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Molecular mechanism of endometrial cancer and its application to gene diagnosis

Research Project

Project/Area Number 09470362
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Obstetrics and gynecology
Research InstitutionKYUSHU UNIVERSITY

Principal Investigator

WAKE Norio  Medical Institute of Bioregulation, Kyushu Univ., Professor, 生体防御医学研究所, 教授 (50158606)

Co-Investigator(Kenkyū-buntansha) NISHIDA Jun-ichi  Medical Institute of Bioregulation, Kyushu Univ., Research Associate, 生体防御医学研究所, 助手 (40264113)
KATO Kiyoko  Medical Institute of Bioregulation, Kyushu Univ., Assistant Professor, 生体防御医学研究所, 講師 (10253527)
KATO Hidenori  Medical Institute of Bioregulation, Kyushu Univ., Assistant Professor, 生体防御医学研究所, 講師 (60214392)
MATSUDA Takao  Medical Institute of Bioregulation, Kyushu Univ., Research Associate, 生体防御医学研究所, 助手 (10304825)
有馬 隆博  九州大学, 生体防御医学研究所, 助手 (80253532)
Project Period (FY) 1997 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥15,400,000 (Direct Cost: ¥15,400,000)
Fiscal Year 1999: ¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1998: ¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 1997: ¥9,500,000 (Direct Cost: ¥9,500,000)
Keywordsendometrial cancer / a tumor suppressor gene on 1q / cell senescence / Ras / ER / DPC4 / DOC / cyclin G / tumor supppressor gene on 1q / 癌抑制遺伝子 / 子宮体癌 / 1番染色体 / 微小核融合 / 変異型K-Ras / エストロゲンリセプター / 形質転換 / 変異型K・Ras
Research Abstract

1. Identification of an endometrial cancer suppressor gene locus : The gene located chromosome 1q has an ability to induce senescence in endometrial cancer cells. By introducing a del 1q chromosome that has incurred an interstitial deletion of approximately 63 cM or subchromosomal transferable fragments derived from the 1q into the endometrial cancer cells, we identified the localization of a target gene within 1q31-ter. In addition, we analyzed LOH on 1q using 60 sample of endometrial cancers and narrowed down the localization with 1q42.
2. Contribution of ER to Ras mediated transformation. Mutant K-ras protein had an ability to induce expression of ER protein (3-fold) that functioned as a transcription factor. Reconstituted cells that had enhanced ER activities were tumorigenic but the cells with lower ER activities were nontumorigenic. Suppression of ER activities resulted in the loss of tumorigenicity in the cells transformed by mutant k-ras.
3. Both DPC4 and DOC genes targeted by 18q LOH in endometrial cancers. : 18qLOH is frequent in endometrial cancers. By making a detailed deletions map on 18q, we found that deletions targeted both DPC4 and DOC genes. Mutations of the DPC4 promoter regions in the remaining allele resulted in the suppression of DPC4 gene activities. In turn, transfection of DOC cDNA resulted in the suppression of tumorigenicity or the induction of apoptosis in endometrial cancer cells. A DOC gene functions as a tumor suppressor gene by inducing apoptosis of endometrial cells.
4. Function of p53-dependent cyclin G : Dox treatment induced p53 accumulation, p21 and cyclin G transcription, that resulted in G2/M arrest of cells. In turn, NaB induced p53 accumulation and p21 transcription but not cyclin G transcription, that resulted in G1 arrest of cells. Suppression of cyclin G expression corresponded to the release of cells from G2/M. Overexpression of cyclin G reverted the cell susceptible to apoptosis.

Report

(4 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • 1997 Annual Research Report
  • Research Products

    (30 results)

All Other

All Publications (30 results)

