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Effects of Cytokines on the Proliferation Mechanism of Oral Cancer and Oral Epithelial Cells

Research Project

Project/Area Number 09470457
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Surgical dentistry
Research InstitutionThe University of Tokushima

Principal Investigator

NAGAYAMA Masaru  The University of Tokushima, School of Dentistry, Professor, 歯学部, 教授 (30022867)

Co-Investigator(Kenkyū-buntansha) YAMANOUCHI Kohji  The University of Tokushima, School of Dentistry, Research Associate, 歯学部, 助手 (30294705)
FUJISAWA Kenji  The University of Tokushima, School of Dentistry, Research Associate, 歯学部, 助手 (40228979)
HAYASHI Eiji  The University of Tokushima, University Dental Hospital Assistant Professor, 歯学部附属病院, 講師 (50173000)
KAMATA Nobuyuki  The University of Tokushima, School of Dentistry, Associate Professor, 歯学部, 助教授 (70242211)
布袋屋 智朗  徳島大学, 歯学部・附属病院, 助手 (10274234)
中野 孝三郎  徳島大学, 歯学部・附属病院, 助手 (20274235)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥12,300,000 (Direct Cost: ¥12,300,000)
Fiscal Year 1998: ¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 1997: ¥8,800,000 (Direct Cost: ¥8,800,000)
Keywordsoral SCC / secretion / cytokine / protein-free medium / cell proliferation
Research Abstract

We have succeeded in culturing many human cancer cells including oral squamous cell carcinoma (SCC) cells in a protein-free synthetic medium, PF86-1. However, there are some human cancer cells that can't proliferate under protein-free conditions, It is contemplated that the essential factors for the growth may be lacking. In this study, it was investigated whether cytokines could act as a growth stimulator for oral squamous cell carcinoma cells cultured under protein-free conditions. As a result, IL-1a acts as a growth stimulator for oral squamous cell carcinoma cells by autocrine and paracrine mechanisms.
Recently, another novel protein-free synthetic medium, PFM-7 has been developed by us for the culture of normal human oral keratinocytes. Normal oral keratinocytes growing in PFM-7 exhibited nearly equal potency to grow in culture without changes in morphology, response to the added growth factors and gene expression of growth factors and their receptors when compared with the cells cultured in the conventional medium containing growth factors. Using these protein-free conditions, a comparative study of gene expression of SCC cell lines and normal oral keratinocytes derived from the same patient was performed.
Subtractive cDNA libraries of normal and malignant cells were constructed and specific genes which are increased or decreased in cancer cells compared to the normal oral keratinocytes of the same patient were isolated. One of the genes which are suppressed in cancer cells had a homology of S100 protein and is decreased in several SCC cell lines in RNA blot hybridization analysis. Functions of the gene are analized now.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report

Research Products

(17 results)

All Other

All Publications (17 results)

  • [Publications] H Nakanishi: "The association of platelet-derived growth factor (PDGF) receptor tyrosine phosphorylation to mitogenic response of human osteoblasic cells in vitro." Oral Diseases. 3. 236-242 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 林 英司: "同一患者から樹立した3種の口腔癌細胞株とその性状" 日口外誌. 43・11. 789-796 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 桃田幸弘: "口腔扁平上皮癌細胞の増殖に及ぼすサイトカインの影響" 四国歯誌. 11・1. 139-159 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 藤澤健司: "ウサギ口腔粘膜における創傷治癒遅延モデルの作製" 日口粘膜誌. 4・1. 54-62 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Toshiaki Hoteiya: "Expression of E-cadherin in oral cancer cell lines and its relationship to invasiveness in SCID mice in vivo." J.Oral Pathol.Med.印刷中. (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] H Nakanishi: "The association of platelet-derived growth factor (PDGF) receptor tyrosine phosphorylation to mitogenic response of human osteoblastic cells in vitro" Oral Diseases. 3. 236-242 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Eiji Hayashi: "Three oral cancer cell lines established from a patient and their characterization" Jpn. Oral Maxillofac. Surg.43-11. 789-796 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yukihiro MOMOTA: "Effects of Cytokines on the Proliferation of Oral Squamous Cell Carcinoma Cells" Shikoku Dent. Res.11-1. 139-159 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kenji FUJISAWA: "Design of Model for Impaired Healing of Wound Created on Oral Mucosa of Rabbits" J.Jpn. Oral Muco. Membr.4-1. 54-62 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Toshiaki Hoteiya: "Expression of E-cadherin in oral cancer cell lines and its relationship to invasiveness in SCID mice in vivo." J.Oral Pathol. Med.(in press). (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] H Nakanishi: "The association of platelet-derived growth factor(PDGF)receptor tyrosine phosphorylation to mitogenic response of human ostcoblastic cells in vitro." Oral Diseases. 3. 236-242 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] 林 英司: "同一患者から樹立した3種の口腔癌細胞株とその性状" 日口外誌. 43・11. 789-796 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] 桃田幸弘: "口腔扁平上皮癌細胞の増殖に及ぼすサイトカインの影響" 四国歯誌. 11・1. 139-159 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 藤澤健司: "ウサギ口腔粘膜における創傷治癒遅延モデルの作製" 日口粘膜誌. 4・1. 54-62 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Toshiaki Hoteiya: "Expression of E-cadherin in oral cancer cell lines and its relationship to invasiveness in SCID mice in vivo." J.Oral Pathol.Med.(印刷中). (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] 林 英司: "同一患者から樹立した3種の口腔癌細胞株とその性状" 日本口腔外科学会雑誌. 43・11. 789-796 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 桃田幸弘: "口腔扁平上皮癌細胞の増殖に及ぼすサイトカインの影響" 四国歯学会雑誌. 11・1(印刷中). (1998)

    • Related Report
      1997 Annual Research Report

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Published: 1997-03-31   Modified: 2016-04-21  

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