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Activation of cytokine signaling by constitutively active STAT5.

Research Project

Project/Area Number 09470227
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Hematology
Research InstitutionThe University of Tokyo

Principal Investigator

KITAMURA Toshio  Institute of Medical Science, The University of Tokyo, Visiting Professor, 医科学研究所, 客員教授 (20282527)

Co-Investigator(Kenkyū-buntansha) ASANO Shigetaka  Institute of Medical Science, The University of Tokyo, Professor, 医科学研究所, 教授 (50134614)
IWAKURA Yoichiro  Institute of Medical Science, The University of Tokyo, Professor, 医科学研究所, 教授 (10089120)
NOSAKA Tetsuya  Institute of Medical Science, The University of Tokyo, Visiting Associate Professor, 医科学研究所, 客員助教授 (30218309)
Project Period (FY) 1997 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥11,700,000 (Direct Cost: ¥11,700,000)
Fiscal Year 1999: ¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 1998: ¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 1997: ¥5,500,000 (Direct Cost: ¥5,500,000)
KeywordsCytokine / Cytokine receptor / STAT5 / Transcription factor / Apotosis / Differentiation / Proliferation / IL-3 / サイトカインレセプター
Research Abstract

Cytokines have two major characteristics; functional redundancy of multiple cytokines and pleiotropic functions of a single cytokine. We previously presented the first evidence that a shared subunit of cytokine receptors is responsible for the functional redundancy of cytokines. On the other hand, the molecular basis of pleiotropic functions of cytokines has not been elucidated. Among signaling pathways stimulated by cytokine receptors, recently identified JAK-STAT pathway is now extensively studied. There are four JAK kinases and seven STAT transcription factors. Each STAT protein activates many target genes, which may explain. pleiotropic functions of cytokines. However, it has been difficult to identify the direct biological outcome of STAT activation because cytokine stimulation leads to simultaneous activation of many signaling pathways including the JAK-STAT pathway.
We were interested in STAT5 molecules because STAT5 is activated by a variety of cytokines with pleiotropic functio … More ns. To study direct biological outcomes of STAT5 activation, we attempted to identify a constitutively active STAT5 using PCR-driven random mutagenesis followed by retrovirus-mediated expression screening, and identified constitutively active forms of STAT5. The mutant STAT5 possessed constitutive tyrosine phosphorylation and DNA binding activity, induced expression of bcl-xL and pim-1 in the absence of IL-3 in Ba/F3 cells, and rendered Ba/F3 cells factor-independent. Unexpectedly, IL-3 treatment of the factor-independent Ba/F3 cells expressing the constitutively active STAT5 resulted in apoptosis within 24 hours, or differentiation followed by cell death. In these cells, mRNA expression of negative regulators downstream of STAT5 including CIS. JAB/SOCS-1/SSI-1, and p21ィイD1WAF1/Cip1ィエD1 were highly induced, and the expression of these negative regulators persisted for a and long period. Of the STAT5-regulated genes, constitutive expression of JAB/SOCS-1/SSI-1 was sufficient to induce apoptosis of Ba/F3 cells, while p21ィイD1WAF1/Cip1ィエD1 induced macrophage differentiation of these cells. Constitutive expression of pim-1 was sufficient to induce IL-3-independent growth of Ba/F3 cells. In addition, the constitutively active STAT5 induced macrophage differentiation of M1 leukemic cells, where STAT5-driven IL-6 production induced M1 differentiation through a autocrine fashion. These findings suggest that a single transcription factor regulates cell fate by varying the intensity and duration of the expression of a set of target genes. Less

Report

(4 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • 1997 Annual Research Report
  • Research Products

    (95 results)

All Other

All Publications (95 results)

