Analysis of cell death inducing gene in human malignant gliomas
| Project/Area Number |
09470292
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | Kanazawa University |
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Principal Investigator |
YAMASHITA Junkoh Department of Neurosurgery Kanazawa University Chief Proffesor, 医学部, 教授 (90026948)
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| Co-Investigator(Kenkyū-buntansha) |
TACHIBANA Osamu Department of Neurosurgery Kanazawa University Assistant Proffesor, 医学部, 助手 (40211362)
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| Project Period (FY) |
1997 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥6,300,000 (Direct Cost: ¥6,300,000)
Fiscal Year 1998: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1997: ¥4,500,000 (Direct Cost: ¥4,500,000)
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| Keywords | glioma / apoptosis, / Caspase-3 / カスパーゼ3 / Fas / アポトーシス / 神経膠芽腫 / CPP32 / PARP |
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| Research Abstract |
Cysteine proteases of caspase family (interleukin-1 beta -converting enzyme) have been implicated as components of cell death pathway and have been reported to involved in Fas, chemotherapeutic agents, and radiation-induced apoptosis. In this study, I assessed the expression of Caspase-1, Caspase-2 and Caspase-3 in 11 cases of primary astrocytic tumors ( five anaplastic astrocytomas, and six glioblastomas) by reverse transcription (RT)-PCR, Western blot analysis, immunohistochemistry, and in situ casspase-3 activity assay. The frequency of Caspase-1, Caspase-2 and Caspase-3 overexpression appears to correlate with the malignancy grade of astrocytic brain tumors. Furthermore, Caspase-2 and Caspase-3 overexpression and Caspase-3 activation may play an important role in the pathogenesis of necrosis, which is one of the histological hallmarks of glioblastoma.
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Report
(3 results)
Research Products
(9 results)
