonuclease I (DNase I) : biochemical and immunological characterizations, cDNA structure and tissue distribution"Biochem. Mol. Biol. Int.. 42(1). 65-75 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] T. Yasuda, H. Takeshita, T. Nakajima, O. Hosomi, Y. Nakashima, K. Kishi.: "Rabbit deoxyribonuclease I (DNase I) : purification from urine, immunological and proteochemical characterization, nucleotide sequence, expression in tissues, relationships with other DNases I and phylogenetic analysis"Biochem. J.. 325(2). 465-473 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] R. Iida, T. Yasuda, M. Aoyama, E. Tsubota, M. Kobayashi, I. Yuasa, K. Kishi.: "The fifth allele of the human deoxyribonuclease I (DNase I) polymorphism"Electrophoresis. 18(11). 1936-1939 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] H. Takeshita, E. Meyer, B. Brinkmann.: "The STR loci HumTPO and HumLPL : population genetic data in eight populations"Int. J. Legal Med.. 110(6). 331-333 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] O. Hosomi, A. Takeya, T. Yasuda, S. Yazawa, T. Nakajima, T. Kogure, H. Iwai, K. Kishi.: "Simple purification method of the antiphospholipid antibody from normal human serum"Exp. Clin. Immunogenet.. 14(4). 281-285 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] T. Yasuda, H. Takeshita, E. Nakazato, T. Nakajima, O. Hosomi, Y. Nakashima, K. Kishi.: "Activity measurement for deoxyribonucleases I and II with picogram sensitivity based on DNA/SYBR Green I fluorescence"Anal. Biochem.. 255(2). 274-276 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] T. Yasuda, H. Takeshita, T. Nakajima, O. Hosomi, Y. Nakashima, K. Kishi.: "Molecular cloning of the cDNA encoding human deoxyribonuclease II"J. Biol. Chem.. 273(5). 2610-1616 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] T. Yasuda, H. Takeshita, R. Iida, T. Nakajima, O. Hosomi, Y. Nakashima, K. Mogi, K. Kishi.: "Chromosomal localization of a human deoxyribonuclease II gene (DNASE2) to 19p13.2-p13.1 using both the polymerase chain reaction and fluorescence in situ hybridization analysis"Biochem. Biophys. Res. Commun.. 244(3). 815-818 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] H. Takeshita, T. Yasuda, T. Nakajima, O. Hosomi, Y. Nakashima, S. Tsutsumi, K. Kishi.: "Detection of the two short tandem repeat loci (HumTPO and HumLPL) in Japanese populations using discontinuous polyacrylamide gel electrophoresis"Jpn. J. Legal Med.. 52(2). 139-143 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] S. Tsutsumi, T. Asao, Y. Nagamachi, T. Nakajima, T. Yasuda, K. Kishi.: "Phenotype 2 of deoxyribonuclease I may be used as a risk factor of gastric carcinoma"Cancer. 82(9). 1621-1625 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] T. Nakajima, T. Yasuda, Y. Nakashima, O. Hosomi, H. Takeshita, K. Kishi.: "Two novel screening methods for selecting monoclonal antibodies which specifically inhibit DNase I enzyme activity"Immunol. Invest.. 27(3). 145-152 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] T. Yasuda, H. Takeshita, R. Iida, S. Tsutsumi, T. Nakajima, O. Hosomi, S. Mori, K. Kishi.: "Structure and organization of the human deoxyribonuclease II (DNase II) gene"Ann. Hum. Genet.. 62(4). 299-305 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] O. Shimada, H. Ishikawa, H. Shimada, T. Yasuda, K. Kishi, S. Suzuki.: "Detection of deoxyribonuclease I along the secretory pathway in Paneth cells of human small intestine"J. Histochem. Cytochem. 46(7). 833-840 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] R. Iida, T. Yasuda, E. Tsubota, Y. Nakashima, K. Sawazaki, M. Aoyama, T. Matsuki, K. Kishi: "Detection of isoenzymes of deoxyribonucleases I and II on electrophoresed gels with picogram sensitivity using SYBR Green I"Electrophoresis. 19(14). 299-305 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] H. Takeshita, T. Yasuda, R. Iida, T. Nakajima, O. Hosomi, Y. Nakashima, S. Mori, K. Kishi.: "Identification of the three non-identical subunits constituting human deoxyribonuclease II"FEBS Lett.. 440(1/2). 239-242 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] R. Iida, K. Sawazaki, T. Yasuda, E. Tsubota, M. Aoyama, T. Matsuki, K. Kishi.: "Deoxyribonuclease I phenotyping from saliva stains"J. Forensic Sci.. 44(1). 175-178 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] T. Yasuda, H. Takeshita, R. Iida, T. Nakajima, O. Hosomi, Y. Nakashima, S. Mori, K. Kishi.: "Structural requirement of a human deoxyribonuclease II for the development of the active enzyme form, revealed by site-directed mutagenesis"Biochem. Biopjys. Res. Commun.. 256(3). 591-594 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] O. Hosomi, T. Yasuda, H. Takeshita, T. Nakajima, Y. Nakashima, Y. Hanaoka, K. Kishi.: "Xenopus laevis pancreatic DNase I : purification and characterization"Exp. Clin. Immunogenet.. 16(2). 117-123 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] T. Yasuda, H. Takeshita, R. Iida, S. Kogure, K. Kishi.: "A new allele, DNASEィイD1*ィエD16, of human deoxyribonuclease I polymorphism encodes an Arg to Cys substitution responsible for its instability"Biochem. Biophys. Res. Commun.. 260(1). 280-283 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] H. Takeshita, T. Yasuda, T. Nakajima, Y. Nakashima, S. Mori, K. Mogi, K. Kishi.: "Simultaneous typing of the STR loci, vWF and HumTPO, using discontinuous polyacrylamide gel electrophoresis"Legal Med.. 1(3). 233-237 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Y. Nakashima, T. Yasuda, H. Takeshita, T. Nakajima, O. Hosomi, S. Mori, K. Kishi.: "Molecular, biochemical and immunological studies of hen pancreatic deoxyribonuclease I"Int. J. Biochem. Cell Biol.. 31(11). 