| Project/Area Number |
09470307
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | JICHI MEDICAL SCHOOL (1998)
The University of Tokyo (1997) |
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Principal Investigator |
SUGAWARA Yasushi Jichi Medical School, Medical College, Associate Professor, 医学部, 講師 (60260494)
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| Co-Investigator(Kenkyū-buntansha) |
KITANO Yukie Tokyo Univ.Medical College, staff, 医学部, 研究生
SUZUKI Yasutoshi Tokyo Univ.Medical College, staff, 医学部, 研究生
KITAJIMA Akihiko Tokyo Univ.Medical College, staff, 医学部, 助手 (90272541)
梁 淑姫 東京大学, 医学部・附属病院, 医員
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| Project Period (FY) |
1996 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥12,700,000 (Direct Cost: ¥12,700,000)
Fiscal Year 1998: ¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 1997: ¥9,500,000 (Direct Cost: ¥9,500,000)
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| Keywords | osteochondrogenic metabolism / Endo thelin-1 knockout mouse / ET-1 receptors, ETA and ETB / osteonectin / osteopontin / craniofacial dysostosis / Bone elongation / Insituhybridization / endothelin-1 kinockout mouse / Endothelin-1(ET-1) / Endothelin-1 Knockout Mouse / In Situ Hybridization / ET-1 receptors(ETA and ETB) / Osteonectin(ON) / Osteopontin(OP) |
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| Research Abstract |
1) Distribution of endothelin-l receptor (ET-1R) mRNA expression in craniofacial region of mouse fetus at late gestational age has been analyzed by means of in situ hybridization. Of the two ET-1Rs, FT-1RA mRNA was specifically expressed in the osteogenic cells in the craniofacial region, suggesting that these cells are certainly the target cells of FT-1, and that the effects of FT-1 on osteogenic cells are mediated by ET-RA.To clarify how ET-1 modulates differentiation of osteogenic cells, expression of bone matrix protein mRNAs, including osteonectin and osteopontin mRNA, was compared between normal mice and FT-i KG mice that demonstrate severe hypoplasia in facial bones. Neither of osteonectin nor osteopontin mRNA expression was decreased at a cellular level in FT-i KG mice. Therefore we conclude that ET-I may regulate proliferation or migration, rather than differentiation, of osteogenic cells. 2) To induce bone matrix formation in bone defects, osteoprogenitor cells were harvested from the periosteum of a rabbit tibia, expanded in culture and injected into the other tibia or the mandibular bone that was distracted rapidly after osteotome. Bone matrix formation was effectively accelerated by this autologous transplantation of osteoprogenitor cells. This method may be useful for the treatment of large bone gap created by craniofacial surgery.
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