| Project/Area Number |
09470486
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Chemical pharmacy
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| Research Institution | KYUSHU UNIVERSITY |
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Principal Investigator |
HIGUCHI Ryuichi Kyushu University, Graduate Scholl of Phamaoeutical Sciences, Professor, 薬学研究科, 教授 (60122727)
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| Co-Investigator(Kenkyū-buntansha) |
INAGAKI Masanori Kyushu University, Graduate Scholl of Phamaoeutical Sciences, Assistant, 薬学研究科, 助手 (90274480)
YAMADA Koji Kyushu University, Graduate Scholl of Phamaoeutical Sciences, Assistant, 薬学研究科, 助手 (00253469)
MIYAMOTO Tomofumi Kyushu University, Graduate Scholl of Phamaoeutical Sciences, Associate Professor, 薬学研究科, 助教授 (40182050)
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| Project Period (FY) |
1997 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥12,200,000 (Direct Cost: ¥12,200,000)
Fiscal Year 1999: ¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 1998: ¥2,700,000 (Direct Cost: ¥2,700,000)
Fiscal Year 1997: ¥6,900,000 (Direct Cost: ¥6,900,000)
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| Keywords | Echinoderms / Starfish / Sea Cucumber / Sea Lily / Glycosphingolipid / Isolation / Structure Determination / Biological Activities |
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| Research Abstract |
In the echinoderms such as starfish and se cucumber, the presence of biologically active glycosphingolipids are excepted. In this time, we conducted the isolation and structure determination of the biologically active glycosphingolipids from echinoderms with the object of searching the new medical resources. The results were as follows.
1. Starfish : (1) Two cerebrosides, two ceramide lactosides, one sulfatide, and four gangliosides were isolated from Luidia maculata and the structures were determined. (2) From Linckia laevigata, two gangliosides were obtained and characterized. (3) One gangliosides was isolated and the structure was elucidated from Acanthaster planci.
2. Sea cucumber : (1) Five cerebrosides and one ganglioside were obtained from Cucumaria echinata and the structure was determined. (2) From Holothuria pervicax, three cerebrosides and four gangliosides were isolated and their structures were elucidated. (3) Three cerebrosides and two gangliosides were obtained and characterized from Holothuria leucoapilota. (4) The structure of three cerebrosides and one ganglioside, which were isolated from Stichopus japonicus, were determined.
3. Sea Lily : Two cerebrosides and one phosphoglycosphigolipid were obtained from the feather star Comanthus japonica and the structures were elucidated. Partial structures of two polar phosphoglycosphigolipids isolated from the feature star were also determined.
4. Biological Activities ; (1) Five cerebrosides obtained from the sea cucumber Cucumaria echinata showed lethality toward brine shrimps. (2) Seven gangliosides isolated from the starfish Luidia maculata, Linckia laevigata, and the sea cucumber Cucumaria echinata, Holothuria pervicax and Stichopus japonicus, exhibited neuritogenic activity toward the rat pheochromocytoma cell line, PC-12 cells.
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