1313-1324 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] R. Iida, T. Yasuda, E. Tsubota, T. Matsuki, K. Kishi.: "Age-related changes of gene expression in mouse kidney : fluorescence differential display-PCR analysis"Mech. Aging Develop.. 113(2). 135-144 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] T. Yasuda, H. Takeshita, E. Nakazato, T. Nakajima, Y. Nakashima, S. Mori, K. Mogi, K. Kishi.: "The molecular basis for genetic polymorphism of human deoxyribonuclease II (DNase II)"FEBS Lett.. 467(1/2). 231-234 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] T. Nakajima, T. Yasuda, H. Takeshita, Y. Nakashima, S. Mori, K. Mogi, K. Kishi.: "Rapid purification of human DNase I using mouse monoclonal anti-DNase I antibodies and characterization of the antibodies"Exp. Clin. Immunogenet.. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] T. Yasuda, O. Hosomi, H. Takeshita, T. Nakajima, Y. Nakashima, S. Mori, K. Mogi, K. Kishi.: "Molecular cloning of cDNA encoding Xenopus laevis deoxyribonuclease I"DNA Sequence. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Y. Nakashima, T. Yasuda, H. Takeshita, K. Mogi, K. Kishi, H. Shiono, K. Sagisaka, I. Yuasa, H. Nishimukai, H. Kimura.: "Geographical north-south decline in DNase I allele 2 in Japanese populations"Hum. Biol.. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] H. Takeshita, K. Mogi, T. Yasuda, S. Mori, Y. Nakashima, T. Nakajima, H. Akuzawa, S. Nakajo, Y. Hirota, K. Kishi.: "Postmortem absorption of dichloromethane : a case study and animal experiments"Int. J. Legal Med.. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] H. Takeshita, K. Mogi, T. Yasuda, T. Nakajima, S. Mori, T. Hoshino, K. Kishi.: "Mammalian deoxyribonuclease I are classified into three types."Biochem. Biophys. Res. Commun. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] T. Yasuda, H. Takeshita, K. Kishi.: "Genetic polymorphisms detected in human urine : their application to forensic individualization"Jpn. J. Legal Med.. 51(6). 407-416 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] T. Yasuda, D. Nadano, H. Takeshita, R. Iida, K. Kishi.: "Anthropological applications of genetic polymorphisms detected in human urine : a review of Japanese studies."Rivista di Antropologia. 75(1). 407-416 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] T. Yasuda: "High activity of deoxyribonuclease I identified in human pituitary gland-structure and function"Jpn. J. Legal Med.. 52(2). 295-300 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] K. Fujikawa, A. Ishijima, T. Hattori, T. Yasuda, K. Kishi.: "Geographical cline of transferrin polymorphism in Japanese (especially in Saitama and Aomori prefectures)"Blood Programme. 21(4). 279-287 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] K.Kishi, T. Yasuda.: "Discovery of genetic polymorphism of deoxyribonuclease I"Igaku no Ayumi. 180(10). 658-659 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] T. Yasuda: "Recent study on human deoxyribonuclease II"Kitakanto Med. J.. 49(1). 39-41 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] K. Kishi, T. Yasuda, H. Takeshita.: "DNase I"Nihon Rinsyo. 57(Special Issue). 486-489 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] R. Iida, M. Aoyama, T. Matsuki, T. Yasuda, K. Kishi.: "Detection of a new polymorphic site in intron 7 of the human deoxyribonuclease I gene by PCR-SSCP analysis"DNA Polymorphisms. Vol.5. 203-206 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] R. Iida, M. Aoyama, T. Matsuki, T. Yasuda, K. Kishi.: "A new missense mutation detected in the human deoxyribonuclease I gene by PCR-SSCP analysis"DNA Polymorphisms. Vol.6. 132-135 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] H. Takeshita, T. Yasuda, T. Nakajima, K. Kishi.: "Simultaneous detection of the STR loci vWF and HumTPO using discontinuous polyacrylamide gel electrophoresis"DNA Polymorphisms. Vol.7. 88-90 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Reiko Iida: "Deoxyribonuclease I phenotyping from saliva stains"The Journal of Forensic Sciences. 44(1). 175-178 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Toshihiro Yasuda: "Structural requirement of human deoxyribonuclease II for the development of the active enzyme form, revealed by site-directed mutagenesis"Biochemical and Biophysical Research Communications. 256(3). 591-594 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Osamu Hosomi: "Xenopus laevis pancreatic Dnase I: purification and characterization"Experimental and Clinical Immunogenetics. 19(2). 177-123 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Toshihiro Yasuda: "A new allele, DNASE1^*6, of human deoxyribonuclease I polymorphism encodes an Arg to Cys substitution responsible for its instability"Biochemical and Biophysical Research Communications. 260(1). 280-283 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Haruo Takeshita: "Simultaneous typing of the STR loci, Vwf and HumTPO, using discontinuous polyaerylamide gel electrophoresis"Legal Medicine. 1(3). 233-237 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Yoshimitsu Nakashima: "Molecular, biochemical and immunological studies of hen pancreatic deoxyribonuclease I"International Journal of Biochemistry and Cell Biology. 31(11). 1313-1324 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Tamiko Nakajima: "Rapid purification of human Dnase I using mouse monoclonal anti-Dnase I antibodies and characterization of the antibodies"Ezperimental and Clinical lmmunogenetics. (in press).

