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The Analysis of Gene Expression of Morphogenetic Factors Implicated Development of Tooth Germ and Odontogenic Tumors

Research Project

Project/Area Number 09470391
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Morphological basic dentistry
Research InstitutionOkayama University

Principal Investigator

NAGAI Noriyuki  Okayama University, Dental School, Professor, 歯学部, 教授 (90085770)

Co-Investigator(Kenkyū-buntansha) ISHIWARI Yuzo  Okayama University, Dental School, Assistant, 歯学部, 助手 (10304312)
INOUE Masahisa  Okayama University, Dental School, Assistant, 歯学部, 助手 (20223274)
NAGATSUKA Hitoshi  Okayama University, Dental School, Associate Professor, 歯学部, 助教授 (70237535)
赤木 巧  岡山大学, 歯学部, 教務員 (50192878)
Project Period (FY) 1997 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥10,600,000 (Direct Cost: ¥10,600,000)
Fiscal Year 1999: ¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 1998: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1997: ¥4,100,000 (Direct Cost: ¥4,100,000)
Keywordstooth germ / morphogenesis / cytodifferentiation / basement membrane / type IV collagen / growth factor / TGF-β / FGF / 上皮間葉相互作用 / 成長因子 / 歯胚分化 / アメロジェニン / 遺伝子発現
Research Abstract

Tooth morphogenesis and cytodifferentiations are regulated by epithelium-mesenchymal interactions, and the basement membrane plays a role in mediating these interactions Type IV collagen, the major component of the dental basement membrane, is composed of three αchains. Using indirect immunofluorescence on cryosections, we characterized the changes in protein localization of α1 to α6 chains of type IV collagen during mouse molar development. We propose that the inner enamel epithlium in the cap stage is composed of type IV collagen consist of α1, α2 and α4 chains, probably in molecular forms of α1(IV) α2(IV) α4(IV), [α4(IV)]ィイD23ィエD2 and [α1(IV)]ィイD22ィエD2 α2(IV). These forms containing α4 chain have not been detected in other organs yet. Therefore, it is thought that these forms is related to morphogenesis and cytodifferentiation characteristic of tooth development that regulated y epithelial-mesenchymal interactions.
Recently, it has gradually been ascertained that some growth factors secreted by epithelial or mesenchymal tissue bind to type IV collagen with greater affinity, condens there and contribute to organ morphogenesis in cooperation with basement membrane. We observed the expression patterns of several growth factors (TGF-β, BMPs and FGFs) in the basement menbrane of mouse first molar during tooth germ development and explored the relation between these growth factors and the tooth germ-specific molecular forms of type IV collage. No examined growth factor was detected in the basement membrane in and after cap stage. On the other hand, in the dental lamina and bud stages FGF-2 and TGF-β1 were detected in the dental basement membrane. FGF-2 and TGF-β1 may play an important role in early tooth morphogenesis and cytodifferentiation in cooperation with the molecular form, α3(IV)α4(IV)α5(IV), which exsists in the early stage of tooth development.

Report

(4 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • 1997 Annual Research Report
  • Research Products

    (61 results)

All Other

All Publications (61 results)