  • [Publications] Kato H et al: "Suppressed tumoigenicity of human endometrial cancer cells by the restored expression of DCC gene"Br. J. Cancer. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Zhou Y et al: "involvement of mutations in the DPC4 promoter in endometrial carcinoma development"Molecular Carcinogenesis. 25. 64-72 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kato K et al: "Oncogenic Ras Modulates epidermal groth factor responsiveness in endometiral carinomas."European J. Cancer. 34,5. 737-744 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Shimizu A et al: "CyclinG Contributes to G2/M arrest of cells in response to DNA Damage."Biochemical and Biophysical Research Communications. 242. 529-533 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kato K et al: "Contribution of enhanced transcriptional activation by ER to [12 val] K-Ras mediated NIH3t3 cell transformation."Oncogene. 15. 3037-3046 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Matsuda T et al: "Human chromosome 7 carries a putative tumor suppressor gene(s) involved in choriocarcinoma"Oncogene. 15. 2773-2781 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Wake N et al: "New insights in Gynecology and Obstetrics"Genetics of gynecological cancer: molecular events implicated in trophoblastic neoplasia development. 7 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kato H. et al: "Suppressed tumorigenicity of human endometrial cancer cells by the restored expression of DOC gene"Br. J. Cancer. (inpress).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Zhou Y. et al: "Involvement of mutations in the DPC4 promoter in endometrial carcinoma development"Molecular Carcinogenesis. 25. 64-72 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kato K. et al: "Oncogenic Ras modulates epidermal growth factor responsiveness in endometrial carcinomas"European J. Cancer. 34, 5. 737-744 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Shimuzu A. et al: "Cyclin G Contributes to G2/M arrest of cells in response to DNA Damage"Biochemical and Biophysical Research Communications. 242. 529-533 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Wake N. et al: "Genetics of gynecological cancer : molecular events implicated in trophoblastic neoplasia development"New insights in Gynecology and Obstetrics. 38-45 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kato K. et al: "Contribution of enhanced transcriptional activation by RE to [ィイD112ィエD1val] K-Ras mediated NIH3T3 cell transformation"Oncogene. 15. 3037-3046 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Matsuda T. et al: "Human chromosome 7 carries a putative tumor suppressor gene(s) involved in choriocarcinoma"Oncogene. 15. 2773-2781 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Murakami A et al: "Photodynamic antisense regulation (PDAR) of human cervical carcinoma cell growth using psoralen-conjugated oligo (nucleoside phosphorothioate)."European Journal of Pharmaceutical Science. in press.

    • Related Report
      1999 Annual Research Report
  • [Publications] Ueoka Y et al: "HGF and Ovarian carcinoma cell motility and invasion :"British Journal of Cancer. in press.

    • Related Report
      1999 Annual Research Report
  • [Publications] Hachiya T et al: "WAF1 Genotype and Endometrial Cancer Susceptibility"Gynecologic Oncology. 72. 187-192 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Zhou Y et al: "Involvement of mutations in the DPC4 promoter in endometrila carcinoma development."Molecular Carcinogenesis. 25. 64-72 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Kato K et al: "Relevance of ER to the development of endometrial hyperplasia and adenocarcinoma."Breast Cancer. 6. 312-319 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Shimizu.A.et al: "CyclinG Contributes to G2/M arrest of cells inresponse to DNA Damage" Biochemical and Biophysical Research Communications. 242. 529-533 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kato.K.,et al: "Oncogenic Ras modulates epidermal growth factor responsive ness in end ometiral carcinomas" European J.Cancer. 34・5. 737-744 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kato.K.,et al: "Requirement of Estrogen Receptor Expression and Function for [12Val] K‐RasMediated NIH3T3 Cell Transformation" Oncoiogy. 55. 45-52 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Sakamoto.T.,et al: "Microsatellite Instability and Endometrial Carcinomas" Gynecol Oncoiogy. 71. 53-58 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Hachiya.T.,et al: "WAF1 Genotype and Endometrial Cancer Susceptibility" Gynecologic Oncology. in press.

    • Related Report
      1998 Annual Research Report
  • [Publications] Zhou.Y.,et al: "lnvolvement of mutations in the DPC4 promoter in endomet rial carcino ma development" Molecular Carcinogenesis. in press.

    • Related Report
      1998 Annual Research Report
  • [Publications] Kanuma T,et al: "Alterations of the p16^<IKK4A> gene in human ovarian cancers." Molecular Carcinogenesis. 18. 134-141 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Kato K,et al: "Analysis of danazol action:Endometriosis today." Parthenon Publishing. 381-385 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Kato K,et al: "Contribution of enhanced transcriptional activation by ER to 〔^<12>val〕 K-Ras mediated NIH3T3 cell transformation." Oncogene. 15. 3037-3046 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Kato K,et al: "Oncogenic Ras modulates epidermal growth factor responsiveness in endometiral carcinomas." The European Journal of Cancer. (in press).

    • Related Report
      1997 Annual Research Report
  • [Publications] Shimizu A,et al: "CyclinG Contributes to G2/M arrest of cells in response to DNA Damage." Biochemical and Biophysical Research Communications. (in press).

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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