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Role of gas mediators in degradation of articular cartilage

Research Project

Project/Area Number 09470322
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Orthopaedic surgery
Research InstitutionKinki University

Principal Investigator

TANAKA Seisuke  Kinki University School of Medicine, Department of Orthopaedic Surgery, Professor, 医学部, 教授 (00026840)

Co-Investigator(Kenkyū-buntansha) MIYAGI Ichiro  Kinki University, School of Medicine, Department of Orthopaedic Surgery, Instruc, 医学部・附属病院, 助手 (30288906)
OTANI Kazuhiro  Kinki University School of Medicine, Department of Orthopaedic Surgery, Instruct, 医学部, 助手 (20258031)
FUKUDA Kanji  Kinki University School of Medicine, Department of Orthopaedic Surgery, Lecturer, 医学部, 講師 (50201744)
薩摩 博  近畿大学, 医学部・附属病院, 助手 (90288902)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥6,300,000 (Direct Cost: ¥6,300,000)
Fiscal Year 1998: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1997: ¥4,500,000 (Direct Cost: ¥4,500,000)
Keywordschondrocytes / nitric oxide / superoxide / peroxynitrite / proteoglycan / プロデオグリカン
Research Abstract

Although arthrosis, such as rheumatoid arthritis, is one of the popular pathway of joint deterioration, the underlying mechanism of cartilage degradation has not been clarified.
We reported the significance of nitric oxide (NO) and superoxide (SO) in the interleukin-i (IL-1) mediated cartilage degradation. In this series of experiment, we have focused on the mechanism of IL-1-inhibited proteoglycan (PG) synthesis. SIN-1, which leads to simultaneous generation of both NO and su peroxide inhibited PG synthesis. Both superoxide dismutase and hemoglobin reversed SIN-1-inhibited PG synthesis, indicating the simultaneous generation of superoxide is essential to inhibit PG synthesis. IL-1 induced peroxynitrite in articular chondrocytes and addition of peroxynitrite resulted in inhibiting PG synthesis, thereby suggesting that the concurrent generation of superoxide anion and NO is required for the action of IL-1 to inhibit PG synthesis. Peroxynitrite is a candidate for this underlying mechanism.
To examine the role of gas mediator in vivo, transplantation of cartilage was carried out. We made full thickness cartilage defect in the rabbit knee joint (defect model). When perichondrium was transplanted in the defect, degradation of surrounding articular cartilage was inhibited (repair model). The levels of superoxide and NO in the surrounding cartilage of the defect model were significantly higher than the repair model.
These data suggest that gas mediator plays an important role in the cartilage degradation.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (31 results)

All Other

All Publications (31 results)

  • [Publications] M.Oh, et al.: "Concurrent generation of nitric oxide and superoxide inhibits proteglycan synthesis in bovine articular chondrocytes." J Rheumatology. 21. 2169-2174 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] I.Miyagi, et al: "Auto-destruction of the articular cartilage and free radical mediators" J Lab Clin Med. 131. 146-150 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] S.Tanaka, et al.: "Factors related to degeneration of articular cartilage in ostooarthritis・A review" Semin Arthritis Rheum. 27. 392-399 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] M.Ueno, et al.: "Protein kinase C modulates the synthesis of nitric oxide by osteoblasts." Calcif Tissue Int. 63. 22-26 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] S.Tanaka, et al.: "A role for histamine receptors in rheumatoid arthritis." Semin Arthritis Rheum. 26. 824-832 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] K.Fukuda, et al.: "Hyaluronic acid inhibits interleukin-1-induced superoxide anion in bovine chendrocytes" Inflamm Res. 46. 114-117 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] S.Tanaka, et al.: "Advances in osteoarthritis" S.Tanaka, C.Hamanishi, 298 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 田中清介: "慢性関節リウマチ-関節破壊とその対策-" 田中清介, 福田寛二, 177 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] F.Hara: "Pertussis toxin-sensitive G proteins as mediators of stretch-induced decrease in NO release of osteoblast-like cells." J Orthop Res. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] M.Oh: "Concurrent generation of nitric oxide and superoxide inhibits proteoglycan synthesis in bovine articular chondrocytes : involvement of peroxynitrite." J Rheum. 21. 2169-2174 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] M.Ueno: "Protein kinase C modulates the synthesis of nitric oxide by osteoblasts." Calcif Tissue Int. 63. 22-26 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] I Miyagi: "Auto-destruction of the articular cartilage and free radical mediators." J Lab Clin Med. 131. 146-150 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] S Tanaka: "Factros related to degeneration of articular cartilage in osteoarthritis : A review." Rheum. 27. 392-399 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] K Fukuda: "Cyclic tensile stretch on bovine tgchondrocytes inhibits protein kinse C activity" J Lab Clin Med. 130. 209-215 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] S.Tanaka: "A role for histamine receptors in rheumatiod arthritis." Semin Arthritis Rheum. 26. 824-832 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] K Fukuda: "Hyaluronic acid inhibits interleukin-1-induced superoxide anion in bovine chendrocytes." Inflamm Res. 46. 114-117 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] M.Oh,et al.: "Concurrent generation of nitric oxide and superoxide inhibits proteoglycan synthesis in bovine articular chondrocytes." J Rheumatology. 21. 2169-2174 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] I.Miyagi,et al: "Auto-destruction of the articular cartilage and free radical mediators" J Lab Clin Med. 131. 146-150 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] S.Tanaka,et al.: "Factors related to degeneration of articular cartilage in osteoarthritis;A review" Semin Arthritis Rheum. 27. 392-399 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] M.Ueno,et al.: "Protein kinase C modulates the synthesis of nitric oxide by osteoblasts." Calcif Tissue Int. 63. 22-26 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] S.Tanaka,et al.: "A role for histamine receptors in rheumatoid arthritis." Semin Arthritis Rheum. 26. 824-832 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] K.Fukuda,et al.: "Hyaluronic acid inhibits interleukin-1-induced superoxide anion in bovine chendrocytes" Inflamm Res. 46. 114-117 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] S.Tanaka,et al.: "Advances in osteoarthritis" S.Tanaka,C.Hamanishi, 298 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 田中清介: "慢性関節リウマチ-関節破壊とその対策ー" 田中清介,福田寛二, 177 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] S.Tanaka, et al: "Factors related to degradation of articular cartilage in arthritis" Seminar Arth Rheum. (in press).

    • Related Report
      1997 Annual Research Report
  • [Publications] K.Fukuda, et al: "Hyaluronic acid inhibits interleukin-1-induced superoxide anion in bovine chondrocytes" Inflammation Research. 46. 114-117 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] K.Fukuda, et al: "Cyclic tensile stretch loaded on bovine chondrocytes inhibits protein kinaseC activity" J.Laboratory Clinical Medicine. 130. 209-215 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] K.Fukuda, et al: "Hyaluronic acid increases proteoglycan synthesis in bovine articular cartilage in the presence of interleukin-1." J Phar Exp Med. 277. 1672-1675 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] K.Fukuda, et al: "Zonal difference of nitric oxide synthesis with interleukin-1 in bovine chondrocyte" Inflammation Research. 44. 434-437 (1995)

    • Related Report
      1997 Annual Research Report
  • [Publications] K.Fukuda, et al: "Interleukin-1 inhibits keratan sulfate production by rabbit chondrocytes: Possible role of prostaglandin E2." Inflammation Research. 44. 178-181 (1995)

    • Related Report
      1997 Annual Research Report
  • [Publications] 田中清介, 福田寛二: "変形性股関節症" メジカルビュー社, 172 (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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