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Identification of metastasis suppressor genes for prostate cancer and analysis of genetic changes during progression of prostate cancer.

Research Project

Project/Area Number 09470340
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Urology
Research InstitutionCHIBA UNIVERSITY (1998)
Teikyo University (1997)

Principal Investigator

ICHIKAWA Tomohiko  Chiba University, University Hospital, Lecturer, 医学部・附属病院, 講師 (20241953)

Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥11,100,000 (Direct Cost: ¥11,100,000)
Fiscal Year 1998: ¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 1997: ¥8,000,000 (Direct Cost: ¥8,000,000)
KeywordsProstate cancer / Metastasis / Metastasis suppressor genes / Gene cloning / 分子生物学 / 細胞遺伝学 / 8番染色体 / KAI1遺伝子
Research Abstract

Identification of molecular and cellular markers for the metastatic ability of prostate cancer would be useful in developing diagnostic methods for substaging histologically localized prostate cancers. To isolate metastasis suppressor genes, we have established a rat prostate cancer system. In this system, we used a microcell mediated chromosome transfer technique to introduce a single copy of normal human chromosomes into highly metastatic rat prostate cancer.
In the previous studies, we isolated KAI1/CD82, a metastasis suppressor gene for prostate cancer, from 11p11.2. We also demonstrated that KAI1/CD82 was down- regulated during the progression of human prostate cancer. In the present study, we demonstrate that this down regulation does not require either deletion or point mutation of KAI1/CD 82.
By using the same method, we reported that another metastasis suppressor gene was located on the human chromosome region 8p21-p12. In the present study, this region has been narrowed down to about 1 cM.This is overlapped to the regon where loss of heterozygosity (LOH) has been observed frequently in human prostate cancer We are currently constructing a BAC contig map that covers this 1 cM region.
We have also published that human chromosomes 7 and 12 contain metastasis suppressor genes for prostate cancer. We have also published that human chromosome 19 contains a tumor suppressor gene for prostate cancer.
Human chromosome 13 reduces metastatic ability without suppression of tumorigenicity of rat prostate cancer. We have performed LOU analysis in the human prostate cancer and have shown that the 13q14 is associated with metastasis, which has been published.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (32 results)

All Other

All Publications (32 results)