  • [Publications] Nagatsuka, H.: "Gene expression of chondro-osseous cells in heterotopic bone formation induced by rhBMP-2"J. Hard Tissue Biol.. 6. 10-15 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Qin, C.L.: "Aging and ectopic bone formation induced by partially purified bone morphogenetic protein"Jpn. J. Oral Biol.. 39. 572-582 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Qin, C.L.: "Morphorogical and immunohistochemical study of ectopic bone formation induced by crude BMP in 4-week old and 6-month old rats"J. Jpn. Soc. Oral Implant.. 8. 288-295 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Qin, C.L.: "Aging and ectopic bone formation induced by partially purified bone morphogenetic protein : Blood vessel ingrowth and localization of type I collagen and osteocalcin assessed by immunohistochemistry"J. Hard Tissue Biol.. 6. 95-104 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Murata, M.: "Bone morphogenetic protein (BMP) stimulates cytological and functional differentiation of preodontoblasts in vitro"J. Hard Tissue Biol.. 6. 105-113 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kuboki, Y.: "BMP-induced osteogenesis on the surface hydroxyapatite with geometrical feasible and nonfeasible structure : Topology of osteogenesis"J. Biomed. Mater. Res.. 39. 190-199 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Shin, H.I.: "The effect of BMP on the monocyte-macrophage-mesenchymal cell interaction in ectopic one formation"J. Hard Tissue Biol.. 7. 11-15 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Tan, J.: "Long-term observation of heterotopic bone formation induced by rhBMP-2 and IBM composites"J. Hard Tissue Biol.. 7. 16-20 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Mizuhira, V.: "Demonstration of calcium ion distribution in calcifying cells. -X-ray microanalysis and electron spectroscopic imaging after fixation with NHA-containing fixatire and microwave irradiation-"Acta Histochem. Cytochem.. 31. 217-230 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Murata, M.: "Carrier-dependency of cellular differentiation induced by bone morphogenetic protein in ectopic sites"Int. J. Oral Maxillofac. Surg.. 27. 391-396 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Nagatsuka, H.: "Bone augmentation by BMP-collagen I and BMP-porous block hydroxapatite composite in RAT calvarial subperiosteum"Bioceramics. 11. 549-552 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Nagai, N.: "Multiple initial calcification in ectopic bone formation induced by a BMP-collagen composite"Bioceramics. 11. 553-555 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Takagi, M.: "pH and carbonate levels in developing enamel"Connective Tissue Research. 38. 181-187 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Murata, M.: "Bone augmentation by recombinant human BMP-2 and collagen on adult rat paricetal bone"Int. J. Oral Maxillofac. Surg.. 28. 232-237 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Nagai, N.: "Age effects on ectopic bone formation induced by purified bone morphogenetic protein"Int. J. Oral Maxillofac. Surg.. 28. 143-150 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Nagai, N.: "Immunohistochemical Localization of Human Epidermal Growth Factor Receptor on Carcinogenesis in Human Oral Mucosa"J. Okayama Dent. Soc.. 18. 21-26 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 中野敬介: "マウス歯胚基底膜におけるIV型コラーゲンα鎖の消長に関する免疫組織学的研究"岡山歯学会雑誌. 18. 69-82 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kishi, T.: "Histological Study of Heterotopic Bone Formation and Growth Induced by rhBMP-2 and IBM Composites"J. Jpn. Soc. Oral Implant.. 12. 206-212 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Nagai, N.: "Histo-pathological Study of Bone Formation Using Porous Hydroxyapatite-BMP Composite in Dog Jaw Bone Defect"Bioceramics. 12. 229-231 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Inoue, M.: "Long-term Observation of Heterotopic Bone Formation Induced by rhBMP-2 and IBM Composites"Bioceramics. 12. 261-264 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 永井教之 監修: "形態形成・分子メカニズム研究の最新技術"財団法人 口腔保健協会. 250 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 永井教之 監修: "歯のできかたのしくみ その分子生物学的展望"Journal of Hard Tissue Biology Vol.8 Special Issue. 313 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] H. Nagatsuka: "Gene expression of chondro0-osseous cells in heterotopic bone formation induced by rhBMP-2"J. Hard Tissue Biol.. Vol.6(1). 10-15 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] C. L. Qin: "Aging and ectopic bone formation induced by partially purified bone morphogenetic protein"Jpn. J. Oral Biol.. Vol.39(5(. 572-582 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] C. L. Qin: "Morphorogical and immunohistochemical study of ectopic bone formation induced by crude BMP in 4-week old and 6-month old rats"J. Jpn. Soc. Oral Implant.. Vol.8(3). 288-295 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] C. L. Qin: "Aging and ectopic bone formation induced by partially purified bone morphogenetic protein : Blood vessel ingrowth and localization of type I collagen and osteocalcin assessed by immunohistochemistry"J. Hard Tissue Biol.. Vol.6(3). 95-104 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] M. Murata: "Bone morphogenetic protein (BMP) stimulates cytological and functional differentiation of preodontoblasts in vitro"J. Hard Tissue Biol.. Vol.6(3). 105-113 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Y. Kuboki: "BMP-induced osteogenesis on the surface hydroxyapatite with geometrical feasible and nonfeasible structures : Topology of osteogenesis"J. Biomed. Mater. Res. Vol.39. 190-199 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] H. I. Shin: "The effect of BMP on the monocyte-macrophage-mesenchymal cell interaction in ectopic bone formation"J. Hard Tissue Biol.. Vol.7(1). 11-15 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] J. Tan: "Long-term observation of heterotopic bone formation induced by rhBMP-2 and IBM composites"J. Hard Tissue Biol.. Vol.7(1). 