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THE INVESTIGATION CONCERNING THE SUITABLE SITE FOR GRAFT IN LIVING-RELATED SMALL INTESTINAL TRANSPLANTATION USING MOLECULAR BIOLOGICAL METHOD

Research Project

Project/Area Number 09470388
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 小児外科
Research InstitutionKYUSHU UNIVERSITY

Principal Investigator

TAGUCHI Tomoaki  KYUSHU UNIVERSITY, MATERNAL AND PERINATALCARE UNIT,ASSOCIATE PROFESSOR, 医学部・附属病院, 助教授 (20197247)

Co-Investigator(Kenkyū-buntansha) HIROSE Ryuichiro  KYUSHU UNIVERSITY, PEDIATRIC SURGERY, RESEARCH ASSOCIATE, 医学部・附属病院, 助手 (90274753)
SHONO Takeshi  KYUSHU UNIVERSITY, PEDOATRIC SURGERY, ASSISTANT PROFESSOR, 医学部・附属病院, 講師 (30235717)
SUITA Sachiyo  KYUSHU UNIVERSITY, PEDIATRIC SURGERY, PROFESSOR, 医学研究院, 教授 (30038856)
NAKAO Makoto  KYUSHU UNIVERSITY, PEDIATRIC SURGERY, FELLOW, 医学部・附属病院, 医員
TAJIRI Tatsuro  KYUSHU UNIVERSITY, PEDIATRIC SURGERY, RESEARCH ASSOCIATE, 医学研究院, 助手 (80304806)
中村 晶俊  九州大学, 医学部, 医員
藤井 善充  九州大学, 医学部, 医員
梁井 桂子  九州大学, 医学部, 医員
山田 耕治  九州大学, 医学部, 医員
Project Period (FY) 1997 – 2000
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥4,900,000 (Direct Cost: ¥4,900,000)
Fiscal Year 2000: ¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1999: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1998: ¥1,600,000 (Direct Cost: ¥1,600,000)
Keywordsintestinal transplantation / jejunum / ileum / adaptation / c-fos / c-jun / mitosis / apoptosis / ブタ / ラット / 生体移植 / C-FOS / C-TUN / 部分小腸移植 / サイトカイン / 拒絶反応 / 分子生物学
Research Abstract

The suitable site as a graft of living-related, as well as, cadaveric small intestinal transplantation was investigated from the aspects of molecular biology.
The syngeneic segmental intestinal transplantation was performed using Lewis to Lewis rat combination. Intestinal tissues were taken from after 0 minute, 30 minutes, 4 hours, 24 hours, and 72 hours after transplantation. The expression of c-Fos and c-Jun, immediate earl genes, was examined as parameters of adaptation, using western blotting method. The results of jejunal grafts were compared with those of ileal grafts.
The c-Fos expression was seen 4 hours after transplantation, and the ileal grafts showed stronger and earlier expression than the jejunal grafts. This expression continued until 24 hours and disappeared at 72 hours. The c-Jun expression was seen only 4 hours after transplantation, and then disappeared at 24 hours. The ileal grafts also showed stronger expression than the jejunal grafts.
These results suggested that the ileum has bigger adaptation capacity than the jejunum in small intestinal transplantation.
Furthermore, the expression of c-fos and c-jun mRNA was measured after ischemia-reperfusion (I/R) in rat model, using RT-PCR. The c-fos expression was activated transiently, came to a peak at 30 minutes after reperfusion, and immediately declined. Activation of c-jun was slower, peaked at 90 minutes after reperfusion, and its expression prolonged longer than c-fos. The number of PCNA positive cells increased at 60 to 360 min. after reperfusion. And TUNEL positive cells increased later than PCNA positive cells, at 120 to 360 minutes after reperfusion. After I/R stress, transcriptional activation of c-fos and c-jun was observed in the small intestinal epithelium. Sequential expression patterns of those genes are thought to be related to the cellular response such as proliferation and apoptosis.

Report

(5 results)
  • 2001 Final Research Report Summary
  • 2000 Annual Research Report
  • 1999 Annual Research Report
  • 1998 Annual Research Report
  • 1997 Annual Research Report
  • Research Products

    (46 results)

All Other

All Publications (46 results)

