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AnalysIs of molecular mechanism of gastric pre-cancerous lesions by using new mucin genes

Research Project

Project/Area Number 09470141
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Gastroenterology
Research InstitutionSapporo Medical University

Principal Investigator

IMAI Kohzoh  Sapporo Medical University, Prosesor, 医学部, 教授 (60117603)

Co-Investigator(Kenkyū-buntansha) ENDO Takao  Sapporo Medical University, Lecturer, 医学部, 講師 (40191928)
HINODA Yuji  Sapporo Medical University, Assistant Professor, 医学部, 助教授 (10165128)
ITOH Fumio  Sapporo Medical University, Lecturer, 医学部, 講師 (90223180)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 1998: ¥3,400,000 (Direct Cost: ¥3,400,000)
Keywordsmucin genes / gastric pre-cancerous lesions / Intestinal metaplasia / gastric cancer / Barett esohagus / 分子機構解析
Research Abstract

Our previous study showed that a new mucin antigen A3D4 was expressed at the protein level in gastric cancer and intestinal metaplasia associated with cancer, but not in normal gastric mucosa and intestinal metaplasia with chronic gastritis. To verify if the antigen is of use for diagnostic purpose, we performed an immunohistochemical study using a large number of gastric tissue specimens obtained by endoscopic biopsy. Antigen A3D4 was expressed in adenoma, especially intestinal type with high frequency and in intestinal metaplasia in the vicinity of adenoma. Of note, about 20% of intestinal metaplasia with chronic gastritis cases demonstrated to be positive for antigen A3D4, where only part of the deep portion of glands were faintly immunostained. These data suggest that antigen A3D4-positive intestinal metaplasia cases may be grouped into a high risk group for gastric adenoma and cancer.
It is known that MUC2 mucin is expressed in goblet cells of intestinal metaplasia, but not in normal gastric mucosa. We revealed that MUC2 was expressed in regenerative epithelia and gastric cancer, especially intestinal type, in addition to intestinal metaplasia. It will be important to examine the expression of MUC2 in the background mucosa of gastric cancer except for intestinal metaplasia. We then showed the expression of sulfo-Lewis A, which was shown expressed on MUC2 mucin core protein, in Barrette*s esophagus and esophageal adenocarcinoma by using a monoclonal antibody 91.9H.Thus, these two antigens, MUC2 and sulfo-Lewis A may be of use for analyzing the precancerous lesions of the stomach and esophagus, respectively.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] Shinoda,M.et al.: "Effective adoptive immunotherapy by T-LAK cells retargeted with bact-erial superantigen-conjugated antibody to MUC1 in xenografted SCID mice." Cancer Res.58. 2838-2843 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yoshida,Y.et al.: "Development of the arbitrarily primed-representational difference analysis(AP-RDA)method and chromosomal mapping of isolated high throughput rat genetic markers." Proc.Natl.Acad.Sci.USA. in press. (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Takaoka,AS.et al.: "Cloning and characterization of the human beta4-integrin gene promoter and enhancers." J.Biol.Chem.273. 33848-33856 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Takaoka,AS.et al.: "Suppression of invasive properties of colon cancer cells by a metastasis suppressor KAI1 gene." Oncogene.16. 1443-1453 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Suwa,T.et al.: "Increased invasiveness of MUC1 cDNA-transfected human gastric cancer MKN74 cells." Int.J.Cancer. 76. 377-382 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Endo,T.et al.: "Expression of sulfated carbohydrate chain and core peptides detect by monoclonal antibodies in Barrette s esophagus and esophageal adenocarcinoma."

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Akashi, H., et al.: "A novel gastric cancer-associated mucin antigen defined by a monoclonal antibody A3D4." Int.J.Cancer. 73. 795-801 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Suwa, T., et al.: "Increaced invasiveness of MUC1 cDNA-transfected human gastric cancer MKN74, cells." Int.J.Cancer. (in press). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yawata, A., et al.: "Prolonged cell suivival enhances peritoneal dissemination of gastric cancer cells." Oncogene. 16 (in press). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Adachi, T., et al.: "Increased sensitivity of gastric cancer cells to MK and LAK cells by antisense suppression of UD-GalNAc : polypeptide N-acetylgalacto-saminyl-transferase." J.Immunol.159. 2645-2651 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yamamoto, H., et al.: "Relation of enhanced secretion of active matrix metalloproteases with tumor spread in human hepatocellular carcinoma." Gastroenterology. 112. 1290-1296 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Takaoka, A., et al.: "Anti-cell death activity promotes pulmonary metastasis of melanoma cells." Oncogene. 14. 2971-2977 (1977)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Shinoda,M.et al.: "Effective adoptive immunotherapy by T-LAK cells retargeted with bacterial superantigen-conjugated antibody to MUC1 in xenografted SCID mice." Cancer Res.58. 2838-2843 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Yoshida,Y.et al.: "Development of the arbitrarily primed-representational difference analysis(AP-RDA)method and chromosomal mapping of isolated high throughput rat genetic markers" Proc.Natl.Acad.Sci.USA. in press. (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Takaoka,AS.et al.: "Cloning and characterization of the human beta4-integrin gene promoter and enhancers." J.Biol.Chem.273. 33848-33856 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Takaoka,AS.et al.: "Suppression of invasive properties of colon cancer cells by a metastasis suppressor KAI1 gene." Oncogene. 76. 1443-1453 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Suwa,T.et al.: "Increased invasiveness of MUC1 cDNA-transfected human gastric cancer MKN74 cells." Int.J.Cancer. 76. 377-382 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Endo,T.et al.: "Expression of sulfated carbohydrate chain and core peptides detected by monoclonal antibodies in Barrette s esophagus and esophageal adenocarcinoma." J.Gastroenterol.33. 811-815 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Akashi,H.,et al.: "A novel gastric cancer-associated mucin antigen defined by a monoclonal antibody A3D4." Int.J.Cancer. 73. 795-801 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Suwa,T.,et al.: "Increaced invasiveness of MUC1 cDNA-transfected human gastric cancer MKN74,cells." Int.J.Cancer. (in press). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yawata,A.,et al.: "Prolonged cell suivival enhances.peritoneal dissemination of gastric cancer cells." Oncogene. 16. in-press (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Adachi,T.,et al.: "Increased sensitivity of gastric cancer cells to NK and LAK cells by antisense suppression of UD-GalNAc:polypeptide N-acetylgalactosaminyl-transferase." J.Immunol.159. 2645-2651 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yamamoto,H.,et al.: "Relation of enhanced secretion of active matrix metalloproteases with tumor spread in human hepatocellular carcinoma." Gastroenterology. 112. 1290-1296 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Takaoka,A.,et al.: "Anti-cell death activity promotes pulmonary metastasis of melanoma cells." Oncogene. 14. 2971-2977 (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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