16-20 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] V. Mizuhira: "Demonstration of calcium ion distribution in calcifying cells. -X-ray microanalysis and electron spectroscopic imaging after fixation with NHA-containing fixatire and microwave irradiation-"Acta Histochem. Cytochem.. Vol.31(3). 217-230 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] M. Murata: "Carrier-dependency of cellular differentiation induced by bone morphogenetic protein in ectopic sites"Int, J, Oral Maxillofac. Surg.. Vol.27. 391-396 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] H. Nagatsuka: "Bone augumentation by BMP-collagen I and BMP-porous block hydroxyapatite composite in RAT calvarial subperiosteum"Bioceramics. Vol.11. 549-552 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] N. Nagai, R. Tamamura, R. Z. LeGeros: "Multipul initial calcification in ectopic bone formation induced by a BMP-collagen composite"Bioceramics. Vol.11. 553-555 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] T. Takagi: "pH and carbonate levels in developing enamel"Connective Tissue Research. Vol.38(1-4). 181-187 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] M. Murata: "Bone augmentation by recombinant human BMP-2 and collagen on adult rat paricetal bone"Int. J. Oral Maxillofac. Surg.. Vol.28. 232-237 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] N. Nagai: "Age effects on ectopic bone formation induced by purified bone morphogenetic protein"Int. J. Oral Maxillofac. Surg.. Vol.28. 143-150 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] N. Nagai: "Immunohistochemical Localization of Human Epidermal Growth Factor Receptor on Carcinogenesis in Human Oral Mucosa"J. Okayama Dent. Soc.. Vol.18(1). 21-26 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] T. Kishi: "Histological Study of Heterotopic Bone Formation and Growth Induced by rhBMP-2 and IBM Composites"J. Jpn. Soc. Oral Implant.. Vol.12(2). 206-212 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] N. Nagai: "Histo-pathological Study of Bone Formation Using Porous Hydroxyapatite-BMP Composite in Dog Jaw Bone Defect"Bioceramics. Vol.12. 229-231 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] M. Inoue: "Long-term Observation of Heterotopic Bone Formation Induced by rhBMP-2 and IBM Composites"Bioceramics. Vol.12. 261-264 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] N. Nagai: "Frontier of biological technology on morphogenesis and its molecular mechanism"Oral Health Association of Japan. 1-250 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] N. Nagai: "Outlook of molecular mechanism on odontogenesis"J. Hard Tissue Biol. Vol.8 Special Issue. 1-313 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Takagi T.: "pH Carbonate Levels in Developing Enamel"Connective Tissue Research. 38. 181-187 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 中野敬介: "マウス歯胚基底膜におけるIV型コラーゲンα鎖の消長に関する免疫組織学的研究"岡山歯学会誌. 18・1. 69-82 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Nagai N.: "Age effects on ectopic bone formation induced by purified bone morphogenetic protein"International Journal of Oral and Maxillofacial Surgery. 28. 143-150 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Murata M.: "Bone augmentation by recombinant human BMP-2 and collagen on adult rat partial bone"International Journal of Oral and Maxillofacial Surgery. 28. 232-237 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 永井教之: "実験的研究からみた歯の発生メカニズムの文献的考察"Journal of Hard Tissue Biology. 8. 1-8 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 中野敬介: "歯胚発生時におけるエナメル基質蛋白(アメロゲニン)の遺伝子発現について" 日本機械学会代10回バイオエンニジアリング講演会論文集. 97-72. 551-552 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 石割裕三: "In situ Hybridization法を用いた硬組織におけるコラーゲンmRNAの検出" 形態形成,分子分子メカニズム研究の最新技術. 81-85 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Vinci Mizuhira: "Demonstration of Calcium Ion Distribution in Calcifying Cells" Acta Histochem Cytochem. 7-1. 217-230 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 中野敬介: "マウス歯胚基底膜におけるIV型コラーゲンα鎖の消長に関する免疫組織学的研究" 岡山歯学会雑誌. 18-1. (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Nagatsuka N.: "Gene expression of chondro-osseous cells in heterotopic bone formation induced by rhBMP-2" J.Hard Tissue Biol.6(1). 10-15 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 完山 学: "部分精製骨形成タンパク質による異所性骨誘導過程における骨基質タンパク遺伝子の発現に関する研究" 岡山歯学会誌. 16(1). 49-69 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 此内 浩信: "骨形成タンパク質による異所性硬組織誘導とI型,II型,X型コラーゲン遺伝子の発現-In situハイブリダイゼーション法による検討-" 岡山歯学会誌. 16(1). 117-132 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Shin H.I.: "Verruciform xanthoma of the oral mucosa:An immunohistochemical and ultrastructural study of two cases" Oral Oncology. 33(4). 279-283 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Qin C.L.: "Aging and ectopic bone formation induced by partially purified bone morphogenetic protein" Jpn.J.Oral Biol.39(5). 572-582 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Qin C.L.: "Morphological and immunohistochemical study of ectopic bone formation induced by crude BMP in 4-week old and 6-month old rats" 日本口腔インプラント学会誌. 8(3). 288-295 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Qin C.L.: "Aging and ectopic bone formation induced by partially purified bone morphogenetic protein:blood vessel ingrowth and localization of type I collagen and osteocalcin assessed by immunohistochemistry" J.Hard Tissue Biol.6(3). 95-104 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Murata M.: "Bone morphogenetic protein(BMP)stimulates cytological and functional differentiation of preodontoblasts in vitro" J.Hard Tissue Biol.6(3). 105-113 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 中野 敬介: "歯胚発生時におけるエナメル基質タンパク質(アメロゲジェニン)の遺伝子発現について" 日本機会学会第10回バイオエンジニアリング講演会論文集. 97-72. 551-552 (1998)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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