| Project/Area Number |
09470326
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Anesthesiology/Resuscitation studies
|
|---|
| Research Institution | Mie University |
|---|
Principal Investigator |
MARUYAMA Kazuo Mie University, Faculty of Medicine, professor, 医学部, 教授 (20181828)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
MARUYAMA Junko Mie University, Faculty of Medicine, lecturer, 医学部, 講師 (50263017)
村瀬 澄夫 三重大学, 医学部・附属病院, 助教授 (70200285)
|
|---|
| Project Period (FY) |
1997 – 1998
|
| Project Status |
Completed (Fiscal Year 1998)
|
| Budget Amount *help |
¥9,800,000 (Direct Cost: ¥9,800,000)
Fiscal Year 1998: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1997: ¥7,800,000 (Direct Cost: ¥7,800,000)
|
|---|
| Keywords | nitric oxide / pulmonary hypertension |
|---|
| Research Abstract |
We established the method of isolation of pulmonary vascular smooth muscle cells to investigate the ion channel of hypertensive pulmonary vascular smooth muscle cell. Cultured smooth muscle cells had changes in phenotype and there might be comtamination of other types of cell such as fibroblast. So, we estimated that isolated pulmonary artery vascular smooth muscle was more suitable than cultured cells. Isolation of pulmonary arterial smooth muscle cells was performed in clcium free Tyrode solutioncontaining 20mg collagenase. The cell suspended solution was passed through the nylon mesh. In chronic hypoxia-induced pulmonary hypertensive pulmonary arteries, KCL-induced depolarization-induced-contractions were reduced by NO doner with small changes in intracellular calcium concentration. NO might after calcium sensitivity of pulmonary arterial smooth muscle cells especially in pulmonary hypertension.
|
