| Project/Area Number |
09470367
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Otorhinolaryngology
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| Research Institution | Tohoku University |
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Principal Investigator |
IKEDA Katsuhisa Medical School, Tohoku University Assistant Professor, 医学部, 講師 (70159614)
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| Co-Investigator(Kenkyū-buntansha) |
OSHIMA Takeshi Medical School, Tohoku University Lecturer, 医学部, 助手 (40241608)
志賀 伸之 東北大学, 医学部・附属病院, 助手 (10215957)
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| Project Period (FY) |
1997 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥11,900,000 (Direct Cost: ¥11,900,000)
Fiscal Year 1998: ¥4,900,000 (Direct Cost: ¥4,900,000)
Fiscal Year 1997: ¥7,000,000 (Direct Cost: ¥7,000,000)
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| Keywords | Channel / Transporter / Cochlea / Hearing / In-situ hybridization / RT-PCR / mRNA / Strial Vascularis / 血管系 / 輸送体蛋白 / PCR / DNA / in situ hybridization / イオン輸送 / クローニング |
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| Research Abstract |
A variety of ionic channels and transportes are known to be related to inner ear function. We have so far found Cl voltage-dependent chloride channels (ClC), Na-K-2C1 cotransporter (CCC), and Na-Ca exchanger (NCX), and Na-H exchange (NHE) in the cochlea by physiological methods. Furthermore, recent advances in molecular biology enabled us to prove the definite evidence of molecular characterization of these ion chanells and transportes. We used the reverse transcription-polymerase chain reaction (RT-PCR) to assess the expression of various isoforms and the non-radioactive in situ hybridization (ISH) to examine their localization. Among CIC family, ClC-2 and -3 were significantly present in the inner ear tissue. BSC2 transcript alone was detected in the cochlea and intensively located in the strial marginal cells and spiral ligament fibrocytes, indicating a good explanation of loop-diuretics cochleotoxicity. All of three isoforms of NCX (1-3) were detected in the inner ear. Unique spicing varients of NCX-1 were found in the cochlear modiolus. Although NHE-2, -3, and -4 isoform mRNAs could be detected in the cochlear tissue, NHE-1 message was clearly dominant. NHE-1 isoform expression was distributed in the hair cells, marginal cells, and spiral ligament fibrocytes, and ganglion cells.
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