  • [Publications] Kawakami, Y: "Bruton's tyrosine kinase ( Btk) regulates the JNK/ SAPK kinase activity."Proc. Natl, Acad, Sci,U. S. A. 94. 3938-3942 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Ito, K: "Development of a novel selective amplifier gene for controlling expansion of transduced hematopoietic cell."Blood. 90. 3884-3892 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Onishi, M.: "Cytokine receptors: Structures and signal transduction. Int"Rev. Immunol. 16. 617-634 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kitamura, T.: "New experimental approaches in retrovirus- mediated expression screening."Int. J. Hematol.. 67. 351-359 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Hata,D.: "Involvement of Bruton' s tyrosine kinase in FceRI- dependent mast cell degranulation and cytokine production."J. Exp.Med.. 187. 1235-1247 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Morikawa, Y.: "Induction of synaptosomal- associated protein-23kd (SNAP-23) by various cytokines."Blood. 92. 129-135 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Onishi, M.: "Identification and characterization of a constitutive active STAT5 mutant that promotes cell proliferation."Mol. Cell. Biol.. 18. 3871-3879 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Jinno, A: "Identification of the chemokine receptor TER1/ CCR8 expressed in brain- derived cells and T- cells as a new coreceptor for HIV- 1 infection."Biochem. Biophys. Res. Commun.. 243. 497-502 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kawashima, T.: "Interleukin-12 induces tyrosine phosphorylation of an 85-kDa protein associated with the interleukin-12 receptor_1 subunit."Cell. Immunol.. 186. 39-44 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kitamura, T.: "Activating mutations of the transmembrane domain of MPL in vitro and in vivo; Incorrect sequence of MPL_K, an alternative spliced form of MPL."Blood. 92. 2596-2597 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Dairaghi, D.J.: "RANTES-induced activation correlates with CD3 expression."J.Immunol. 168. 426-433 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Duan, Y.: "Expression and functional analysis of a hemopoietic progenitor antigen,NJ- 1( 114/ A10)."Biochem. Biophys. Res. Commun.. 253. 401-406 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yang,Y-L: "Receptors for polytropic and xenotropic murine leukemia viruses encoded by a single gene at Rmc1."Nature Genetics. 21. 216-219 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Nosaka, T.: "Identification and characterization of constitutively active STAT5"Molecular Biology of Hematopoiesis 6 (Abraham N. G. et al. eds.Kluwer Academic/ Plenum Publishers, New York). 277-287 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Mukasa, R.: "Core 2- containing O- glycans on CD43 are preferentially expressed in the memory subset of human CD4 T cells."Int. Immunol.. 11. 259-268 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Matsumura, L: "Involvement of cyclin D1 in STAT5- mediated cell growth."EMBOJ.. 18. 1367-1377 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Nieborowska-Skorska, M.: "Signal transducer and activator of transcription (STAT) 5 activation by BCR/ ABL is dependent on intact Src homology (SH) 3 and SH2 domains of BCR/ABL and is required for leukemogenesis."J. Exp.Med.. 189. 1229-1242 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kojima, T.: "A novel signal sequence trap method using retrovirus-mediated gene transfer"Nature Biotechnol.. 17. 487-490 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yagisawa, M.: "Signal transduction pathways in normal human moncytes stimulated by cytokines and mediators comparative study with normal human neutrophils or transformed cells and the putative roles in functionality and cell biology."Exp. Hematol.. 27. 1063-1076 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Shimizu, N.: "An orphan G protein coupled receptor, GPR1, act as a determinant co-receptor allowing replication of human immunodeficiency virus type 1 and type 2 in brain-derived cells."J.Virol.. 258. 5231-5239 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Soda, Y.: "Establishment of a new system for determination of coreceptor usages of HIV based on the human glioma NP-2 cell line."Biochem. Biophys. Res. Commun.. 