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Mechanisms of the disassembly and reassembly of organelles during mitosis

Research Project

Project/Area Number 09480165
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Functional biochemistry
Research InstitutionTokyo University of Pharmacy and Life Science

Principal Investigator

TAGAYA Mitsuo  Tokyo University of Pharmacy and Life Science, School of Life Science Professor, 生命科学部, 教授 (30179569)

Co-Investigator(Kenkyū-buntansha) HATSUZAWA Kiyotaka  Tokyo University of Pharmacy and Life Science, School of Life Science assistant, 生命科学部, 助手 (20256655)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥13,200,000 (Direct Cost: ¥13,200,000)
Fiscal Year 1998: ¥4,600,000 (Direct Cost: ¥4,600,000)
Fiscal Year 1997: ¥8,600,000 (Direct Cost: ¥8,600,000)
Keywordsendoplasmic reticulum / Golgi apparatus / mitosis / membrane fusion / NSF / muclear envelope / trimeric GTP-binding protein / VCP / 核膜 / N-エチルマレイミド感受性因子
Research Abstract

At the onset of mitosis vesicle-mediated protein transport is inhibited, and organelles such as the Golgi and nuclear envelope are disassembled into small vesicles. At telophase the small vesicles are fused each other to form intact organelles. Although recent studies revealed that NSF and VCP are involved in the fusion of Golgi-derived vesicles, the mechanisms of the assembly and disassembly of organelles have not been fully understood. In the present study we studied the mechanism of the biogenesis of organelles including the nuclear envelope, Golgi apparatus, and endoplasmic reticulum. The followings are the results obtained.
1. NSF is associated with the membrane-embedded SNARE complex via SNAP.NSF is localized not only at the Golgi apparatus but also at the nuclear envelope. An antibody against a subunit of the SNARE complex, SNAP-25, inhibits the fusion of nuclear membrane vesicles in vitro, suggesting the involvement of the NSF-SNAP-SNARE complex in the nuclear membrane fusion.
2. The Golgi apparatus is disassembled by nordihydroguaiaretic acid in a mechanism dependent on trimeric GTP-binding proteins. This disassembly was suppressed by overexpression of alpha_<12> or alpha_2, but not other species, suggesting the involvement of specific alpha subunits in the organization of this organelle.
3. A novel syntaxin species (syntaxin 18) was identified. Syntaxin 18 is most likely a homologue of yeast Ufe1p, a protein involved in the fusion of nuclear or endoplasmic reticulum membranes. The role of syntaxin 18 in the organization of the endoplasmic reticulum is under investigation.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (23 results)

All Other

All Publications (23 results)

  • [Publications] Yamaguchi, T.et al.: "Possible involvement of heterotrimeric G proteins in the organization of the Golgi apparatus." J.Biol.Chem.272. 25260-25266 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nishimune, A.et al.: "NSF binding to GluR2 regulates synaptic transmission." Neuron. 21. 87-97 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Fukunaga, T.et al.: "NSF is required for the brefeldin A-promoted disassembly of the Golgi apparatus." FEBS Lett.435. 237-240 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yamaguchi, T.et al.: "ADP-ribosylation factor-1 is sensitive to N-ethylmaleimide." J.Biochem.124. 1229-1236 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tani, K.et al.: "Hypothetical protein KIAA0079 is a mammalian homologue of yeast Sec24p." FEBS Lett.in press. (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tani, K., et al.: "p125 is a novel mammalian Sec23p-interacting protein with structural similarity tophospholipid-modifying proteins." J.Biol.Chem.(in press). (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Igarashi, M.et al.: "The soluble N-ethylmaleimide-sensitive factor attached protein receptor complex in growth cones : molecular aspects of the axon terminal development." J.Neurosci.17. 1460-1470 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Takuma, T.et al.: "Evidence for the putative docking/fusion complex of exocytosis in parotid acinar cells." FEBS Lett.404. 34-36 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yamaguchi, T.et al.: "Possible involvement of heterotrimeric G proteins in the organization of the Golgi apparatus." J.Biol.Chem.272. 25260-25266 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nishimune, A.et al.: "NSF binding to GluR2 regulates synaptic transmission." Neuron. 21. 87-97 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Fukunaga, T.et al.: "NSF is required for the brefeldin A-promoted disassembly of the Golgi apparatus." FEBS Lett.435. 237-240 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yamaguchi, T.et al.: "ADP-ribosylation factor-1 is sensitive to N-ethylmaleimide." J.Biochem.124. 1229-1236 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tani, K.et al.: "Hypothetical protein KIAA0079 is a mammalian homologue of yeast Sec24p." FEBS Lett.(in press). (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tani, K., et al.: "p125 is a novel mammalian Sec23p-interacting protein with structural similarity tophospholipid-modifying proteins." J.Biol.Chem.(in press). (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Mori et al.: "Amino-terminal region of SecA is involved in the function of SecG for protein translocation into Escherichia coli membrane vesicles." J.Biochem.124. 122-129 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Nishimune et al.: "NSF binding to GluR2 regulates synaptic transmission" Neuron. 21. 87-97 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Fukunaga et al.: "NSF is required for the brefeldin A-promoted disassembly of the Golgi apparatus." FEBS Letters. 435. 237-240 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Yamaguchi et al.: "ADP-ribosylation factor-1 is sensitive to N-ethylmaleimide." J.Biochem.124. 1229-1236 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Tani et al.: "Hypothetical protein KIAA0079 is a mammalian homologue of yeast Sec24p." FEBS Letters in press. (in press).

    • Related Report
      1998 Annual Research Report
  • [Publications] Hatsuzawa, et al.: "The hydrophobic region of signal peptides is a determinant for SRP recognition and protein translocation across the ER membrane." J.Biochem.121. 270-277 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Takuma, et al.: "Evidence for the putative docking/fusion complex of exocytosis in parotid acinar cells." FEBS Lett.404. 34-36 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Igarashi, et al.: "The soluble N-ethylmaleimide-sensitive factor attachment protein receptor complexin in growth cones:molecular aspects of theaxon terminal development." J Neurosci.17. 1740-1750 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yamaguchi, et al.: "Possible involvment of heterotrimeric G proteins in the organization of the Golgi apparatuts." J.Biol.Chem.272. 25260-25266 (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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