• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Analysis of the genetic background of preterm labor using oxytocin receptor gene.

Research Project

Project/Area Number 09470359
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Obstetrics and gynecology
Research InstitutionOsaka University

Principal Investigator

KANZAKI Toru (1998)  Osaka University Medical School Hospital Lecturer, 医学部・附属病院, 講師 (00263278)

佐治 文隆 (1997)  大阪大学, 医学部, 助教授 (90093418)

Co-Investigator(Kenkyū-buntansha) TOKUGAWA Yoshihiro  Osaka University Medical School, Assistant Professor, 医学部, 助手 (70283786)
MITSUDA Nobuaki  Osaka University Medical School, Assistant Professor, 医学部, 助手 (50209805)
OHASHI Kazutomo  Osaka University Medical School, Lecturer, 医学部, 講師 (30203897)
AZUMA Chihiro  Osaka University Medical School, Lecturer, 医学部, 講師 (20151061)
MURATA Yuji  Osaka University Medical School, Professor, 医学部・附属病院, 教授 (40283759)
神崎 徹  大阪大学, 医学部, 助手
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥10,700,000 (Direct Cost: ¥10,700,000)
Fiscal Year 1998: ¥6,400,000 (Direct Cost: ¥6,400,000)
Fiscal Year 1997: ¥4,300,000 (Direct Cost: ¥4,300,000)
KeywordsOxytocin receptor / N-gycosylation pont mutation / Mammary gland / DNA binding protein / U-Maf / U-Maf / 早産 / 早期発現 / 遺伝的背景 / 突然変異体 / 脱感作現象
Research Abstract

1. Effect of glycosylation on the ligand binding of oxytocin receptor
In order to determine "hot point" of the oxytocin receptor function, we introduced point mutations in the N-glycosylation site of human oxytocin receptor cDNA.The matant (deglycosylated) oxytocin receptors were introduced into Hela cells. The binding profile of the mutant OTR to various oxytocin related ligands was identical to the wild type OTR, indicating that the N-glycosylation of OTR molecule is not necessary for the receptor function.
2. OTR expression in the human mammary gland.
Milk ejection is important function for nursing mature and premature infant. We investigated the localization of OTR in human non-lactating and lactating mammary gland and indicated that OTR is mainly localized in ductal/glandular epithelium. The expression level of OTR mORNA and protein was low, even during the lactating period.
3. Molecular cloning of the DNA-binding protein to 5'-region of human OTR gene.
We have cloned one DNA-binding protein (U-Mat) which bind specifically to the human OTR gene. U-Maf is from 164 aminoacids and the molecular wight is 18KD.The function of U-Maf at parturition or preterm labor is not known. It is necessary to elucidate the function of this molecular during the process of uterine activation.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (17 results)

All Other

All Publications (17 results)

  • [Publications] Kimura,T.at al: "The role of N-terminal glycosylation in human oxytocin receptor" Mol.Hum.Reprod.11. 957-963 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Phaneuf,S.et al: "The desensitization of oxytocin receptors in human myometrial cells is accompanished by down-regulation of oxytocin receptor messenger RNA" J.Endocrinol.154. 7-18 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kimura,T.et al: "Expression and immunolocalization of the oxytocin receptor in human lactating and non-lactating mammary glands" Hum.Reprod.13. 2645-2653 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Miyai,K.et al: "Effect of directed mutagenesis in the CMGCC region of the α-subunit on immunoreactive human thyrotropin" Endocrine J.45. 467-473 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tokugawa,Y.et al: "Lipocalin-type prostaglandin D synthase in human reproductive organs and seminal plasma" Biol.Reprod.58. 600-607 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kimura, T.et al: "The role of N-terminal glycosylation in human oxytocin receptor" Mol.Hum.Reprod.11. 957-963 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Phaneuf, S.et al: "The desensitization of oxytocin receptors in human myometrial cells is accompanished by down-regulation of oxytocin receptor messenger RNA" J.Endocrinol.154. 7-18 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kimura, T.et al: "Expression and immunolocalization of the oxytocin receptor in human lactating and non-lactating mammary glands" Hum.Reprod.13. 2645-2653 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Miyai, K.et al: "Effect of directed mutagenesis in the CMGCC region of the alpha-subunit on immunoreactive human thyrotropin" Endocrine J.45. 467-473 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tokugawa, Y.et al: "Lipocalin-type prostaglandin D synthase in human reproductive organs and seminal plasma" Biol.Reprod.58. 600-607 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kimura,T. et al: "The role of N-terminal glycosylation in human oxytocin receptor" Mol.Hum.Reprod.11. 957-963 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] Phaneuf,S. et al: "The desensitization of oxytocin receptors in human myometrial cells is accompanished by down-regulation of oxytocin receptor messenger RNA" J.Endocrinol.154. 7-18 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kimura,T. et al: "Expression and immunolocalization of the oxytocin receptor in human lactating and non-lactating mammary glands" Hum.Reprod.13. 2645-2653 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Miyai,K. et al: "Effect of directed mutagenesis in the CMGCC region of the α-subunit on immunoreactive human thyrotropin" Endocrine J.45. 467-473 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Tokugawa,Y. et al: "Lipocalin-type prostaglandin D systhase in human reproductive organs and seminal plasma" Biol.Reprod.58. 600-607 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] T.Kimura, Y.Makino, F.Saji, Y.Murata, et al.: "The role of N-terminal glycosylation in the human oxytocin receptor." Molecular Human Reproduction. 3(11). 957-963 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] S.Phaneuf, F.Saji, T.Kimura, et al.: "The desensitization of oxytocin receptors in human myometnal myometnal cell is accompanied by down regulation of oxytocin receptor mRNA." Journal of Endocrinology. 154. 7-18 (1997)

    • Related Report
      1997 Annual Research Report

URL: 

Published: 1997-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi