| Project/Area Number |
09470198
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Radiation science
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| Research Institution | Fukui Medical University |
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Principal Investigator |
YONEKURA Yoshiharu Fukui Medical University, Biomedical Imaging Research Center, Professor, 高エネルギー医学研究センター, 教授 (60135572)
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| Co-Investigator(Kenkyū-buntansha) |
TSUCHIDA Tatsuro Fukui Medical University, Dept. of Radiology, Instructor, 医学部・附属病院, 助手 (70303386)
MURATA Tetsuhito Fukui Medical University, Dept. of Psychiatry, Instructor, 医学部, 助手 (80200294)
YASUHISA Fujibayashi Fukui Medical University, Biomedical Imaging Research Center, Professor, 高エネルギー医学研究センター, 教授 (50165411)
定藤 規弘 福井医科大学, 高エネルギー医学研究センター, 講師 (00273003)
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| Project Period (FY) |
1997 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥13,100,000 (Direct Cost: ¥13,100,000)
Fiscal Year 1999: ¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 1998: ¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 1997: ¥6,300,000 (Direct Cost: ¥6,300,000)
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| Keywords | dopamine / synaptic function / functional neuroimaging / PET / SPECT |
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| Research Abstract |
The aim of this study was to establish the methods for the quantitative analysis of dopaminergic synaptic function in human brain, basic an clinical sdudies were performed with various approach, including the kinetc analysis of SPECT imaging and basic experiments using fresh brain slices of rats. Serial dynamic SPECT imaging of I-123 iodobenzofuran (IBF) with intermittent arterial blood sampling permits to obtain binding potential of dopamine D2 receptors. In addition to the compartment analysis and simple target to nontarget ratio approach, we have developed a new graphical method which provides more reliable parameters on receptor binding. Dopamine transporter imaging was performed with I-123 fluoropropyl analogue of beta-CIT (FPCIT). FPCIT has an advantage of faster kinetics than beta-CIT, and test anr re-test brain SPECT scans demonstrated an excellent reproducibility of measuring the distribution volume in the striatum, suggesting that this tracer is promising for the clinical use. We also developed a system called dynamic positron autoradiography technique (dPAT) for serial imaging of fresh brain slices of rats with positron labeled radioligands. This new method permits the assessment of regional changes of radioactivity for several hours, and can be used to monitor glucose metabolism and receptor binding with various interventions, such as hypoxia and drug effects. It will play an important role in understanding the kinetic behaviour of radioligands.
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