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The Mechanism of Stimulus Secretion Coupling in the Endocrine Tumors

Research Project

Project/Area Number 09470248
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field General surgery
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

ONODERA Hisashi (1998-1999)  Kyoto University, Graduate School of Medicine, Lecturer, 医学研究科, 講師 (50240825)

小切 匡史 (1997)  京都大学, 医学研究科, 助手 (60283595)

Co-Investigator(Kenkyū-buntansha) INOUE Kazutomo  Kyoto University, Institute for Frontier Medical Sciences, Professor, 再生医科学研究所, 教授 (90168435)
HOSOTANI Ryo  Kyoto University, Graduate School of Medicine, Lecturer, 医学研究科, 助教授 (00139908)
IMAMURA Masayuki  Kyoto University, Graduate School of Medicine, Professor, 医学研究科, 教授 (00108995)
SHIMADA Yutaka  Kyoto University, Graduate School of Medicine, Lecturer, 医学研究科, 講師 (30216072)
Project Period (FY) 1997 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥8,500,000 (Direct Cost: ¥8,500,000)
Fiscal Year 1999: ¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1998: ¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 1997: ¥3,400,000 (Direct Cost: ¥3,400,000)
Keywordspancreas / endocrine tumor / calcium / secretin / calcium-sensing receptor / カルシュウム / ホルモン分泌 / 細胞株 / 増殖因子
Research Abstract

1) We investigated the mechanism of hormone release from the pancreatic endocrine tumors. We already found that the insulin release from insulinoma cells are induced by the elevation of intracellular calcium concentration ([CaィイD12+ィエD1]i) and this [CaィイD12+ィエD1]i elevation is stimulated by the increase of extracellular calcium concentration ([CaィイD12+ィエD1]o). Therefore we paid attention and examined the expression of calcium-sensing receptor (CaR) in the insulinoma. Using electrophysioligical study, Southern blot analysis and immuno-histochemistry, it was proved that the CaR is expressed in insulinoma, gastrinoma and also in the normal islets. However, the responses of the CaR to calcium in endocrine tumors were different from the responses in the normal islets. We think this difference between the endocrine tumors and the normal islets plays an important role in the abnormality of hormone release in the endocrine tumors. Now, we further examines the function of CaR in the endocrine tumors.
2) It was confirmed that the human normal antral G cells and gastrinoma express the secretin receptor by RT-PCR. We are presently making the anti-secretin-receptor antibody to investigate the distribution of secretin receptor in the pancreatic endocrine tumors.
3) Concerning the culture and passage of endocrine tumor cells, in preliminary study, we succeeded in long term culture of normal islet cells using the chitosan mesh.

Report

(4 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • 1997 Annual Research Report

Research Products

(23 results)

All Other

All Publications (23 results)

  • [Publications] Hosotani,Ryo: "Surgical management of endocrine tumor of the pancreas in Japan"Journal of Hepato-Biliary-Pancreatic Surgery. 4 ・ 3. 295-303 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 小切匡史: "消化管ホルモン産出腫瘍"ホルモンと臨床. 45・10. 989-996 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Imamura,Masayuki: "Progress in the field of pancreatic endocrine tumors in Japan"Pancreas. 16. 379-384 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Imamura,Masayuki et al.: "Rationale of so-called extended resection for pancreatic invasive ductal carcinoma"Digestion. 60. 126-129 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 細谷亮: "肝臓手術の新しいテクニック:SASItestとIOStestによるガストリノーマ根治術"外科. 61. 31-36 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Hosotani, Ryo: "Surgical Management of Endocrine Tumor of the Pancreas in Japan"Journal of Hepato-Biliary-Pancreatic Surgery. 4/3. 295-303 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Imamura, Masayuki: "Progress in the Field of Pancreatic Endocrine Tumors in Japan"Pancreas. 16. 379-384 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Imamura, Masayuki: "Rationale of So-called Extended Resection for Pancreatic Invasive Ductal Carcinoma"Digestion. 60 (Supple 1). 31-36 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 加藤雅之 ら: "ガストリノーマの診断と治療指針"胆胆膵. 38・6. 975-980 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 加藤雅之 ら: "膵内分泌腫瘍の診断と治療"医学と薬学. 42・3. 373-381 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Masayuki Kato et al: "Curative vesection of microgasbyinomas based on introoperative secretion test"World Journal of Surgery. in press. (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Masayuki Imamura et al.: "Rationale of the so-called extended resection for pancreatic invisire cluctol carcinoma"Digestion. 60(suppl 1). 126-129 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Kogi Fugimoto et al: "Induction of all-cycle arrest and apoptosls by a novel retinobenzoic-acid derivative TAC-101"International Journal of Cancer. 81. 637-649 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 細谷亮ら: "膵臓手術の新しいテクニック:SASI test とLOS test によるガストリノーマで根治術"外科. 61. 31-36 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Masayuki Imamura: "Progress in the field of pancratic endocrino tumors in Japan" Pancreas. 16. 379-384 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Gakuji Ohshio: "Gastrinoma with unbtiple liver metastosesi effectiveness of DTICtlecy" J Hepto Biliary Pancreat. Surg.5. 339-343 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Masayuki Kato: "Response of human insediuma cells to extracellular calcium is different frow normal Base" Dig.Dis.Sci.43・11. 2429-2438 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 加藤,雅之: "選択的動脈内セクレチン注入試験によるガストリノーマの診断" 胆と膵. 20・2. 139-142 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Masayuki Kato: "Characterization of six cell lines established from human paravatic adenocation" Cancer. 85. 832-840 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Pyo Hosotani: "Surgical management of endocrine tumor of the pancreas in Japan" Journal of Hepato-Biliary-Pancreatic Surgery. 4・3. 295-303 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 小切匡史: "消化管ホルモン産生腫瘍" ホルモンと臨床. 45・10. 989-996 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Masayuki Kato: "Extracellular calcium sensing-receptors express in human insulinoma cells" Gastroenterology. 112・4. A451-A451 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 小切匡史: "外科手術のコツと落とし穴『内分泌外科』(分担執筆)" 文光堂(発表予定),

    • Related Report
      1997 Annual Research Report

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Published: 1997-03-31   Modified: 2016-04-21  

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