| Project/Area Number |
09470385
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| Research Category |
Grant-in-Aid for Scientific Research (B).
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Ophthalmology
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| Research Institution | Kansai Medical University |
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Principal Investigator |
UYAMA Masanobu Kansai Medical University, Department of Ophthalmology, Professor and Chairman, 医学部, 教授 (30025580)
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| Co-Investigator(Kenkyū-buntansha) |
MATSUSHIMA Masashi Kansai Medical University, Assistant Professor, 医学部, 講師 (00192336)
TAKAHASHI Kanji Kansai Medical University, Assistant Professor, 医学部, 講師 (60216710)
OGATA Nahoko Kansai Medical University, Assistant Professor, 医学部, 講師 (60204062)
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| Project Period (FY) |
1997 – 1998
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥13,200,000 (Direct Cost: ¥13,200,000)
Fiscal Year 1998: ¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 1997: ¥9,600,000 (Direct Cost: ¥9,600,000)
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| Keywords | age-related macular degeneration / choroidal neovascularization / retinal pigment epithelium / angiogenic factor / basic fibroblast growth factor / vascular endothelial growth factor / gene therapy / gene induction / 網膜虚血再潅流 / VEGF / b-FGF / インターフェロンβ / 細胞成長因子 |
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| Research Abstract |
1. Molecular modulation in choroidal neovascularization
Choroidal neovascularization (CNV) was induced in monkey and rat eyes by intense laser photocoagulations of the retina in the posterior pole. In experimental CNV, expression of mRNA of b-fibroblast growth factor (FGF), vascular endothelial growth factor (VEGF), trans-forming growth factor (TGF)-β, and their receptors were revealed, using in situ hybridization and immunohistochemistry, markedly in the early stage in developing of CNV, and slightly in the late stage. These results suggest development and regression of CNV were modulated by these cytokines.
2. Effect of Interferron β on development of CNV
Local (subtenon) or systemic administration of Interferron (IFN)-β inhibited development of CNV.Involution of CNV were due to enclosure by proliferatin of the retinal pigment epithelium (RPE). IFN-β upregulated expression of angiogenic
factors such as bFGF and VEGF in CNV.
3. Possibility of gene therapy in CNV
A vector of combination of hemagglutinating Virus of Japan (HVJ) and liposome was enveloped with antisense-olignucleotide or antisense plasmid, then intoroduced to CNV or RPE in vivo. They were successfully transferred in the target cells.
These results show gene therapy with HVJ-liposome method will be achieved effectively in CNV treatment.
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