| Project/Area Number |
09470127
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
内科学一般
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| Research Institution | Kobe University |
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Principal Investigator |
SHIOZAWA Shunichi Kobe University School of Medicine Professor, 医学部, 教授 (40154166)
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| Co-Investigator(Kenkyū-buntansha) |
GOKO Hideki Kobe University School of Medicine Instructor, 医学部, 助手 (80170447)
TSUKAMOTO Yasuo Kobe University School of Medicine Associate Prof., 医学部, 助教授 (30031149)
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| Project Period (FY) |
1997 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥8,300,000 (Direct Cost: ¥8,300,000)
Fiscal Year 1998: ¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 1997: ¥5,700,000 (Direct Cost: ¥5,700,000)
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| Keywords | rheumatoid arthritis / microsatellite marker / genetic predisposition / disease gene / sib-pair analysis / 慢性関節リュウマチ |
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| Research Abstract |
We have searched the human genome for genes that predispose to rheumatoid arthritis using fluorescence-based microsatellite marker analysis and affected sib-pair linkage study. A panel of 41 Japanese families, each with at least two affected siblings, was typed for genome-wide 358 polymorphic microsatellite marker loci. Markers were amplified by the polymerase chain reaction (PCR) using fluorescence-tagged primers and sized based on the difference of CA repeats on DNA.Linkage analysis was made using maximum lod score (MLS). The MLS for D1S214 and D8S556 was 3.27 and 3.33, while the MLS for HLA-DRBl region was below 3.0. According to detailed analysis by the single point analysis using MAPMAKER/SIBS, the MLS for D1S253 and D1S214 was 3.77 and 3.58. The MLS by the multi-point analysis was 6.13 for D1S253. The-MLS for D8S556 by the single point analysis was 4.20. The MLS for DXS1232 was 2.35 by the single point analysis, whereas the MLS for the region 2cM right to DXS1232 and the region between DXS1227 and DXS1200 was 3.03 and 2.93 by the multi-point analysis. Three principal chromosome regions of linkage, D1S253/214, D8S556 and DXS1232, have been identified which we call RA1, RA2 and RA3 for rheumatoid arthritis disease loci.
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