| Project/Area Number |
09470237
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Kidney internal medicine
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| Research Institution | Niigata University |
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Principal Investigator |
YAMAMOTO Tadashi Niigata University, School of Medicine ; Professor., 医学部, 教授 (30092737)
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| Co-Investigator(Kenkyū-buntansha) |
KAWASAKI Katsutoshi School of Medicine ; Niigata University Lecturer., 医学部, 講師 (20152944)
KIHARA Itaru School of Medicine ; Niigata University Professor., 医学部, 教授 (80018324)
YAOITA Eishin School of Medicine ; Niigata University Associate Professor., 医学部, 助教授 (00157950)
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| Project Period (FY) |
1997 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥8,700,000 (Direct Cost: ¥8,700,000)
Fiscal Year 1999: ¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 1998: ¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1997: ¥3,900,000 (Direct Cost: ¥3,900,000)
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| Keywords | Glomerulus / Differential screening / Structure-function correlation / Ribonuclease protection assay / In situ hybridization / 構造-機能連間 / リポヌクレアーゼプロテクション法 / インサイチュ-ハイプリダイゼーション / 糸球体上皮細胞 / 遺伝子 / ライブラリー / ゴルジ装置 / 糸球体腎炎 |
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| Research Abstract |
The unique function of renal glomerulus should be related to its unique structure and molecular components. To understand the structural and functional characteristics of renal glomerulus we first aimed to identify molecules which were specifically present in this structure. We employed differential screening methods to isolate genes which were predominantly expressed in rat glomerulus. Several genes have been isolated as those which were predominantly or uniquely expressed in the glomerulus although their sequences had been registered : olfactomedin-related protein, thymosin β4, heparin-binding epidermal growth factor, VE-cadherin, βCOP and collagen type IV α3 chain. A few novel genes were also isolated as glomerulus-prone ones : 14-1 and 17-3. In addition, several novel genes were identifed as kidney-specific or kidney-prone ones : Ig-Hepta, FR-67 and CS-5. Among them, olfactomedin-related protein and Ig-Hepta were intensively studied. Olfactomedin-related protein was localized in the Golgi apparatus of glomerular podocytes and its C-terminus amino acid sequence, SDEL was a Golgi-retension signal. Ig-Hepta had two Ig-like domains and 7 transmembrane domains. The molecule was immunolocalized in the intercalated cells in the collecting duccts, suggesting a role in acid-base balance regulation.
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