| Project/Area Number |
09470275
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Thoracic surgery
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| Research Institution | University of Tokyo |
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Principal Investigator |
ENDOH Hunemoto (1999) Faculty of Medicine, University of Tokyo, Assistant, 医学部・附属病院, 助手 (30312309)
川内 基裕 (1997-1998) 東京大学, 医学部・附属病院, 講師 (00152918)
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| Co-Investigator(Kenkyū-buntansha) |
OKA Teruaki Faculty of Medicine, University of Tokyo, Lecturer., 大学院・医学系研究科・医学部, 講師 (60177029)
TAKEDA Makoto Faculty of Medicine, University of Tokyo, Assistant, 医学部・附属病院, 助手 (10236482)
NAKAJIMA Jun Faculty of Medicine, University of Tokyo, Lecturer., 医学部・附属病院, 講師 (90188954)
竹下 美香 東京大学, 医学部・附属病院, 助手
宮地 鑑 東京大学, 医学部・附属病院, 助手 (40281703)
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| Project Period (FY) |
1997 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥12,500,000 (Direct Cost: ¥12,500,000)
Fiscal Year 1999: ¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 1998: ¥2,700,000 (Direct Cost: ¥2,700,000)
Fiscal Year 1997: ¥6,500,000 (Direct Cost: ¥6,500,000)
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| Keywords | xeno transplantation / Heart Transplantation / Rejection / HGF / VEGF / VEGF / Discordant異種移植 / ブタ / ニホンザル / 超急性拒絶反応 / Tunel染色 / 新生児 |
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| Research Abstract |
Investigations on the period of postnatal expression of the hyperacute rejection in the discordant xeno-heart transplantation, and explorations of the form of rejections in the post-hyperacute status.
1; Titers of anti-xeno antibodies of the ape gradually increase after birth. Titers 4 month-after birth are significantly lower than those of matured apes, but higher than those of neonates. This fact is considerd to have important relationships with the incidense of the hyperacute rejection after birth.
2; We elected neonatal pigs as donors, and apes 60-79days/21months/24months after birth as recipients. Donor's hearts were extracted after 2-11 days. In the six of FK506 medicated recipients, vascular rejections occurred. In the two of FK506 non-medicated recipients, monocyte infiltrations were revealed. By the TUITEI, stain, TUNEL positive signs were proved in the myocardiac cells.
3; We used same samples for the immunohistologic stain. Egr1 and BTEB2 had already been expressed in 40 minutes after transplantation. In the process of acute vascular rejections, series of substances were sequently expressed, such as EGF, VEGF, MMP2, TIMP2, etc.
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