| Project/Area Number |
09470386
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
小児外科
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| Research Institution | Tohoku University |
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Principal Investigator |
HAYASHI Yutaka Tohoku University School of Medicine Professor, 医学部, 教授 (40125638)
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| Co-Investigator(Kenkyū-buntansha) |
HORII Akira Tohoku University School of Medicine Professor, 医学部, 教授 (40249983)
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| Project Period (FY) |
1997 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥13,100,000 (Direct Cost: ¥13,100,000)
Fiscal Year 1998: ¥4,400,000 (Direct Cost: ¥4,400,000)
Fiscal Year 1997: ¥8,700,000 (Direct Cost: ¥8,700,000)
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| Keywords | neuroblastoma / chromosome arm 14q / Positional cloning / LOH / 染色体第14番 / 染色体欠失 / マイクロサテライトマーカー / FISH / BAC |
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| Research Abstract |
We are investigating a tumor suppressor gene(s) responsible for neuroblastoma(NB) and we are focusing especially on chromosome arm 14q.As the first step in isolation and characterization of the putative tumor suppressor gene, we constructed a deletion map on chromosome band 14q32 by LOH study using 12 microsatellite markers.
In this study, we analyzed 54 tumors and corresponding constitutional tissues of patients with NB.We performed PCR-LOH study using microsatellite markers on 14q32. Seventeen (3 1%) of 54 cases showed loss of heterozygosity on chromosome band 14q32, and an approximately 1-cM region of common deletion was found that was flanked by D14S62 and D14S987. These results were confirmed by FISH analyses using bacterial artificial chromosome (BAG) purchased by this grant and/or cosmid clones isolated from human genomic cosmid library we constructed.
Now, we have constructed an approximately 8-cM sequence-ready contig consisting of BAG and cosmid clones that was flanked by D14S62 and D14S267 to isolate and analyze the putative tumor suppressor gene. Our findings support the *istence of putative tumor suppressor gene on 14q32 for the tumorigenesis of NB.Further investigations are required to elucidate the tumorigenesis of NB.
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