| Project/Area Number |
09470252
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| Research Category |
Grant-in-Aid for Scientific Research (B).
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | THE UNIVERSITY OF TOKUSHIMA |
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Principal Investigator |
TASHIRO Seiki SCHOOL OF MEDICINE, PROFESSOR, 医学部, 教授 (30040249)
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| Co-Investigator(Kenkyū-buntansha) |
HARADA Masamitu SCHOOL OF MEDICINE, ASISTANT, 医学部・附属病院, 助手 (60263828)
YOGITA Sirou SCHOOL OF MEDICINE, ASISTANT PROFESSOR, 医学部・附属病院, 講師 (40166864)
HIMENO Kunisuke SCHOOL OF MEDICINE, PROFESSOR, 医学部, 教授 (50112339)
ANDOH Tsutomu SCHOOL OF MEDICINE, ASISTANT, 医学部, 助手 (70304508)
MIYAKE Hidenori SCHOOL OF MEDICINE, ASISTANT, 医学部, 助手 (60274213)
福田 洋 徳島大学, 医学部・附属病院, 助手 (50274212)
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| Project Period (FY) |
1997 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥12,900,000 (Direct Cost: ¥12,900,000)
Fiscal Year 2000: ¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1999: ¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 1998: ¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 1997: ¥4,900,000 (Direct Cost: ¥4,900,000)
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| Keywords | small bowel transplantation / liver transplantation / immuno-tolerance / heat shock protein / ischemic-reperfusion injury / anti-apoptosis action / 免疫寛客 / 熱ショック蛋白質 / 免疫寛容 / microchimerism / ドナー脾細胞 / GVHD / リンパ球混合培養試験 / FK506 / ドナー抗原 |
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| Research Abstract |
We had three execution plan trough the 1997-2000 year.
1. In regard to microchimerism induction study, combined administration of the donor splenocytes and FK506 with the short periadministration. reduced allograft rejection and prolonged survival in the rat model of small bowel transplantation.
2. In regard to studies about anti-apoptosis action of non-toxic HSP 70 inducer, geranylgeranylacetone (GGA), GGA administration induced HSP 70 on hepatocytes exposed to hydrogen peroxide (H202) or ethanol and exerted anti-apoptosis action. These results suggest that GGA may have a potential benefit for the treatment of liver injury. Next, we examined the effects of GGA on acute hepatic injury after 95% hepatectomy in rats. All of 25 rats with vehicle died within 60 hours after 95% hepatictomy, while 10 Of 25 rats with GGA survived. And GGA significantly suppressed the release of transaminases and IL-6 and completely inhibited TNF-α production. The examination is being carried out in the ischemic liver transplantation model at present.
3. In this study, we examined the effect of FK506 and OKY046 (Tromboxane Synthase I nhibitor) against warm ischemia-reperfusion injury on non-heart-beating donor (NHBD) in orthotopic liver transplantation in rats. The 14 days survival rate in FK506 and OKY046 combined group was 100%, 83% in FK506 group, 83% in OKY046 group, compared to only 33% in control group. And serum AST, ALT, LDH, IL6 and TNF-α concentration after reperfusion were significantly improved in FK506 and OKY046 combined group, in FK506 group and in OKY046 group.
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