| Project/Area Number |
09470164
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| Research Category |
Grant-in-Aid for Scientific Research (B).
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Circulatory organs internal medicine
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| Research Institution | KYOTO UNIVERSITY (1998-2000)
Toyama Medical and Pharmaceutical University (1997) |
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Principal Investigator |
KISHIMOTO Chiharu Kyoto Univ, Dept.of Cardiovasc.Med, Med.Instructor, 医学研究科, 助手 (70169845)
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| Co-Investigator(Kenkyū-buntansha) |
YASUTOMI Yasuhiro Mie Univ, Dept. of Biological Reg, Professor, Associate Professor, 医学部, 助教授 (90281724)
KURIBAYASHI Kagemasa Mie Univ, Dept.of Biological Reg, Professor, 医学部, 教授 (10064578)
OCHIAI Hiroshi Toyama Med.Pharm.Univ.Human Sci. Fund. Nursing, Professor, 医学部, 教授 (30018692)
YOSHIDA Imanaka-kyoko Mie Univ, Dept. of Pathology, Instructor, 医学部, 講師 (00242967)
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| Project Period (FY) |
1997 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥8,700,000 (Direct Cost: ¥8,700,000)
Fiscal Year 2000: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1999: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1998: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1997: ¥6,100,000 (Direct Cost: ¥6,100,000)
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| Keywords | Viral myocarditis / Dilated cardiomyopathy / Coxsackievirus B3 / Apoptosis / Epitopes / Peptide therapy |
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| Research Abstract |
To clarify the pathogenesis and the development of coxsackievirus B3 (CVB3) myocarditis, we examined the histopathology of CVB3-inoculated wild type and severe combined immunodeficient C3H/He mice, and analyzed using subcloning method the sequence of nucleotides of an amyocarditic strain of CVB3 (CVBO) with comparison of that of a myocarditic strain of CVB3 (CVBM), which was already reported. Severe myocarditis developed in wild mice inoculated with CVBM.On the contrary, mild myocarditis resulted from wild mice inoculated with CVBO.In scid mice, severe myocarditis developed by the inoculation of both CVBM and CVBO.There were seven nucleotide differences with five corresponding amino acid changes in the sequence of CVBO in comparison with that of CVBM.The changes located in 1A, 1C, and 1D regions of viral genome, which encoded the envelope protein. Thus, antigenic determinant of CVB3 is related, at least in part, to the recognition of immunocompetent cells to the difference of nucleotides in these regions. In conclusion, the development of myocarditis is related to the immunological background of the host, and myocarditis is due, in part, to an immunopathogenic phenomenon.
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