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Visualization of calcium signals in acute thromboembolic model of stroke

Research Project

Project/Area Number 09470294
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Cerebral neurosurgery
Research InstitutionMie University

Principal Investigator

KANAMARU Kenji  Mie University School of Medicine, Neurosurgery, Associate Professor, 医学部, 助教授 (70185908)

Co-Investigator(Kenkyū-buntansha) MATSUSHIMA Satoshi  Mie University School of Medicine, Neurosurgery, Assistant Professor, 医学部・附属病院, 講師 (50252367)
久我 純弘  三重大学, 医学部・附属病院, 助手 (70221926)
Project Period (FY) 1997 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥10,600,000 (Direct Cost: ¥10,600,000)
Fiscal Year 1999: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1998: ¥8,000,000 (Direct Cost: ¥8,000,000)
Fiscal Year 1997: ¥1,700,000 (Direct Cost: ¥1,700,000)
Keywordscalcium / cerebral ischemia / rat / thromboembolism / fra-2AM / Rose Bengal / chemiluminescence / ischemic penumbra / カルシウムイオン / 神経細胞死 / 光誘導血栓症モデル / クモ膜下出血 / 平滑筋収縮反応 / ラットモデル / heme oxygenases / endothelin / 脳血栓症
Research Abstract

The increase in intracellular calcium ion plays a pivotal in processing vital function. Both necrosis and apoptosis are triggered by the influx of calcium ion via plasma membrane. The stimuli, such as ischemia and trauma, enhance permeability of calcium ion through plasma membrane. The elevation of intracellular calcium ion activates various proteolytic enzymes and caspases. If the calcium ion level reaches high enough to cause cell dysfunction, either apoptosis or necrosis may be programmed. One can surmise that cell death may be prevented by the inhibition of calcium overload. However, distribution of calcium signals during cerebral ischemia has not yet been demonstrated. We developed a novel method to detect calcium signals in the rat cerebral cortex after thromboembolic ischemia. Exposed rat cerebral cortex was preloaded with fra-2 AM, then Rose Bengal was injected intravenously. The proximal middle cerebral artery was irradiated producing thrombosis with 540 nm wavelength green light. Thereafter, ischemic cerebral cortex was alternatively irradiated with 340 and 380 nm light to emit chemiluminescence. The intensity of chemiluminescence took a peak at 60 minutes after the alternative irradiation. The highest calcium signals occurred in ischemic core which was proved by the pathological examination. In ischemic penumbra calcium signal was moderate. No calcium signal was demonstrated in normal cortex. The region of ischemic penumbra deemed a therapeutic target of rescue from cell death. In conclusion, we demonstrated for the first time calcium signals in the ischemic cerebral cortex in vivo.

Report

(4 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • 1997 Annual Research Report
  • Research Products

    (21 results)

All Other

All Publications (21 results)

  • [Publications] Kanamaru K: "Effect of vasospasm on heme oxygenases…"Stroke. 29. 683-689 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kanamaru K: "Effects of unilateral intrathecal…"Neurol Res . 20. 625-631 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kanamaru K: "Myosin light chain phosphorylation…"Stroke. 29. 2149-2154 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kanamaru K: "Transcranial doppler pattern after…"Neurol Med Chir Suppl (Tokyo). 38. 152-155 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kanamaru K: "Effects of tirilazad mesylate on vasospasm"Stroke. 30. 450-456 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kanamaru K: "Heme oxygenase-1 gene imduction …"J Clin Invest. 104. 59-66 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 金丸憲司: "脳血管2000レビュー"金芳堂. 220 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kuroki M, Kanamaru K, Suzuki H, Waga S, Semba R: "Effect of vasospasm on heme oxygenases in a rat model of subarachnoid hemorrhage"Stroke. 29. 683-689 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Suzuki H, Kanamaru K, Kuroki M, Sun H, Waga S, Miyazawa T: "Effects of unilateral intrathecal administration of low dose of tissue-type plasminogen activator on clot lysis, vasospasm amd brain phospholipid hydroperoxidation in a primate model of bilateral subarachnoid hemorrhage"Neurol Res. 20. 625-631 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Sun H, Kanamaru K, Ito M, Suzuki H, Kojima T, Waga S, Kureishi Y, Nakano T: "Myosin light chain phosphorylation and contractile proteins in a canin two-hemorrhage model of subarachnoid hemorrhage"Stroke. 29. 2149-2154 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kanamaru K, Waga S, Kuga Y, Nakamura F, Kamata N: "Transcranial Doppler pattern after intracarotid papaverine and prostaglandin E_1 incorporated in lipid microsphere in patients with vasospasm"Neurol Med Chir Suppl (Tokyo). 38. 152-155 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Suzuki H, Kanamaru K, Kuroki M, Sun H, Waga S, Miyazawa T: "Effects of tirilazad mesylate on vasospasm and phospholipid Hydroperoxides in a primate model of subarachnoid hemorrhage"Stroke. 30. 450-456 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Suzuki H, Kanamaru K, Tsunoda H, Inada H, Kuroki M, Sun H, Waga S, Tanaka T: "Heme oxygenase-1 gene induction as an intrinsic regulation against delayed cerebral vasospasm in rats"J Clin Invest. 104. 59-66 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kamei Y, Watanabe M, Nakayama T, Kanamaru K, Waga S, Shiraishi T: "Prognostic significance of p53 and p21 WAF1/CIPI immunoreactivity and tumor micronecrosis for recurrence of meningioma"J Neuroconcol. 46. 205-213 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kanamaru K.: "Heme oxygenase-1 gene induction as・・・・"J.Clin.Invest.. 104. 59-66 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Kanamaru K.: "Effects of tirilazad mesylate on・・・・"Stroke. 30. 450-456 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Kanamaru K.: "Effect of vasospasm on heme oxygenases in a rat model of SAH" Stroke. 29. 683-689 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kanamaru K.: "Myosin light chain phosphorylation and contractile proteins..." Stroke. 29. 2149-2154 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kanamaru K.: "Effects of unilateral intrathecal administrations of low dose of..." Neurol.Res.20. 625-631 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kanamaru K.: "Effects of tirilazad mesylate on vasospasm and phospholipid..." Stroke. 30(in press). (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kanamaru K: "Effect of vasospasm on heme oxygenases in a rat model of SAH" Stroke. 29(in press). (1998)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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