| Project/Area Number |
09470376
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Ophthalmology
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| Research Institution | HOKKAIDO UNIVERSITY |
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Principal Investigator |
TAGAWA Yoshitsugu Hokkaido Univ., Sch. Of Med., Associate Professor, 医学部, 助教授 (40109426)
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| Co-Investigator(Kenkyū-buntansha) |
MATSUDA Akira Hokkaido Univ., Sch. Of Med., Instrsuctor, 医学部, 助手 (00312348)
OGASAWARA Kazumasa Hokkaido Univ., Institute of Immunological Science, Associate Professor, 免疫科学研究所, 助教授 (20169163)
NISHIHIRA Jun Hokkaido Univ., Sch. Of Med., Associate Professor, 医学部, 助教授 (30189302)
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| Project Period (FY) |
1997 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥12,600,000 (Direct Cost: ¥12,600,000)
Fiscal Year 1999: ¥4,200,000 (Direct Cost: ¥4,200,000)
Fiscal Year 1998: ¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 1997: ¥4,800,000 (Direct Cost: ¥4,800,000)
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| Keywords | macrophage migration inhibitory factor / embryonic eye / normal eye / corneal perforative wound / experimental autoimmune uveoretinitis / proliferative vitreoretinopathy / 実験的自己免疫性ぶとう膜炎 / 角膜穿孔剤 |
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| Research Abstract |
In order to clarify the expression and significance of macrophage migration inhibitory factor (MIF) in the ocular tissue, we examined the following subjects; 1)the expression of MIF on embryonic and normal eye, 2)the role of MIF in corneal wound healing, 3)the role of MIF in experimental autoimmune uveoretinitis(EAU), 4)the significance of MIF in proliferative vitreoretinopathy(PVR). The results showed 1) MIFmRNA reached in the highest level in E1 3 days and MIF distributed much in lens epithelium and retinal pigment epithelium(RPE). In normal eye MIF expressed in cornral epithelium and endothelium, lens epithelium, iris-ciliary body epithelium, and retinal glial cells and RPE in retina, 2) MIF disappeared in cornea 3 hrs after corneal perforation wound and reappeared in 6 hrs. MIF increased in aquaous humor (AH) 6 hrs after perforation(119.6ng/ml), 3) MIF increased in AH and cerebrospinal fluid in EAU and reached the heighest levels in Day 13 (442ng/ml, 203ng/ml), and anti MIF antibodies delayed the onset of EAU and decreased the inflammation of EAU, 4) the vitreous samples of PVR revealed the increased levels of MIF compared with those of retinal detachment and macular hole. The above findings indicate that MIF play a role in the eye development, wound healing, inflammation and the pathogenesis in several vitreoretinal diseases in the ocular tissue.
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