  • [Publications] Kuramachi, H., et al.: "Suppression of invasive ability of highly metastatic rat prostate cancer by introduction of human chromosome 8." Prostate. 31(1). 14-20 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kuwana, Y., et al.: "Location of KAI1 on the short arm of human chromosome 11 and frequency of alblic loss in advanced human prostate cancer." Prostate. 32(3). 205-213 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Akimoto, S., et al.: "Prostate-specific antigen kvels from a mass screening program using highly sensitive RIA kits." Int.J.Urol.4(3). 269-273 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Akimoto, S., et al.: "Relationship of prostate-specific antigen levels to prostate volume and age in mass screening subjects." Urol.Int.60(4). 216-219 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Luu, H.H., et al.: "Identification of a novel metastasis-suppressor region on human chromosoma 12." Cancer Res.58(16). 3561-3565 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nihei, N., et al.: "Metastasis suppressor gene(s) for rat prostate cancer on the long arm of human chromosome 7." Genes Chromosomes Cancer. 24(1). 1-8 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ueda, T., et al.: "Indentification of a 1-cM region of common deletion on 13q14 associated with metastasis of human prostate cancer." Genes Chromosomes Cancer. 24(2). 183-190 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Gao, A.C., et al.: "Suppression of the tumorigenicity of prostatic cancer cells by gene(s) located on human chromosome 19.p13.1-13.2" Prostate. 38(1). 46-54 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 市川 智彦, 他: "遺伝子異常と腎泌尿器疾患, 前立腺癌" 腎と透析. 42(3). 399-402 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 市川 智彦: "泌尿器科悪性腫瘍-診断と治療・最近の動向, 分子生物学的診断." 腎と透析. 44(6). 743-748 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kuramochi, H., Ichikawa, T., Nihei, N., Kawana, Y., Suzuki, H., Schalken, J.A., Takeichi, M., Nagafuchi, A., and Shimazaki, J.: "Suppression of invasive ability of highly metastatic rat prostate cancer by introduction of human chromosome 8." Prostate. 31(1). 14-20 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kawana, Y., Komiya, A., Ueda, T., Nihei, N., Kuramochi, H., Suzuki, H., Yatani, R., Imai, T., Dong, J-T., Imai, T., Yoshie, O., Barrett, J.C., Isaacs, J.T., Shimazaki, J., Ito.H., and Ichikawa, T.: "Location of KAI1 on the short arm of human chromosome 11 and frequency of allelic loss in advanced human prostate cancer." Prostate. 32(3). 205-213 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Akimoto, S., Ichikawa, T., Akakura, K., and Shimazaki, J.: "Prostate-specific antigen levels from a mass screening program using highly sensitive RIA Kits." Int.J.Urol.4(3). 269-273 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Akimoto, S., Ichikawa, T., and Ito, H.: "Relationship of prostatespecific antigen levels to prostate volume and age in mass screening subjects." Urol.Int.60(4). 216-219 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Luu, H.H., Zagaja, G.P., Dubauskas, Z., Chen, S.L., Smith, R.C., Watabe, K., Ichikawa, T., Davis, E.M., Le Beau, M.M., and Rinker-Schaeffer, C.W.: "Identification of a novel metastasis-suppressor region on human chormosome 12." Cancer Res.58(16). 3561-3565 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nihei, N., Ohta, S., Kuramochi, H., Kugoh, H., Oshimura, M., Barrett, J.C., Isaacs, J.T., Ito, H., Masai, M., Ichikawa, Y., and Ichikawa, T.: "Metastasis suppressor gene(s)for rat prostate cancer on the long arm of human chromosome 7." Genes Chromosomes Cancer. 24(1). 1-8 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Gao, A.C., Lou, W., Ichikawa, T., Denmeade, S.R., Barrett, J.C., and Isaacs, J.T.: "Suppression of the tumorigenicity of prostatic cancer cells by gene(s)located on human chromosome 19p13.1-13.2." Prostate. 38(1). 46-54 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ueda, T., Emi, M., Suzuki, H., Komiya, A., Akakura, K., Ichikawa, T., Shiraishi, T., Masai, M., Igarashi, T., and Ito, H.: "Identification of a 1-cM region of common deletion on 13q14 associated with metastasis of human prostate cancer." Genes Chromosomes Cancer. 24(2). 183-193 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kuramochi,H.,et al: "Suppression of invasive ability of highly metastatic rat prostate cancer by introduction of human chromosome 8." Prostate. 31(1). 14-20 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kawana,Y.,et al.: "Location of KAI1 on the short arm of human chromosome 11 and frequency of allelic loss in advanced human prostate cancer." Prostate. 32(3). 205-213 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] Akimoto,S.,et al.: "Prostate-specific antigen levels from a mass screening program using highly sensitive RIA kits." Int.J.Urol.4(3). 269-273 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] Akimoto,S.,et al.: "Relationship of prostate-specific antigen levels to prostate rolume and age in mass screening subjects." Urol.Int.60(4). 216-219 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Luu,H.H.,et al.: "Identification of a novel metastasis-suppressor region on human chromosome 12." Cancer Res.58(16). 3561-3565 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Nihei,N.,et al.: "Metastasis suppressor gene(s)for rat prostate cancer on the long arm of human chromosome 7." Genes Chromosomes Cancer. 24(1). 1-8 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Ueda,T.,et al.: "Identification of a 1-cM region of common deletion on 13g14 associated with metastasis of human prostate cancer." Genes Chromosomes Cancer. 24(2). 183-190 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Gao,A.C.,et al.: "Suppression of the tumorigenicity of prostatic cancer cells by gene(s)located on human chromosome 19p13,1-13,2." Prostate. 38(1). 46-54 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] 市川智彦,他: "遺伝子異常と腎泌尿器疾患,前立腺癌" 腎と透析. 42(3). 399-402 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] 市川智彦: "泌尿器科悪性腫瘍-診断と治療・最近の動向,分子生物学的診断" 腎と透析. 44(6). 743-748 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kuramochi,Hiroaki: "Suppression of invosive ability of highly metastaric rat prostate cancer by introduction of human chromosome 8." Prostate. 31(1). 14-20 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Kawana,Youko: "Location of KAI1 on the short arm of human chromosome 11 and frequency allelic loss in advanced human prostate cancer." Prostate. 32(3). 205-213 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Gao,Allen C.: "Suppression of the tumorigenicity of prostatic cancer cells by gene(s)lacated on human chromosome 19p13.1-13.2." Int.J.Cancer. (accepted).

    • Related Report
      1997 Annual Research Report
  • [Publications] 市川 智彦: "遺伝子異常と腎泌尿器疾患,前立腺癌" 腎と透析. 42(3). 399-402 (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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