    • Related Report
      1999 Annual Research Report
  • [Publications] Reiko Iida: "Age-related changes of gene expression in mouse kidney: fluorescence differential display-PCR analysis"Mechanisms of Ageing and Development. 113(2). 113-142 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Toshihiro Yasuda: "The molecular basis for genetic polymorphism of human deoxyribonuclease ll(Dnase ll)"FEBS Letters. 467(1/2). 231-234 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Osamu Hosomi: "Molecular cloning of cDNA encoding Xenopus laevis deoxyribonuclease l"DNA Sequence. (in press).

    • Related Report
      1999 Annual Research Report
  • [Publications] 藤川克行: "日本人のトランスフェリン多型に認められる地理的勾配(埼玉県および青森県在住者を中心として)"血液事業. 21(4). 279-287 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 安田年博: "ヒトdeoxyribonuclease ll(Dnase ll)に関する最近の研究"北関東医学. 49(1). 39-41 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 岸紘一郎: "Dnase l"日本臨床. 57(増刊). 486-489 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 竹下治男: "不連続系ポリアクリルアミドゲル電気泳動を利用した2種類のSTR座位(vWFおよびHumTPO)の同時検出"DNA多型. 7. 88-90 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Haruo Takeshita: "Postmortem absorption of dichloromethane: a case study and animal experiments"International Journal of Legal Medicine. (in press)

