| Project/Area Number |
09470226
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
内分泌・代謝学
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| Research Institution | University of Occupational and Environmental Health |
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Principal Investigator |
ETO Sumiya Scl.of Med.University of Occupational and Environmental Health Professor, 医学部, 教授 (90010347)
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| Co-Investigator(Kenkyū-buntansha) |
TUKADA Junichi Scl.of Med. University of Occupational and Environmental Health Lecturer, 医学部, 助手 (20227367)
MORIMOTO Isao Scl.of Med.University of Occupational and Environmental Health Associate Prof., 医学部, 助教授 (80145234)
TANAKA Yoshiya Scl.of Med.University of Occupational and Environmental Health Assist.Prof., 医学部, 講師 (30248562)
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| Project Period (FY) |
1997 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥8,200,000 (Direct Cost: ¥8,200,000)
Fiscal Year 1998: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1997: ¥5,200,000 (Direct Cost: ¥5,200,000)
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| Keywords | ATL / Hypercalcemia / Cell Adhesion Molecules / IL-1 gene / TAX protein / TAX蛋白 / 細胞接着 / LFA-1 / イソテグン / G蛋白 / Tax蛋白 / IL-1β遺伝子 / 核内転写因子 |
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| Research Abstract |
I.Results related with IL-1 (Bone resorption activating factor) gene Insertion of Human IL-1beta gene promotor fragmen with TAX protein which is producted by HTLV- I infection to Jurkat cells ; not HTLV-I infected T cell leukemia (ATL model) increased IL-1beta gene activity assayed by CAT compared with non- inserted cells. When the binding site in gene promortor of NF-IL6 or SP-1 which are the IL-1beta gene transactivators was muteted, this increse was completely blocked.
Insertion of Human IL-1alpha cx gene promotor fragment with TAX protein also incresed IL-1alpha a gene activity. In this case, this increase was blocked by the mutation of binding site of NF- Kbeta which is the IL-1alpha gene transactivator.
These results sugest that TAX protein is strongly involved with the production of IL-1, and the binding of NF-IL6, SP-1, NF-Kbeta to IL-1 gene promortor is essential for induction of IL-1 by TAX protein.
II.Result related with adhesion molecules
It is known that bone resorption is induced through osteoblast. Heterotypic adhesion of ATL cells and HOS cells ; osteoblast cell line, increased the expression of IL-1 mRNA in HOS cells and production of IL-1beta from HOS cells. In addition, this mRNA expression and protein production was significantly decreased by adding anti-VLA-4, LFA-1 antibody.
Theses results sugest that adhesion of ATL cells and osteoblast through various adhesion molecules is also essential for producton of IL-1.
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