258. 313-321 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Nosaka, T.: "STAT5 as a molecular regulator of proliferation, differentiation, and apoptosis in hematopoietic cells."EMBOJ.. 18. 4754-4765 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Ye, S. K.: "Induction of germline transcription in the TCRg locus by STAT5: implications for accessibility control by the IL-7 receptor."Immunity. 11. 213-223 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kubota, N.: "PPAR_ mediates high-fat diet-induced adipocyte hypertrophy and insulin resistance."Mollecular Cell. 4. 597-609 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Tsukamoto, T.: "Isolation of oncogenes from rat mammary tumors by ahighly efficient retrovirus expression cloning system."Biochem.Biophys. Res. Commun. 265. 7-12 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yamada, K.: "Constitutively active STAT5 A and STAT5B in vitro and in vivo: mutation of STAT5 is not a frequent cause of leukemogenesis."Int. J. Hematol.. 71. 46-54 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kitamura, T.: "Isolation of cDNAs for T cell antigens by retrovirs-mediated expression cloning."T Cell Protocols, Methods in Molecular Biology.(Kearse,K.P ed. Humana Press, Totowa, New Jersey). 2000. 152 (143)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Hayakawa, F.: "Tandem-duplicated Flt3 constitutively activates STAT5 and MAP kinase and introduces autonomous cell growth in IL-3 dependent acute myeloid cell lines."Leukemia. (in press). (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Zhao, M.: "In vivo treatment of mutant FLT-3 transformed murine leukemia with a tyrosine kinase inhibitor"Oncogene. (in press). (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Nosaka, T.: "Jaks and STATs in hematopoietic cells."Int. J. Hematol.. (in press). (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Fujio, K.: "Molecular cloning of a novel type I cytokine receptor."Blood. (in press). (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Misawa, K: "Molecular cloning and tissue distribution of a mouse homologue of human LIGHT, a member of TNF super family."Cytogenetics and Cell Genetics. (in press). (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Misawa, K: "A novel method to identify cDNAs based on Iocalization of the GFP-fusion products."Proc. Natl, Acad, Sci,U. S. A. (in press). (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Morita, S.: "An efficient and stable retrovirus packaging system for transient packaging."Gene Therapy. (in press). (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kawakami, Y., Miura, T., Bissonette, R., Hata, D., Khan, W.N., Kitamura, T., Maeda-Yamamoto, M., Hartman, S.E., Yao, L., Alt, F.W., and Kawakami, T.: "Bruton's tyrosine kinase (Btk) regulates the JNK/SAPK kinase activity."Proc. Natl. Acad. Sci. U.S.A. 94. 3938-3942 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Ito, K., Ueda, Y., Kokubun, M., Urabe, M., Inaba, T., Mano, H., Hamada, H., Kitamura, T., Mizoguchi, H., Sakata, T., Hasegawa, M., and Ozawa, K.: "Development of a novel selective amplifier gene for controllable expansion of transduced hematopoietic cells."Blood. 90. 3884-3892 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Onishi, M., Nosaka, T., and Kitamura, T.: "Cytokine receptors: Structures and signal transduction."Int. Rev. Immunol.. 16. 617-634 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kitamura, T.: "New experimental approaches in retrovirus-mediated expression screening."Int. J. Hematol.. 67. 351-359 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Hata, D., Kawakami, Y., Inagaki, N., Lantz, C.S., Kitamura, T., Khan, W.N., Tashiro, M., Maeda-Yamamoto, M., Miura, T., Han, W., Hartman, S.E., Yao, L., Nagai, H., Goldfeld, A.E., Alt FW., Galli, S.J., Witte, O.N., and Kawakami. T.: "Involvement of Bruton's tyrosine kinase in FceRI-dependent mast cell degranulation and cytokine production."J. Exp. Med.. 187. 1235-1247 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Morikawa, Y., Nishida, H., Misawa, K., Nosaka, T., Miyajima, A., Senba, E., and Kitamura, T.: "Induction of synaptosomal-associated protein-23kd (SNAP-23) by various cytokines."Blood. 92. 129-135 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Onishi, M., Nosaka, T., Misawa, K., Mui, A.L.