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Antitumor activity of Porphyromonas gingivalis lipid A

Research Project

Project/Area Number 09470453
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Surgical dentistry
Research InstitutionOsaka University

Principal Investigator

SAKUDA Masayoshi  Faculty of Dentistry, Professor, 歯学部, 教授 (00028755)

Co-Investigator(Kenkyū-buntansha) IWAI Souichi  University Dental Hospital, Senior resident, 歯学部・附属病院, 医員
KATO Itsuro  University Dental Hospital, Senior resident, 歯学部・附属病院, 医員
NAKAZAWA Mitsuhiro  University Dental Hospital, Assistant professor, 歯学部・附属病院, 講師 (70217701)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥7,600,000 (Direct Cost: ¥7,600,000)
Fiscal Year 1998: ¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1997: ¥5,500,000 (Direct Cost: ¥5,500,000)
KeywordsPorphyromonas gingivalis381 / Lipid A / LPS / cytokines / macrophage / cytostatic & cytolytic activity / metastasis / lymph node / OK-432 / NK activity / P.gingivalis381 LipidA / P.gingivalis381 LPS / マクロファージ / cytostatic & cytolytic activity
Research Abstract

We investigated the antitumor activity and immunomodulatory effects of P.gingivalis lipid A.Periphera blood mononuclear cells obtained from healthy donors were incubated with Porphyromonas gingivalis lipid A(5Omcg/ml) in vitro. The supernatants were harvested and measured the activities of cytokines such as IL-1gamma, IL-1 receptor antagonist (IL-1ra), IL-6, IL-8, GM-CSF.TNF-alpha and IFN-gamma by ELISA assay. P.gingivalis lipid A induced considerably weaker IL-1gamma and TNF-alpha production than did E.coil LPS and its lipid A and salmonella-type lipid A.ft gingivalis lipid A induced higher IL-1ra production than did F.coil LPS and its lipid A.P.gingivalis LPS and its lipid A induced high levels of IL-6, IL-8 and GM-CSF.IFN-gamma production was more strongly induced by Rgingivalis LPS than by the other test specimens.
Cytostasis by splenic macrophages stimulated with ft gingivails LPS and its lipid A were enhanced significantly in a dose-dependent manner. In the cytolytic assay, ft gingivalis lipid A exhibited tumoricidal activities, but weaker than its parent LPS.
NK activities of P.gingivalis LPS and its lipid A were enhanced and almost equivalent to those obtained with E coil LPS and its lipid A.
P.gingivails lipid A were failed to inhibit regional lymph node metastasis of MM48 mammary carcinoma. P.gingivalis LPS most strongly inhibited the lymph nodes metastases.
These results suggested that ft gingivails lipid A had different immunological activities from those of enterobacterial lipid As, and induced enough level of antitumor immunity. P.gingivalis lipid A has a possibility of clinical use as a low-toxic immunomodulator, but it is necessary to investigate the effective administration methods in clinical model.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] 作田正義: "口腔外科の卒前臨床実習の現状と口腔外科領域における技能・態度に関する評価-アンケート調査の結果について-" 日本歯科医学教育学会雑誌. 13(1). 113-120 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Shukunami C.: "Sequential progression of the differentiation program by bone morphogenetic protein-2 in chondrogenic cell line ATDC5" Exp.Cell Res.241. 1-11 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 中澤光博: "口腔扁平上皮癌再発症例における組織学的悪性度の変化について-外科療法と放射線療法の比較-" 頭頚部腫瘍. 24(1). 29-33 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nakazawa M.: "Central acinic cell carcinoma of mandible Report of a case" Int.J.Oral Maxillofac.Surg.27(6). 448-450 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kawai T.: "Gross periostitis ossifications in mandibular osteomyelitis. Review of the english literature and radiographic variation" Oral Surg.Oral Med.Oral Pathol. Oral radiol.Endod.86. 376-382 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kawai T.: "Diagnostic imaging in two cases of recurrent maxillary ameloblastoma : comparative evaluation of plain radiographs,CT and MR images" Br.J.Oral Maxillofac.Surg.36. 304-310 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Masayoshi Sakuda: "The situation of clinical practice of undergraduate-education and valuation on operational skill and behavior in oral surgery-The results of a questionnaire-" The Journal of Japanese Association for Dental Education. 13(1). 113-120 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Chisa Shukunami, Yoshiyuki Ohta, Masayoshi Sakuda Yuji Hiraki: "Sequential progression of the differentiation program by bone morphogenetic protein-2 in chondrogenic cell line ATDC5" Exp.Cell Res.241. 1-11 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Mitsuhiro Nakazawa, Souichi Iwai, Tatsushi Matsumura, Hiroshi Tamura, Shigeru Moriga, Yasuhiro Uekusa Rifat Hasina, Masayoshi Sakuda: "The changes of histological malignancy in recurrent squamous cell carcinoma of oral cavity. Comparison between surgery and radiotherapy-" Head and Neck Cancer. 24(1). 29-33 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Mitsuhiro Nakazawa, Tetsurou Ohnishi, Souichi Iwai Masayoshi Sakuda.: "Central acinic cell carcinoma of the mandible report of a case" Int.J.Oral.Maxillofac.Surg.27. 448-450 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tadahiko Kawai, Hiroko Hiranuma, Mitsunobu Kishino, Shumei Murakami, Masayoshi Sakuda, Hajime Fuchihata: "Gross periostitis ossificans in mandibular osteomyelitis. Review of the English literature and radiographic variation" Oral Surg.Oral Med.Oral Pathol Oral Radiol Endod.86. 376-382 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tadahiko Kawai, Shumei Murakami, Mitsunobu Kishino, Tokuzo Matsuya, Masayoshi Sakuda, Hajime Fuchihata.: "Diagnostic imaging in two cases of recurrent maxillary ameloblastoma : Comparative evaluation of plain radiographs, CT and MR images." Br J Oral Maxillofac Surg. 36. 304-310 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Shukunami C.: "Sequential progression of the differentiation program by bone morphogenetic protein-2 in chondrogenic cell line ATDC5" Exp.Cell Res.241. 1-11 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 中澤光博: "口腔扁平上皮癌再発症例における組織学的悪性度の変化について-外科療法と放射線療法の比較-" 頭頚部腫瘍. 24(1). 29-33 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Nakazawa M.: "Central acinic cell carcinoma of mandible Report of a case" Int.J.Oral Maxillofac.Surg.27(6). 448-450 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kawai T.: "Gross periostitis ossifications in mandibular osteomyelitis.Review of the english literature and radiographic variation" Oral Surg.Oral Med.Oral Pathol.Oral radiol.Endod.86. 376-382 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kawai T.: "Diagnostic imaging in two cases of recurrent maxillary ameloblastoma:comparative evaluation of plain radiographs,CT and MR images" Br.J.Oral Maxillofac.Surg.36. 304-310 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 作田正義: "口腔外科の卒前臨床実習の現状と口腔外科領域における技能・態度に関する評価" 日本歯科医学教育学会雑誌. 13(1). 113-120 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 額田純一郎: "外傷による脱落永久歯36歯の再植に関する臨床的検討。" 日本口腔外科学会雑誌. 43(10). 751-753 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 吉川文弘: "口蓋小唾液腺原発腫瘍の臨床的検討" 日本口腔科学会雑誌. 46(1). 55-59 (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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