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Development of histamine H2 receptor-deficient mouse model by gene targeting.

Research Project

Project/Area Number 09470131
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Gastroenterology
Research InstitutionJichi Medical School (1998)
The University of Tokyo (1997)

Principal Investigator

SUGANO Kentaro  Jichi Med.Sch., Faculty Medicine, Professor, 医学部, 教授 (60179116)

Co-Investigator(Kenkyū-buntansha) FUKUSHIMA Yasuhisa  Tokyo Unv., Faculty Medicine, Resident, 医学部, 医員
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥9,600,000 (Direct Cost: ¥9,600,000)
Fiscal Year 1998: ¥3,900,000 (Direct Cost: ¥3,900,000)
Fiscal Year 1997: ¥5,700,000 (Direct Cost: ¥5,700,000)
Keywordshistamine H2 receptor / Gene targeting / 胃酸分泌 / ヒスタミンH2受容体 / 遺伝子
Research Abstract

To analyze the functional role of histamine H2 receptors on the physiological regulations not only in gastrointestinal tract but also in whole body including neurological and hematological systems, we tried to develop a mouse model that lacks histamine H2 receptor. As a first step, we cloned mouse histamine H2 receptor gene including non-translated 5' region. With this gene, we constructed a targeting vector that is introduced in ES cells. After selection of ES cells that showed integration of gene targeting segment, these cells are introduced in blastcysts. Several mice in chimeric state were born, but so far no germline transmission was confirmed. Thus, several other ES clones with integration of targeting vectors have been prepared and injected in blastcysts in order to obtain a mouse with germline transmission of the recombinant gene.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] Fukushima Y, Sugano K et al: "Role of the C terminus in Histamine H2 receptor signaling desensitization and agonist-induced internalization" J.Biol.Chem.272. 19464-19470 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Fukushima Y.Sugano K et al: "Oligometer formation of histamine H2 receptors expressed in Sf9 and COS7 cells." FEBS lett.409. 283-286 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Fukushima Y, Sugano K.et al: "Localization of the histamine H2 receptor, a target for anti-ulcer drugs, in gastric parietal cells." Digestin. (in press). (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Fukushima Y,Asano T,Takata K,Funaki M,Ogihara T,Anai M,TsukudaK,Saitoh T,Katagiri H,Aihara M,Matsuhashi N,Oka Y,Yazaki Y,SuganoK: "Role of the C terminus in Histamine H2 receptor signaling, desensitization, and agonist-induced internalization." J.Biol.Chem.272. 19464-19470 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Fukushima Y,Asano T,Saitoh T,Anai M,Funaki M,Ogihara T,Katagiri H,Matsuhashi N,Yazaki Y,Sgano K: "Oligometer formation of histamine H2 receptors expressed in Sf9 and COS7 cells." FEBS lett.409. 283-286 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Fukushima Y,Ohmachi Y,Asano T,Nawano M,Funaki M,Anai M,Ogihara T,Inukai K,Onishi Y,Sakoda H,Saitoh T,Matsuhashi N,Yazaki Y,Sugano K: "Localization of the histamine H2 receptor, a target for anti-ulcer drugs, in gastric parietal cells." Digestion. (in press). (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] M.Anai Y.Fukushima,et al.: "Different subcellular distribution and reguration of expression insulin receptor substrate 3 from those of IRS-1 and IRS-2." Journal of Biological Chemistry. 273. 29686-29692 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 福嶋康之、他: "ヒスタミンH_2受容体の多量体形成" Therapeutic Research. 19. 197-200 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 福嶋康之、菅野健太郎: "消化性潰瘍-H_2受容体" 現代医療. 30. 320-323 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 福嶋康之、菅野健太郎: "分子生物学的研究に用いられる手法" 医学のあゆみ・消化器疾患ver.2.別冊. 5-9 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Y.Fukushima et al.: "Oligomer formation of histamine H_2 receptors expressed in Sf9 and CO57cells." FEBS Letters. 409. 283-286 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Y.Fukushima et al.: "Role of the C-terminus in histamine H_2 receptor signaling,desensilization,and agorlist induced internilization." J.Biol.Chem.272. 19464-19470 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 福嶋康之 他: "脱感作シグナル伝達におけるヒスタミンH_2受容体のC末端の役割" 消化管ホルモン. XV. 42-45 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 菅野健太郎 他: "胃酸分泌機構" Arnural Review 消化器 1997. 68-74 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 菅野健太郎 他: "遺伝子操作によるヒスタミンH2受容体の構造と機能の解明" 消化器科. 24(4). 412-417 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 菅野健太郎: "DNA Sequence 法" G.I.Research. 5(4). 476-481 (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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