-F., Gorman, D., McMahon, M., Miyajima, A., and Kitamura, T.: "Identification and characterization of a constitutive active STAT5 mutant that promotes cell proliferation."Mol. Cell. Biol.. 18. 3871-3879 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Jinno, A., Shimizu, N., Soda, Y., Haraguchi, Y., Kitamura, T., and Hoshino, H.: "Identification of the chemokine receptor TER1/CCR8 expressed in brain-derived cells and T-cells as a new coreceptor for HIV-1 infection."Biochem. Biophys. Res. Commun.. 243. 497-502 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kawashima, T., Kawasaki, H., Kitamura, T., Nojima, Y., and Morimoto, C.: "Interleukiu-12 induces tyrosipe phosphorylation of an 85-kDa protein associated with the interleukin-12 receptor_1 subunit."Cell. Immunol.. 186. 39-44 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kitamura, T., Onishi, M., Yahata, T., Kanakura, Y., and Asano, S.: "Activating mutations of the transmembrane domain of MPL in vitro and in vivo : Incorrect sequence of MPL-K, an alternative spliced form of MPL."Blood. 92. 2596-2597 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Dairaghi, D. J., Premack, B. A., Kitamura, T., Soo, K. S., Bacon, K. B., and Schall, T. J.: "RANTES-induced activation correlates with CD3 expression."J. Immunol.. 168. 426-433 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Duan, Y., Naruse, T., Kawashima, T., Morikawa, Y., Yamaguchi, Y., Nakamura, M., Kitamura, T., and Suda, T.: "Expression and functional analysis of a hemopoietic progenitor antigen, NJ-1 (1 14/A10), in a megakaryocytic lineage."Biochem. Biophys. Res. Commun.. 253. 401-406 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yang, Y-L., Lei, G., Xu, S., Holland, C. A., Kitamura, T., Hunter, K., and Cunningham, J. M.: "Receptors for polytropic and xenotropic murine leukemia viruses encoded by a single gene at Rmc1."Nature Genetics. 21. 216-219 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Nosaka, T., Onishi, M., Kawashima, T., Yamada, K., Misawa. K., Ariyoshi, K., Towatari, K., Saito, H., Tani, K., Asano, S., Miyajima, A., and Kitamura, T.: "Identification and characterization of constitutively active STAT5."Molecular Biology of Hematopoiesis, 6, (Abraham N. G. et al. eds. Kluwer Academic/Plenum Publishers, New York). 277-287 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Mukasa, R., Homma, T., Ohtsuki, T., Hosono, O., Souta, A., Kitamura, T., Fukuda, M., Watanabe, S., and Morimoto, C.: "Core 2-containing O-glycans on CD43 are preferentially expressed in the memory subset of human CD4 T cells."Int. Immunol.. 11. 259-268 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Matsumura, I., Kitamura, T., Wakao, H., Hashimoto, K., Albanese, C., Downward, J., Pestell, R. G., and Kanakura, Y.: "Involvement of cyclin D1 in STAT5-mediated cell growth."EMBO J.. 18. 1367-1377 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Nieborowska-Skorska, M., Wasik, M. A., Slupianek, A., Salomoni, P., Kitamura, T., Calabretta, B., and Skorski, T.: "Signal transducer and activator of transcription (STAT) 5 activation by BCR/AJBL is dependent on intact Src homology (SH) 3 and SH2 domains of BCR/ABL and is required for leukemogenesis."J. Exp. Med.. 189. 1229-1242 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kojima, T., and Kitamura, T.: "A novel signal sequence trap method using retrovirus-mediated gene transfer."Nature Biotechnol.. 17. 487-490 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yagisawa, M., Saeki, K., Okuma, E., Kitamura, T., Kitagawa, S., Hirai, H., Yazaki, Y., Takaku, F., and You, A.: "Signal transduction pathways in normal human moncytes stimulated by cytokines and mediators : comparative study with normal human neutrophils or transformed cells and the putative roles in functionality and cell biology."Exp. Hematol.. 27. 1063-1076 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Shimizu. N., Soda, Y., Kanbe, K., Jinno, A., Kitamura, T., and Hoshino, H.: "An orphan G protein-coupled receptor, GPR1, act as a determinant co-receptor allowing replication of human immunodeficiency virus type 1 and type 2 in brain-derived cells."J. Virol.. 258. 