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Expression and significance of MIF in the ocular tissue.

Research Project

Project/Area Number 09470376
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Ophthalmology
Research InstitutionHOKKAIDO UNIVERSITY

Principal Investigator

TAGAWA Yoshitsugu  Hokkaido Univ., Sch. Of Med., Associate Professor, 医学部, 助教授 (40109426)

Co-Investigator(Kenkyū-buntansha) MATSUDA Akira  Hokkaido Univ., Sch. Of Med., Instrsuctor, 医学部, 助手 (00312348)
OGASAWARA Kazumasa  Hokkaido Univ., Institute of Immunological Science, Associate Professor, 免疫科学研究所, 助教授 (20169163)
NISHIHIRA Jun  Hokkaido Univ., Sch. Of Med., Associate Professor, 医学部, 助教授 (30189302)
Project Period (FY) 1997 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥12,600,000 (Direct Cost: ¥12,600,000)
Fiscal Year 1999: ¥4,200,000 (Direct Cost: ¥4,200,000)
Fiscal Year 1998: ¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 1997: ¥4,800,000 (Direct Cost: ¥4,800,000)
Keywordsmacrophage migration inhibitory factor / embryonic eye / normal eye / corneal perforative wound / experimental autoimmune uveoretinitis / proliferative vitreoretinopathy / 実験的自己免疫性ぶとう膜炎 / 角膜穿孔剤
Research Abstract

In order to clarify the expression and significance of macrophage migration inhibitory factor (MIF) in the ocular tissue, we examined the following subjects; 1)the expression of MIF on embryonic and normal eye, 2)the role of MIF in corneal wound healing, 3)the role of MIF in experimental autoimmune uveoretinitis(EAU), 4)the significance of MIF in proliferative vitreoretinopathy(PVR). The results showed 1) MIFmRNA reached in the highest level in E1 3 days and MIF distributed much in lens epithelium and retinal pigment epithelium(RPE). In normal eye MIF expressed in cornral epithelium and endothelium, lens epithelium, iris-ciliary body epithelium, and retinal glial cells and RPE in retina, 2) MIF disappeared in cornea 3 hrs after corneal perforation wound and reappeared in 6 hrs. MIF increased in aquaous humor (AH) 6 hrs after perforation(119.6ng/ml), 3) MIF increased in AH and cerebrospinal fluid in EAU and reached the heighest levels in Day 13 (442ng/ml, 203ng/ml), and anti MIF antibodies delayed the onset of EAU and decreased the inflammation of EAU, 4) the vitreous samples of PVR revealed the increased levels of MIF compared with those of retinal detachment and macular hole. The above findings indicate that MIF play a role in the eye development, wound healing, inflammation and the pathogenesis in several vitreoretinal diseases in the ocular tissue.

Report

(4 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • 1997 Annual Research Report
  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] Matsuda A.: "Expression of macrophage migration inhibitory factor in rat retina and its immunohistochemical localization"Journal of Neuroimmunology. 77・1. 85-90 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Matsuda A.: "Expression of macrophage migration inhibitory factor in corneal wound healiy in rats"Investigative Oplubalmology & Visual Science. 38・8. 1555-1562 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Mitamura Y.: "Macrophage migration inhibitory factor levels in the vitreous of patients with proliferative vitreoretinopathy"American Journal of Ophthalmology. 128・6. 763-765 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kitaichi N.: "Inhibition of exporimental autoimmune uveoretinitis with anti-macrophage migration inhibitory factor antibodies"Current Eye Research. 20・2. 109-114 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Tagawa Y.: "Current Opinion in the Kyoto Cornea Club, vol.3 「Cornea and macrophage migration inhibitory factor"Kugler Publications, The Hague. 125(71-77) (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Matsuda A, Tagawa Y, Yoshida K, Matsuda H, Nishihira J.: "Expression of macrophage migration inhibitory factor in rat retina and its immunohistochemical localization."J Neuroimmunol. 77. 85-90 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Matsuda A, Tagawa Y, Matsuda H, Nishihira J.: "Expression of macrophage migration inhibitory factor in corneal wound healing in rats."Invest Ophthalmol Vis Sci.. 38. 1555-1562 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Mitamura Y, Takeuchi S, Matsuda A, Tagawa Y, Mizue Y, Nishihira J.: "Macrophage migration inhibitory factor levels in the vitreous of patients with proliferative vitreoretinopathy."Am J Ophthalmol. 128. 763-765 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kitaichi N, Matsuda A, Kotake S, Namba K, Tagawa Y, Sasamoto Y, Ogasawara K, Iwabuchi K, Onoe K, Matsuda H, Nishihira J.: "Inhibition of experimental autoimmune uveoretinitis with anti-macrophage migration inhibitory factor antibodies."Curr Eye Res. 20. 109-114 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Tagawa Y.: "Cornea and crophage migration inhibitory factor."(Kinoshita S, Ohashi Y. eds. Current Opinion in the Kyoto Cornea Club, vol.3, Kugler Publications, The Hague). 71-77 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Mitamura, Y., et al.: "Macrophage migration inhibitory factor levels in the vitreous of patients with proliferative vitreoretinopathy"Am. J. Ophthalmol.. 128・6. 763-765 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Kitaichi, N., et al.: "Inhibition of experimental autoimmune ureoretinitis with anti-macrophage migration inhibitory factor antibodies"Curr. Eye Res.. 20・2. 109-114 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Matsuda, Tagawa,Y: "Expression of macrophage migration inhilitory,factor in rat retina and ito immcnobilitochamical locali fation" Journal of Neuroimmunology. 77・1. 85-90 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Matsuda, Tagawa,Y: "Expression of macrophage migration inhilutory factor in cormeal wound kealing in rato" Investigatine Ophthalmology & Visual Science. 38・3. 1555-1562 (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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