Deletion mapping of chromosome band 14q32 for isolation and characterization of the putative tumor related gene in human neuroblastoma
| Project/Area Number |
09470386
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
小児外科
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| Research Institution | Tohoku University |
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Principal Investigator |
HAYASHI Yutaka Tohoku University School of Medicine Professor, 医学部, 教授 (40125638)
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| Co-Investigator(Kenkyū-buntansha) |
HORII Akira Tohoku University School of Medicine Professor, 医学部, 教授 (40249983)
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| Project Period (FY) |
1997 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥13,100,000 (Direct Cost: ¥13,100,000)
Fiscal Year 1998: ¥4,400,000 (Direct Cost: ¥4,400,000)
Fiscal Year 1997: ¥8,700,000 (Direct Cost: ¥8,700,000)
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| Keywords | neuroblastoma / chromosome arm 14q / Positional cloning / LOH / 染色体第14番 / 染色体欠失 / マイクロサテライトマーカー / FISH / BAC |
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| Research Abstract |
We are investigating a tumor suppressor gene(s) responsible for neuroblastoma(NB) and we are focusing especially on chromosome arm 14q.As the first step in isolation and characterization of the putative tumor suppressor gene, we constructed a deletion map on chromosome band 14q32 by LOH study using 12 microsatellite markers.
In this study, we analyzed 54 tumors and corresponding constitutional tissues of patients with NB.We performed PCR-LOH study using microsatellite markers on 14q32. Seventeen (3 1%) of 54 cases showed loss of heterozygosity on chromosome band 14q32, and an approximately 1-cM region of common deletion was found that was flanked by D14S62 and D14S987. These results were confirmed by FISH analyses using bacterial artificial chromosome (BAG) purchased by this grant and/or cosmid clones isolated from human genomic cosmid library we constructed.
Now, we have constructed an approximately 8-cM sequence-ready contig consisting of BAG and cosmid clones that was flanked by D14S62 and D14S267 to isolate and analyze the putative tumor suppressor gene. Our findings support the *istence of putative tumor suppressor gene on 14q32 for the tumorigenesis of NB.Further investigations are required to elucidate the tumorigenesis of NB.
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Report
(3 results)
Research Products
(9 results)
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[Publications] Abe T., Makino, N., Furukawa, T., Ouyang, H., Kimura, M., Yatsuoka, T., Yokoyama, T., Inoue, H., Fukushige, S., Hoshi, M., Hayashi, Y., Sunamura, M., Kobari, M., Matsuno, S., and Horii, A.: "dentification of three commonly deleted regions on chromosome arm 6q in human pancreatic cancer" Genes Chromosomes Cancer.
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[Publications] Furukawa, T., Yatsuoka, T., E.M.Youssef, Abe, T., Yokoyama, T., Fukushige, S., Soeda, E., Hoshi, M., Hayashi, Y., Sunamura, M., Kobari, M., and Horii, A.: "Genomic analysis of DUSP6, a dual specificity MAP kinase phosphatase, in pancreatic cancer" Cytogenet.Cell Genet. 82. 156-159 (1998)
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[Publications] Yamakawa, H., Nagase, S., Yuki, M., Shiwaku, O.H., Furukawa, T., Yoshinaga, K., Soeda, E., Hoshi, M., Hayashi, Y., Sato, S., Yajima, A., and Horii, A.: "Identification of a 100-kb region of common allelic loss on chromosome bands 10q25-q26 in human endometrial cancer" Genes Chromosomes Cancer. 23. 74-77 (1998)
Description
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