5231-5239 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Soda, Y., Shimizu, N., Jinno, A., Liu, HY., Kanbe, K., Kitamura, T., and Hoshino, H.: "Establishment of a new system for determination of coreceptor usages of HIV based on the human glioma NP-2 cell line."Biochem. Biophys. Res. Commun.. 258. 313-321 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Nosaka, T., Kawashima, T., Misawa, K., Ikuta, K., Mui, A. L. F., and Kitamura, T.: "STAT5 as a molecular regulator of proliferation, differentiation, and apoptosis in hematopoietic cells."EMBO J.. 18. 4754-4765 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Ye, S. K., Maki, K., Kitamura, T., Sunaga, S., Akashi, K., Domen, J., Weissman, I. L., Honjo, T., and Ikuta, K.: "Induction of germline transcription in the TCRg locus by Stat5 : implications for accessibility control by the IL-7 receptor."Immunity. 11. 213-223 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kubota, N., Terauchi, Y., Miki, H., Tamemoto, H., Yamauchi, T., Komeda, K., Satoh, S., Nakano, R., Ishii, C., Sugiyama, T., Eto, K., Tsubamoto, Y., Okuno, A., Murakami, K., Sekihara, Hi., Hasegawa, G., Naito, M., Toyashima, Y., Tanaka, S., Shiota, K., Kitamura, T., Fujita, T., Ezaki, O., Aizawa, S., Nagai, R., Tobe, K., Kimura, S., and Kadowaki, T.: "PPARγ mediates high-fat diet-induced adipocyte hypertrophy and insulin resistance."Mollecular Cell. 4. 597-609 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Tsukamoto, T., Huang, T., Guzman, R. C., Chen, X., Pascual, R. V., Kitamura, T., and Nandi, S.: "Isolation of oncogenes from rat mammary tumors by a highly efficient retrovirus expression cloning system."Biochem. Biophys. Res. Commun.. 265. 7-12 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yamada, K., Ariyoshi, K., Onishi, M., Miyajima, A., Hayakawa, F., Towatari, M., Saito, H., Oka, Y., Asano, S., Nosaka, T., and Kitamura, T.: "Constitutively active STAT5A and STAT5B in vitro and in vivo : mutation of STAT5 is not a frequent cause of leukemogenesis."Int. J. Hematol.. 71. 46-54 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kitamura, T., and Morikawa, Y.: "Isolation of cDNAs for T cell antigens by retrovirs-mediated expression cloning."T Cell Protocols, Methods in Molecular Biology. (Kearse, KP. ed. Humana press, Totowa New Jersey). 143-152 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Hayakawa, F,. Towatari, M., Kiyoi, H., Tsuzuki, S., Iida, H., Kitamura, T., Saito, H., and Naoe, T.: "Tandem-duplicated Flt3 constitutively activates STAT5 and MAP kinase and introduces autonomous cell growth in IL-3 dependent acute myeloid cell lines."Leukemia. (in press). (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Zhao, M., Kiyoi, H., Yamamoto, Y., Ito, M., Towatari, M., Ohmura, S., Kitamura, T., Ueda, R., Saito, H., and Naoe, T.: "In vivo treatment of mutant FLT-3-transformed murine lenkemia with a tyrosine kinase inhibitor."Oncogene. (in press). (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Nosaka, T., and Kitamura, T.: "Jaks and STATs in hematopoietic cells."Int. J. Hematol.. (in press). (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Fujio, K., Nosaka, T., Kojima, T., Kawashima, T., Yahata, T., Copeland, N. G., Gilbert, D. J., Jenkins, N. A., Yamamoto, K., Nishimura, T., and Kitamura, T.: "Molecular cloning of a novel type I cytokine receptor."Blood. (in press). (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Misawa, K., Nosaka, T., Kojima, T., Hirai, M., and Kitamura, T.: "Molecular cloning and tissue distribution of a mouse homologue of human LIGHT, a member of TNF super family."Cytogenetics and Cell Genetics. (in press). (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Misawa, K., Morita. S., Nosaka, T., Kaneko. A., Nakahata, T., Asano, S., and Kitamura, T.: "A method to identify cDNAs based on localization of the green fluorescent protein-fusion products."Proc. Natl. Acad. Sci. U.S.A.. (in press). (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Morita, S., Kojima, T., and Kitamura, T.: "An efficient and stable retrovirus packaging system for transient packaging."Gene Therapy. (in press). (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Nosaka, T.: "Identification and characterization of constitutively active STAT5"Molecular Biology of Hematopoiesis 6. 277-287 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Matsumura, I.: "Involvement of cyclin D1 in STAT5-mediated cell growth"EMBO J.. 18. 1367-1377 (1999)

