| Project/Area Number |
09470457
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | The University of Tokushima |
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Principal Investigator |
NAGAYAMA Masaru The University of Tokushima, School of Dentistry, Professor, 歯学部, 教授 (30022867)
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| Co-Investigator(Kenkyū-buntansha) |
YAMANOUCHI Kohji The University of Tokushima, School of Dentistry, Research Associate, 歯学部, 助手 (30294705)
FUJISAWA Kenji The University of Tokushima, School of Dentistry, Research Associate, 歯学部, 助手 (40228979)
HAYASHI Eiji The University of Tokushima, University Dental Hospital Assistant Professor, 歯学部附属病院, 講師 (50173000)
KAMATA Nobuyuki The University of Tokushima, School of Dentistry, Associate Professor, 歯学部, 助教授 (70242211)
布袋屋 智朗 徳島大学, 歯学部・附属病院, 助手 (10274234)
中野 孝三郎 徳島大学, 歯学部・附属病院, 助手 (20274235)
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| Project Period (FY) |
1997 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥12,300,000 (Direct Cost: ¥12,300,000)
Fiscal Year 1998: ¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 1997: ¥8,800,000 (Direct Cost: ¥8,800,000)
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| Keywords | oral SCC / secretion / cytokine / protein-free medium / cell proliferation |
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| Research Abstract |
We have succeeded in culturing many human cancer cells including oral squamous cell carcinoma (SCC) cells in a protein-free synthetic medium, PF86-1. However, there are some human cancer cells that can't proliferate under protein-free conditions, It is contemplated that the essential factors for the growth may be lacking. In this study, it was investigated whether cytokines could act as a growth stimulator for oral squamous cell carcinoma cells cultured under protein-free conditions. As a result, IL-1a acts as a growth stimulator for oral squamous cell carcinoma cells by autocrine and paracrine mechanisms.
Recently, another novel protein-free synthetic medium, PFM-7 has been developed by us for the culture of normal human oral keratinocytes. Normal oral keratinocytes growing in PFM-7 exhibited nearly equal potency to grow in culture without changes in morphology, response to the added growth factors and gene expression of growth factors and their receptors when compared with the cells cultured in the conventional medium containing growth factors. Using these protein-free conditions, a comparative study of gene expression of SCC cell lines and normal oral keratinocytes derived from the same patient was performed.
Subtractive cDNA libraries of normal and malignant cells were constructed and specific genes which are increased or decreased in cancer cells compared to the normal oral keratinocytes of the same patient were isolated. One of the genes which are suppressed in cancer cells had a homology of S100 protein and is decreased in several SCC cell lines in RNA blot hybridization analysis. Functions of the gene are analized now.
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