  • [Publications] Nakao M, Ogita K, Shima Y, Taguchi T, Suita S, et al.: "Technical aspects of pig model in living-related small intestinal transplantation"Asian J Surgery. 24. 353-356 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ogita K, Suita S, Taguchi T, Yamanouchi T, Tsukimori K, Nakano H: "Experience of intrauterine treatment to fetal cystic hygroma : A report of two cases"Asian J Surgery. 23. 315-317 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Masumoto K, Nada O, Suita S, Taguchi T, Guo R: "The formation of the chick ileal muscle layers as revealed by α-smooth muscle actin immunohistochemistry"Anat Embryol. 201. 121-129 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Taguchi T, Fujii Y, Nakao M, Ogita K, Shima Y, Suita S: "Expression of "Immediate-early genes" c-Fos and c-Jun in small intestinal transplantation"Transplant Proc. 32(6). 1279 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nakao M, Shima Y, Yanai K, Taguchi T, Suita S: "Immune tolerance induced by administration of donor specific antigen and cyclophosphamide in rat fetal small bowel transplantation"Transplant Proc. 32(6). 1252 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Taguchi T, Suita S, Nakao M, Yamanouchi T, Inaba S: "The efficacy of predeposited autologous blood transfusions in general pediatric surgery"Surgery Today. 30(9). 773-777 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nakao M, Taguchi T, Yanai K, Yamada T, Suita S: "Energy metabolism during cold ischemia and reperfusion in rat small intestinal transplantation : comparison of jejunal and ileal grafts"J Pediatr Surg. 32. 1675-8 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Guo RS, Nada O, Suita S, Taguchi T, Masumoto K: "The distribution of and co-localization of nitric oxide synthase and vasoactive intestinal polypeptide in nerves of the colons with Hirschsprung's disease"Virchows Arch. 430. 53-61 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Taguchi T, Guo R, Masumoto K, Nada O, Rahman MS, Nakao M, Yanai K, Suita S: "Distribution of nitric oxide neurons after small intestinal transplantation"Transplant Proc. 30. 2626 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Taguchi T, Nakao M, Yanai K, Rahman MS, Yamada T, Suita S.: "Comparison of jejunal and ileal grafts -from the aspects of cold preservation and immunological reaction-"Transplant Proc. 30. 2636-7 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Rahman MS, Taguchi T, Masumoto K, Guo R, Nakao M, Yanai K, Suita S, Nada O: "Efficacy of cytological examination of luminal exudate in monitoring acute rejection of rat intestinal allografts"Transplant Proc. 30. 2653 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Rahman MS, Taguchi T, Masumoto K, Guo R, Suita S: "Monitoring of acute allograft rejection by cytological, immunocytochemical, and immunohistochemical studies following rat small-bowel transplantation"Surgery Today. 28. 529-37 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Taguchi T, Guo R, Masumoto K, Nada O, Nakao M, Yanai K, Suita S: "Chronological change of distribution of nitric oxide and peptidergic neurons after small intestinal transplantation"J Pediatr Surg. 34. 341-5 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Masumoto K, Suita S, Nada O, Taguchi T, Guo R: "Abnormalities of enteric neurons, intestinal pacemaker cells, and smooth muscle in human intestinal atresia"J Pediatr Surg. 34. 1463-8 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Masumoto K, Suita S, Nada O, Taguchi T, Guo R, Yamanouchi T: "Alterations of the intramural nervous distributions in a chick intestinal atresia model"Pediatr Res. 45. 30-7 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nakao M, Shima Y, Yanai K, Taguchi T, Suita S: "Immnune tolerance induced by administration of donor specific antigen and cyclophosphamide in rat fetal small bowel transplantation"Transplant Proc. 32. 1252 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Taguchi T, Fujii Y, Nakao M, Ogita K, Shima Y, Suita S: "Expression of "Immediate-early genes" c-Fos and c-Jun in small intestinal transplantation"Transplant Proc. 32. 1279 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Masumoto K, Nada O, Suita S, Taguchi T, Guo R: "The formation of the chick ileal muscle layers as revealed by α-smooth muscle actin immunohistochemistry"Anat Embryol. 201. 121-129 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nakao M, Ogita K, Shima Y, Taguchi T, Suita S, et al.: "Technical aspects of pig model in living-related small intestinal transplantation"Asian J Surgery. 24. 353-356 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nakao M,Ogita K,Shima Y,Taguchi T,Suita S, et al.: "Technical aspects of pig model in living-related small intestinal transplantation"Asian J Surgery. (accepted for publication).

    • Related Report
      2000 Annual Research Report
  • [Publications] Ogita K,Suita S,Taguchi T,Yamanouchi T,Tsukimori K,Nakano H: "Experience of intrauterine treatment to fetal cystic hygroma : A report of two cases"Asian J Surgery. 23. 315-317 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Masumoto K,Nada O,Suita S,Taguchi T,Guo R: "The formation of the chick ileal muscle layers as revealed by α-smooth muscle actin immunohistochemistry"Anat Embryol. 201. 121-129 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Taguchi T,Fujii Y,Nakao M,Ogita K,Shima Y,Suita S: "Expression of "Immediate-early genes" c-Fos and c-Jun in small intestinal transplantation."Transplant Proc. 32(6). 1279 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Nakao M,Shima Y,Yanai K,Taguchi T,Suita S: "Immune tolerance induced by administration of donor specific antigen and cyclophosphamide in rat fetal small bowel transplantation"Transplant Proc. 32(6). 1252 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Taguchi T,Suita S,Nakao M,Yamanouchi T,Inaba S: "The efficacy of predeposited autologous blood transfusions in general pediatric surgery."Surgery Today. 30(9). 773-777 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Taguchi T, Guo R, Masumoto K, Nada O, Nakao M, Yanai K, Suita S: "Chronological change of distribution of nitric oxide and peptidergic neurons after small intestinal transplantation"J, Pediatr Surg. 34・2. 341-345 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Masaumoto K, Suita S, Nada O, Taguti T, Guo R, Yamanouchi T: "Alterations of the intramural nervous distributions in a chick intestinal atresia model"Pediatr Res. 45・1. 30-37 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Nakao M, Shima Y, Yanai K, Taguchi T, Sita S: "Immune tolerance induced by administration of donor specific antigen and cyclophosphamide in rat fetal small bowel trnsplantation"Transplant Proc,. (in press).

