| Project/Area Number |
09470140
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | Miyazaki Medical College |
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Principal Investigator |
NAKAZATO Masamitsu Miyazaki Medical College, Third Department of Internal Medicine, Full time Lecturer, 医学部, 講師 (10180267)
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| Co-Investigator(Kenkyū-buntansha) |
MIYAZATO Mikiya National Cardiovascular Center Research Institute, Department of Biochemistry, Assistant Director, 生化学部, 室員 (50291183)
MATSUKURA Shigeru Miyazaki Medical College, Third Department of Internal Medicine, Professor, 医学部, 教授 (70030939)
加藤 丈司 宮崎医科大学, 医学部, 助手 (20274780)
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| Project Period (FY) |
1997 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥13,200,000 (Direct Cost: ¥13,200,000)
Fiscal Year 1999: ¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1998: ¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 1997: ¥7,400,000 (Direct Cost: ¥7,400,000)
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| Keywords | uroguanylin / guanylin / cyclic GMP / guanylate cyclase / salt and water metabolism / water-electrolyte balance |
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| Research Abstract |
Uroguanylin, a member of the guanylin peptide family, acts on guanylyl cyclase C (GC-C) to regulate intestinal and renal fluid and electrolyte transport through the second messenger, cyclic GMP. Using an antiserum raised against synthetic rat uroguanylin, we established a radioimmunoassay and identified three endogenous molecular forms in the intestine and kidney: a 15-amino acid uroguanylin, an 18-amino acid uroguanylin that is a monobasic processing product, and a 9.4-kDa prouroguanylin. Prouroguanylin is the major molecular form in these two tissues, whereas only 15-amino-acid uroguanylin is present in the urine. Rat uroguanylin is most abundant in the proximal small intestine, its content decreasing toward the colon. Uroguanylin is present immunohistochemically in the endocrine cells in the intestine and stomach, B cells in the pancreatic islets, and tubular epithelial cells in the kidney. Uroguanylin has a widespread tissue distribution and is located in cells which function in an endocrine, paracrine and/or immunocrine (luminal secretion) fashion. Uroguanylin may have physiological functions other than the regulation of fluid and electrolyte transport in the intestine and kidney.
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