| Project/Area Number |
09470380
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Ophthalmology
|
|---|
| Research Institution | Okayama University |
|---|
Principal Investigator |
MATSUO Toshihiko Okayama University Medical School Hospital, Lecturer, 医学部・附属病院, 講師 (90211565)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
NAKAGAWA Hideki Okayama University Medical School Hospital, Assistant, 医学部・附属病院, 助手 (10252967)
中ノ森 恒 岡山大学, 医学部, 助手 (00252966)
|
|---|
| Project Period (FY) |
1997 – 1999
|
| Project Status |
Completed (Fiscal Year 1999)
|
| Budget Amount *help |
¥10,300,000 (Direct Cost: ¥10,300,000)
Fiscal Year 1999: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1998: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1997: ¥7,200,000 (Direct Cost: ¥7,200,000)
|
|---|
| Keywords | trabecular cells / cyclic mechanical stretching / TIMP-1 / TIMP-2 / ひき算ハイブリダイゼーション / クローン / MMP-2 / 塩基配列 |
|---|
| Research Abstract |
We demonstrated that cultured bovine trabecular cells produced tissue inhibitor of metalloproteinase-1 (TIMP-1) and matrix metalloproteinase-2 (MMP-2) in response to cyclic mechanical stretching for 72 hours. Cyclic mechanical stretching was applied to cultured cells in 6-cm petri dishes at the magnitude of 4500 microstrain and at a cycle of 30 seconds. Human trabecular cells also expressed a novel gene in response to cyclic mechanical stretching for 24 hours. This new gene was isolated by subtractive hybridization based on suppression polymerase chain reaction. Messenger RNA was isolated from stretched trabecular cells and control trabecular cells, and cDNA was synthesized. Control cell-derived cDNA was subtracted from stretched cell-derived cDNA. The subtracted cDNA fragments were cloned into a plasmid vector. The expression of each clone in stretched cells and in control cells was examined by Northern blot hybridization. The corresponding full-length cDNA was then isolated by screening of human eye cDNA LIBRARY. These facts suggest that trabeuclar cells respond to mechanical stimuli and that an intraocular-sensing mechanism is present in trabecular cells.
|
