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Molecular biological and biochemical study of intraocular pressure-sensing mechanism

Research Project

Project/Area Number 09470380
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Ophthalmology
Research InstitutionOkayama University

Principal Investigator

MATSUO Toshihiko  Okayama University Medical School Hospital, Lecturer, 医学部・附属病院, 講師 (90211565)

Co-Investigator(Kenkyū-buntansha) NAKAGAWA Hideki  Okayama University Medical School Hospital, Assistant, 医学部・附属病院, 助手 (10252967)
中ノ森 恒  岡山大学, 医学部, 助手 (00252966)
Project Period (FY) 1997 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥10,300,000 (Direct Cost: ¥10,300,000)
Fiscal Year 1999: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1998: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1997: ¥7,200,000 (Direct Cost: ¥7,200,000)
Keywordstrabecular cells / cyclic mechanical stretching / TIMP-1 / TIMP-2 / ひき算ハイブリダイゼーション / クローン / MMP-2 / 塩基配列
Research Abstract

We demonstrated that cultured bovine trabecular cells produced tissue inhibitor of metalloproteinase-1 (TIMP-1) and matrix metalloproteinase-2 (MMP-2) in response to cyclic mechanical stretching for 72 hours. Cyclic mechanical stretching was applied to cultured cells in 6-cm petri dishes at the magnitude of 4500 microstrain and at a cycle of 30 seconds. Human trabecular cells also expressed a novel gene in response to cyclic mechanical stretching for 24 hours. This new gene was isolated by subtractive hybridization based on suppression polymerase chain reaction. Messenger RNA was isolated from stretched trabecular cells and control trabecular cells, and cDNA was synthesized. Control cell-derived cDNA was subtracted from stretched cell-derived cDNA. The subtracted cDNA fragments were cloned into a plasmid vector. The expression of each clone in stretched cells and in control cells was examined by Northern blot hybridization. The corresponding full-length cDNA was then isolated by screening of human eye cDNA LIBRARY. These facts suggest that trabeuclar cells respond to mechanical stimuli and that an intraocular-sensing mechanism is present in trabecular cells.

Report

(4 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • 1997 Annual Research Report
  • Research Products

    (17 results)

All Other

All Publications (17 results)

  • [Publications] Yumiko Okada, Toshihiko Matsuo, Hiroshi Ohtsuki: "Bovine trabecular cells produce TIMP-1 and MMP-2 in response to mechanical stretching"Japanese Journal of Ophthalmology. 42. 90-94 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Toshihiko Matsuo: "In situ visualization of messenger RNA for basic blast growth factor in living cells"Biochimica et Biophysica Acta. 1379. 178-184 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Toshihiko Matsuo, Yumiko Okada, Fumio Shiraga, Toshihiro Yanagawa: "TIMP-1 and TIMP-2 levels in vitreous and subretinal fluid"Japanese Journal of Ophthalmology. 42. 377-380 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Toshihiko Matsuo: "Expression of amiloride-sensitive channel in rat eye"Acta Medica Okayama. 52. 279-283 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yukiko Sato, Toshihiko Matsuo, Hiroshi Ohtsuki: "A novel gene (oculomedin) induced by mechanical stretching in human trabecular cells of the eye"Biochemical & Biophysical Research Communications. 259. 349-351 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yumiko Okada, Toshihiko Matsuo, Hiroshi Ohtsuki.: "Bovine trabecular cells produce TIMP-1 and MMP-2 in response to mechanical stretching"Japanese Journal of Ophthalmology. 42. 90-94 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Toshihiko Matsuo.: "In situ visualization of messenger RNA for basic fibroblast growth factor in living cells"Biochemica et Biophysica Acta. 1379. 178-184 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Toshihiko Matsuo, Yumiko Okada, Fumio Shiraga, Toshihoro Yanagawa: "TIMP-1 and TIMP-2 levels in vitreous and subretinal fluid"Japanese Journal of Ophthalmology. 42. 377-380 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Toshihiko Matsuo.: "Expression of amiloride-sensitive channel in rat eye"Acta Medica Okayama. 52. 279-283 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yukiko Sato, Toshihiko Matsuo, Hiroshi Ohtsuki.: "A novel gene (oculomedin) induced by mechanical stretching in human trabecular cells of the eye"Biochemical and Biophysical Research Communications. 259. 349-351 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yukiko Sato,Toshihiko Matsuo,Hiroshi Ohtsuki: "A novel gene (oculomedin) induced by mechanical stretching in human trabecular cells of the eye"Biochemical & Biophysical Research Communications. 259. 349-351 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Yumiko Okada,Toshihiko Matsuo: "Bovine trabecular cells produce TIMP-1 and MMP-2 in response to Mechanical stretching" Japanese Jouenal of Ophthalmology. 42. 90-94 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Toshihiko Matsuo: "In situ visualization of messenger RNA for basic fibroblast growth factor in living cells" Biochimica et Biophysica Acta. 1379. 178-184 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Toshihiko Matsuo: "TIMP-1 and TIMP-2 levels in vitreous and subretinal fluid" Japanese Journal of Ophthalmology. 42. 377-380 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Toshihiko Matsuo: "Expression of amiloride-sensitive channel in rateye" Acta Medica Okayama. 52. 279-283 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Yumiko Okada, Toshihiko Matsuo, Hiroshi Ohtsuki: "Bovine trabecular cells produce TIMP-1 and MMP-2 in response to mechanical stretching" Japanese Journal of Ophthalmology. 42(in press). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Toshihiko Matsuo: "In situ visualization of messenger RNA for basic fibroblast growth factor to living cells" Biochimica et Biophysica Acta. (in press). (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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