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      1999 Annual Research Report
  • [Publications] Nieborowska-Skorska, M.: "Signal transducer and activator of transcription (STAT) 5 activation by BCR/ABL is depedent on intact Src homology (SH) 3 and SH2 domains of BCR/ABL and is required for leukemogenesis"J. Exp. Med.. 189. 1229-1242 (1999)

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      1999 Annual Research Report
  • [Publications] Nosaka, T.: "STAT5 as a molecular regulator proliferation, differentiation, and apoptosis in hematopoietic cells"EMBO J.. 18. 4754-4765 (1999)

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      1999 Annual Research Report
  • [Publications] Yamada, K.: "Constitutively active STAT5A and STAT5B in vitro and in vivo : mutation of STAT5 is not a frequent cause of leukemogenesis"Int. J. Hematol.. 71. 46-54 (2000)

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      1999 Annual Research Report
  • [Publications] Nosaka, T.: "Jaks and STATs in hematopoietic cells"Int. J. Hematol.. (in press.). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Morita, S.: "An efficient and stable retrovirus packaging sysytem for transient packaging"Gene Therapy. (in press). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Onishi,M.: "Cytokine receptors:Structures and signal transduction." Int.Rev.Immunol.16. 617-634 (1998)

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  • [Publications] Kitamura,T.: "New experimental approaches in retrovirus-mediated expression screening." Int.J.Hematol.67. 351-359 (1998)

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  • [Publications] Hata,D.: "Involvement of Bruton's tyrosine kinase in FceRI-dependent mast cell degranulation and cytokine production." J.Exp.Med.187. 1235-1247 (1998)

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  • [Publications] Morikawa,Y.: "Induction of synaptosomal-associated protein-23kd(SNAP-23)by various cytokines." Blood. 92. 129-135 (1998)

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      1998 Annual Research Report
  • [Publications] Onishi,M.: "Identification and characterization of a constitutive active STAT5 mutant that promotes cell proliferation." Mol.Cell.Biol.18. 3871-3879 (1998)

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  • [Publications] Jinno,A.: "Identification of the chemokine receptor TER1/CCR8 expressed in brain-derived cells and T-cells as a new coreceptor for HIV-1 infection." Biophys.Biochem.Res.Commun.243. 497-502 (1998)

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  • [Publications] Kawashima,T.: "Interleukin-12 induces tyrosine phosphorylation of an 85-kDa protein associated with the interleukin-12 receptor β1 subunit." Cell.Immunol.186. 39-44 (1998)

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  • [Publications] Kitamura,T.: "Activating mutations of the transmembrane domain of MPL in vitro and in vivo:Incorrect sequence of MPL-K,an alternative spliced form of MPL." Blood. 92. 2596-2597 (1998)

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  • [Publications] Dairaghi,D.J.: "RANTES-induced activation correlates with CD3 expression." J.Immunol.168. 426-433 (1998)

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  • [Publications] Duan,Y.: "Expression functional analysis of a hemopoietic progenitor antigen,NJ-1(114/A10),in a megakaryocytic lineage." Biochem.Biopys.Res.Commun.253. 401-406 (1998)

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  • [Publications] Kitamura,T.: "Isolation of cDNAs for T cell antigens by retrovirs-mediated expression cloning." Methods in Molecular Biology.(in press). (1999)

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      1998 Annual Research Report
  • [Publications] Nosaka,T.: "Identification and characterization of constitutively active STAT5." Molecular Biology of Hematopoiesis. (in press). (1999)

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  • [Publications] Mukasa,R.: "Core 2-containing O-glycans on CD43 are preferentially expressed in the memory subset of human CD4 T cells." Int.Immunol. (in press). (1999)

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  • [Publications] Matsumura,I.: "Involvement of cyclin D1 in STAT5-mediated cell growth." EMBO J.(in press). (1999)

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  • [Publications] Onishi,M.et al.: "Cytokine receptors:Structures and signal transdaction" Int.Rev.Immunel.(in press). (1998)

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  • [Publications] Kitamura,T.: "New experimental approaches using iretrovirus-mediated expression screening" Int.J.Hematol.(in press). (1998)

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  • [Publications] Kawakami,Y.et al.: "Bruton's tyrosine kinase (Bck) regalates the JNK/SAPK kinase activity." Proc.Natl.Acad.Sci.U.S.A.Vol.94. 3938-3942 (1997)

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  • [Publications] Ito,K.et al.: "Development of a novel selective amplifier gene for controllable expansion of transduced hematopoietic cells." Blood. Vol.90. 3884-3892 (1997)

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  • [Publications] Hata,D.et al.: "Involvement of Bruton's tyrosine kinase in FceRI-dependent miase cell degranalation and cytokine production" J.Exp.Med.(in press). (1998)

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  • [Publications] Morikawa,Y.et al.: "Induction of synaptosomal-associated protein-23kd(SNAP-23) by various cytokines." Blood. (in press). (1998)

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Published: 1997-04-01   Modified: 2016-04-21  

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