    • Related Report
      1999 Annual Research Report
  • [Publications] Haruo Takeshita: "Mammalian deoxyribonucleases l are classified into three types: pancreas, parotid and pancreas-parotid(mixed) types, based on differences of their tissue concentrations"Biochemical and Biophysical Reseach Communications. (in press)

    • Related Report
      1999 Annual Research Report
  • [Publications] Toshihiro Yasuda: "Activity measurement of deoxyribonucleases I and II with picogram sensitivity based on DNA/SYBR Green I fluorescence" Anal.Biochem.255(2). 274-276 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Toshihiro Yasuda: "Molecular cloning of the cDNA encoding human deoxyribonuclease II" J.Biol.Chem.273(5). 2610-2616 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Toshihiro Yasuda: "Chromosomal localization of a human deoxyribonuclease II gene(DNase II)to 19p13.2-13.1 using both the polymerase chain reaction and fluorescence in situ hybridization analysis" Biochem.Biophys.Res.Commun.244(3). 815-818 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Haruo Takeshita: "Detection of the two short tandem repeat loci(HumTPO and HumLPL)in Japanese populations using discontinuous polyacrlamide gel electrophoresis" Jpn.J.Legal Med.52(2). 139-143 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Shoichi Tsutsumi: "Phenotype 2 of deoxyribonuclease I may be used as a risk factor for gastric carcinoma" Cancer. 82(9). 1621-1625 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Tamiko Nakajima: "Two novel screening methods for selecting monoclonal antibodies which specifically inhibit DNase I enzyme activity" Immunol.Invest.27(3). 145-152 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Toshihiro Yasuda: "Structure and organization of the human deoxyribonuclease II(DNase II)gene" Ann.Hum.Genet.62(4). 299-305 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Reiko Iida: "Detection of isozymes of deoxyribonucleases I and II on electrophoresed gels with picogram sensitivity using SYBR Green I" Electrophoresis. 19(14). 2416-2418 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Osamu Shimada: "Detection of deoxyribonuclease I along the secretory pathway in Paneth cells of human small intestine" J.Histochem.Cytochem.46(7). 833-840 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Haruo Takeshita: "Identification of the three non-identical subunits constituting human deoxyribonuclease II" FEBS Lett.440(1/2). 239-242 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Osamu Hosomi: "Xenopus laevis pancreatic DNase I: purification and characterization" Exp.Clin.Immunogenet.(in press). (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Reiko Iida: "Deoxyribonuclease I phenotyping from saliva stains" J.Forensic Sci.44(1). 175-178 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Toshihiro Yasuda: "High activity of deoxyribonuclease I identified in human pituitary gland-structure and function-" Jpn.J.Legal Med.52(5). 295-300 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 安田年博: "ヒトdeoxyribonuclease II(DNase II)に関する最近の研究" 北関東医学. 49(1). 39-41 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] 飯田礼子: "PCR-SSCP法によって検出されたヒトdeoxyribonuclease I遺伝子内の新規なミスセンス変異" DNA多型. 6. 132-135 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Toshihiro Yasuda: "Structural requirements of a human deoxyribonuclease II for the development of the active enzyme form,revealed by site-directed mutagenesis" Biochem.Biophys Res.Commun.(in press). (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Yasuda,T.: "Purification of deoxyribonuclease I(DNase I)using two-step chromatography" Analytical Biochemistry. 245(2). 255-257 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Aoyama,M.: "DYS19 marker in Japanese populations" International Journal of Legal Medicine. 110(2). 112-113 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yasuda,T.: "Populations studies of human deoxyribonuclease I polymorphism" Human Heredity. 47(3). 121-124 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Takeshita,H.: "Mouse deoxyribonuclease I(DNase I)" Biochemistry and Molecular Biology International. 42(1). 65-75 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Iida,R.: "The fifth allele of the human deoxyribonuclease I(DNase I)polymorphism" Electrophoresis. 18(11). 1936-1939 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yasuda,T.: "Rabbit deoxyribonuclease I(DNase I)" Biochemical Journal. 325(2). 465-473 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Takeshita,H.: "The STR loci HumTPO and HumLPL:population genetic data in eight populations" International Journal of Legal Medicine. 110(6). 331-333 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yasuda,T.: "Activity measurement for deoxyribonucleases I and II with picogram sensitivity based on DNA/SYBR Green I fluorescence" Analytical Biochemistry. 255(2). 274-276 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Hosomi,O.: "Simple purification method of the antiphospholipid antibody from normal human plasma" Experimental and Clinical Immunogenetics. 14(4). 281-285 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yasuda,T.: "Genetic polymorphisms detected in human urine: their application to forensic individualization" The Japanese Journal of Legal Medicine. 51(6). 407-416 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yasuda,T.: "Molecular cloning of the cDNA encoding human deoxyribonuclease II" The Journal of Biological Chemistry. 273(5). 2610-2616 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Tsutsumi,S.: "Phenotype 2 of deoxyribonuclease I may be used as a risk factor of gastric cancer" Cancer. (in press).

