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Development of electrodes modified with biocompatible molecules for electrochemical imaging of chemicals in biological samples

Research Project

Project/Area Number 09470494
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Physical pharmacy
Research InstitutionOsaka University

Principal Investigator

OHMORI Hidenobu  Osaka University, Graduate School of Pharmaceutical Sciences/Professor, 薬学研究科, 教授 (90028845)

Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1998: ¥1,900,000 (Direct Cost: ¥1,900,000)
Keywordsmodified electrode / biocompatibility / protein-adsorption / HPLC analysis / biological compound / electrochemical analysis / glassy carbon electrode
Research Abstract

This research has been performed, being directed to development of electrodes modified with biocompatible molecules available for electrochemical imaging of chemicals in biological samples, and has afforded the following interesting results.
1. Evaluation of electrochemical and physical performance of glassy carbon (GC) electrodes anodized in HO(CH^2CH^2O)^n R(n=l-4, R=H or CH^3) has been demonstrated that a GC electrode anodized in HO(CH^2CH^2O)^3H (Electrode 1) has the best applicability to electrochemical analysis of biological samples. By HPLC with Electrode 1, analysis of acetaminophen in urine samples is carried out with desirable sensitivity and high reproducibility without any pretreatment for removal of proteins.
2. Utilizing a GC electrode anodized successively in H20 and HO(CH^2CH^2O)^3H (Electrode 2) instead of Electrode 1, HPLC analysis of acetaminophen in urine samples can be conducted with a higher sensitivity. Electrode 2 has proved to have good electrochemical performanc … More e toward various biologically important compounds including several catecholamines, suggesting that HPLC with Electrode 2 will find a useful utility for analysis of the compounds in biological samples.
3. Coating with a polymer anodically formed from m-HOC6H4O(CH2CR2O)4CH3 has shown to serve as a tool for modification of surfaces of Au as well as GC electrodes with biocompatible molecules.
4. It was demonstrated that anodization in HO(CH^2CH^2O)^3H containing HOCH^2CH^2SO^3Na affords a GC electrode (Electrode 3) with -O(CH^2CH^2O)^2CH^2CO^2H group on its surface. Electrode 3 exhibits interesting electrochemical performance with a high selectivity toward cationic compounds including various biologically important catecholamines due to the anionic functional groups on the surface.
5. Using carboxyl groups on Electrode 3, 4-amino-2,2,6,6-tetramethylpiperidiny1-1-oxy radical (4-amino-TEMPO) or 3,4-dihydroxybeazylamine (3,4-DHBA) can be easily immobilized on the surface of a GC electrode. HPLC with the electrode having immobilized 4-amino-TEMPO is useful for analysis of sugars such as glucose at desirably low applied potential. The electrode with immobilized 3A-DHBA serves as an electrochemical mediator for NADH oxidation in the presence of Mg2^<2+>. Less

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (9 results)

All Other

All Publications (9 results)

  • [Publications] 前田初男: "Anodization in Glassy Carbon Electrodes in Oligomers of Ethylene Glycol and Their Monomethyl Ethers as a Tool for the Elimination of Protein Adsorption" Analytical Sciences. 13. 721-727 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 前田初男: "Effective Method for Covalent Introduction of 2-(2-Carboxymethoxyethoxy)ethoxy Group on a Glassy Carbon Electrode by Anodization in Triethylene Glycol" Analytical Sciences. 15(in press). (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 前田初男: "Novel Trends in Electroorganic Synthesis" Ed.by S.Torii,Springer,Tokyo, 157-160 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Hatsuo Maeda: ""Anodization in Glassy Carbon Electrodes in Oligomers of Ethylene Glycol and Their Monomethyl Ethers as a Tool for the Elimination of Protein Adsorption"" Analytical Sciences. 13. 721-727 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Hatsuo Maeda: ""Effective Method for Covalent Introduction of 2-(2-Carboxymethoxyethoxy)ethoxy Group on a Glassy Carbon Electrode by Anodization in Triethylene Glycol"" Analytical Sciences. 15(in press). (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Hatsuo Maeda: ""Modification of Glassy Carbon Surfaces by Anodic Oxidation in 1-Alkanols"" "Novel Trends in Electroorganic Synthesis"Ed.by Sigeru Torii, Springer, Tokyo. 157-160 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 山内雄二: "Electrochemical Detection of Alcohols and Carbonydrates at a Glassy Carbon Electrode Coated with a Poly(phenylene oxid)Film Containing Immabilized TEMPO" Chem.Pharm.Bull.45. 2024-2028 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] 前田初男: "Novel Trends in Electroorganic Synthesis" Springer-Verlag Tokyo(S.Torii ed.), 4/461 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 前田初男: "Anodization of Glassy Carbon Electrodes in Oligomers of Ethylene Glycol and Their Monomethyl Ethers as a Tool for the Elimination of Protein Adsorption" Analytical Sciences. 13. 721-727 (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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