    • Related Report
      1999 Annual Research Report
  • [Publications] Taguchi T, Fujii Y, Nakao M, Yanai K, Shima Y, Suita S: "Expression of "Immediate-early genes" c-Fos and c-Jun in small intestinal transplantation."Transplant Proc. (in press).

    • Related Report
      1999 Annual Research Report
  • [Publications] 田口智章、水田祥代、中村晶俊、中尾 真: "保存臓器とviability判定法:小腸"低温医学. 25・1. 42-48 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 田口智章、中尾 真、中村晶俊、水田祥代: "小腸移植における再灌流障害の発生機序(活性酸素の関与)"低温医学. 25・2. 97-103 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 田口智章、中尾 真、水田祥代: "臓器移植実験マニュアル-ラット・マウスを用いた移植、虚血再灌流障害モデル-第5章ラット異所性小腸移植(その1)"秀潤社 野澤眞澄監修. 234(133-139) (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Taguchi T. Guo R.Masumoto K.Nada O.Rahman MS.Nakao M.Yanai K Suita S: "Chronological change of distoribution of nitric oxide neurons aftar rat small intestinal transplantatiion" Transplant Proc. 30. 2626 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Taguchi T.Nakano M.Yanai K.Rahman MS.Yamada T.Suita S: "Comparison of jejunal and iteal grafts-from the aspects of cold preservation and immunological reaction" Transplant Proc. 30. 2636-2367 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Rahman T.Tguchti T.Masumoto K.Guo R.Nakao M.Yanai K.Suita S.Nada O: "Efficacy of cytological examination of luminal exudate in monitoring acute rejection of rat intestinal allgrafts" Transplant Proc. 30. 2653 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Taguchi T.Suita S.Yamanouchi T.Masumoto K.Yanai K: "Progress in treatment of esophageal atresia" proceedings of the 8th Fukuoka international Symposium on perinatal Medicine. 8. 43-50 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Rahman MS.Taguchi T.Masumoto K.Guo R.Suita S: "Monitoring of acuta allograft rejection by cytological.immunohistochemical studies fllowing rat small bowel transplantation" Surgery Today. 28. 529-537 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Suita S.Taguchi T.Yanai K.Kamimura T.Nakao M.Ikeda K: "Longterm outcomes and quality of life after Z-shaped anastomosis for Hirschsprung's disease" JAm Coll Surg. 187. 577-583 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Masumoto K.Suita S.Nada O.Taguchi T.Guo R.Yamanouchi T: "Pediatr Res 45" Alterationa of the intramural nervous distributions in a chick intestinal atresia model, 30-37 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Taguchi T.Guo R.Masumoto K.Nada O.Nakao M.Yanai K.Suita S: "JPediatr Surg 34" Chronological chenge of distribution in nitric oxide and peptidergic neurons after rat small intestinal, 341-345 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Gvo R,Nada O,Suita S,Taguchi T,Masumoto K: "The distribution and co-localization of nitric oxide synthase and vasoactive intestinal polypeptide in nerves of the colons with Hirschsprungs disease" Virchows Arch. 430. 53-61 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Nakao M, Taguchi T, Yanai K, Yamada T,Suita S: "Energy metabslism during cold is chemia and reperfusion in the rat small intestinal transplantation:comparison of jejunal and ileal grafts" J Pediatr Surg. 32. 1675-1678 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Suita S,Taguchi T,Kamimura T,Yanai K: "Total colonic aganglionosis with or without small bowel involvement:A changing profile" J Pediatr Surg. 32. 1537-1541 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 田口智章,中尾真,梁井桂子 Rahman MS,山田耕治,水田祥代: "ラット部分小腸移植における空調および回腸グラフトの比較" 今日の移植. 10. 71-78 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 田口智章,梁井桂子,中尾真,水田祥代: "冷保存小腸のviability判定法" 低温医学. 23. 5-11 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 田口智章,山田耕治,梁井桂子,水田祥代: "ラット小腸移植手技-異所性" Organ Biology. 4. 57-63 (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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