    • Related Report
      1997 Annual Research Report
  • [Publications] Takeshita,H.: "Detection of the two short tandem repeat loci(HumTPO and HumLPL)in Japanese populations using discontinuous polyacrylamide gel electrophoresis" The Japanese Journal of Legal Medicine. (in press).

    • Related Report
      1997 Annual Research Report
  • [Publications] Nakajima,T.: "Two novel screening methods for selecting,monoclonal antibodies which specifically inhibit DNase I enzyme activity" Immunological Investigations. (in press).

    • Related Report
      1997 Annual Research Report
  • [Publications] Yasuda,T.: "Chromosomal locallization of a human deoxyribonuclease II gene(DNASE2)to 19p13.2-p13.1 using both the polymerase chain reaction and fluorescence in situ hybridization analysis" Biochemical and Biophysical Research communications. (in press).

    • Related Report
      1997 Annual Research Report
  • [Publications] Yasuda,T.: "Anthropological application of genetic polymorphisms detected in human urine: a review of Japanese studies" Rivista di Antropologia. (in press).

    • Related Report
      1997 Annual Research Report
  • [Publications] 灘野 大太: "アポトーシスとエンドヌクレアーゼとの関連性" 臨床薬理の進歩. No.18. 105-110 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 岸 紘一郎: "核酸分解酵素DNase Iの遺伝的多型の発見" 医学のあゆみ. 180(10). 658-659 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 灘野大太: "ヒトのDNase IおよびIIとアポトーシスとの関連性:ヒトDNase I遺伝子構造とpolymorphic siteの同定" 総合健康推進財団 研究報告書(第11)平成6年度助成事業. 111-119 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 安田年博: "核酸分解酵素の遺伝的多型を利用した膵肝疾患罹患のハイリスクグループ特定に関する予防医学的研究" 膵臓病研究財団第3回研究報告書. No.3. 1-4 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 飯田礼子: "PCR-SSCP法によってヒトdeoxyribonuclease I遺伝子の第7イントロン内に検出される新しい多型部位" DNA多型. Vol.5. 203-206 (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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